(1) This substance stopped fetal and skin (MALME 3 line) fibroblast propagation.
(2) In maxicells, the resulting plasmid permitted tac12-promoted synthesis of two polypeptides, encoded by gene malM, with apparent molecular weights of 37 X 10(3) and 34.5 X 10(3).
(3) Smaller increases were noted with the cell lines RPMI 7951, HT 144, Malme-3M, MEL-2, and no significant increase was observed with MEL-5.
(4) Treatment of MALME-3 cells with BESPM resulted in an accumulation of N-acetylspermidine in cells and the enhanced excretion of putrescine, spermidine, and N-acetylspermidine into the medium.
(5) The malM gene, encoding a periplasmic protein of unknown function in Escherichia coli, is a highly conserved as genes encoding proteins of known function from the same region.
(6) Treatment of human MALME-3 melanoma cells with 10 microM N1,N11-bis(ethyl)norspermine (BENSPM) for 48-72 h increased SSAT activity by some 1000- to 4000-fold and enabled purification of the enzyme by established procedures--binding on immobilized spermine and elution with spermine followed by binding on Matrex Blue A and elution with coenzyme A.
(7) One malM mutation corresponding to an A.T----G.C transition showed a 10-fold-higher spontaneous reversion frequency than other such transitions in a wild-type background.
(8) From 1981, we changed a surgical procedure fro Gersony-Malm procedure to the posterior approach method, and a surgical technique from the deep hypothermia and the circulatory arrest to the moderate hypothermia and the pump perfusion.
(9) The patient was treated with repair using Gersony-Malm's method and plasty of SVC because of the stenosis of the common pulmonary vein's entrance to the SVC.
(10) In MALME-3 cells, SSAT accumulation was found to be differentially modulated by the BESPM homologues, N1,N11-bis-(ethyl)norspermine and N1,N14-bis-(ethyl)homospermine, which were 5-fold more and 9-fold less effective, respectively, than BESPM in increasing SSAT but similar in analogue uptake and effects on polyamine biosynthesis and cell growth inhibition.
(11) The intergenic region between lamB and the following gene, malM, comprises conserved segments, including one palindromic unit.
(12) They result in an increase in amount and specific activity of a MalM-LacZ hybrid protein.
(13) The complete malM open reading frame was cloned under control of the tac 12 promoter.
(14) Mutations affecting the hydrophobic core of the N-terminal extension of the MalM protein have been isolated.
(15) The calcium balance studies of Malm were re-analysed to quantify the seasonal variation he had demonstrated in one group of men.
(16) We show that the chromosomal malM gene is expressed as part of the malK-lamB operon, and that its product is periplasmic.
(17) Antiproliferative effects ranged from slowing of cell growth in the less SSAT responsive lines (LOX, SH-1) to total cessation of cell growth or overt cytotoxicity in the more potently SSAT responsive lines (MALME-3, Ebey).
(18) Finally, we demonstrate with nuclease S1 mapping experiments that the mRNA terminates at a typical rho-independent terminator located about 45 base-pairs beyond the end of gene malM, which is thus the last gene of the malK-lamB operon.
(19) We have subcloned and sequenced the genes malF and malM of Salmonella typhimurium, thereby completing the determination of the nucleotide sequence of its 'maltose B' regulon.
(20) They failed to kill autologous B cells, erythroid progenitors present in allogeneic bone marrow, and a number of cultured human tumor cells (Malme, CAKI) even after prolonged (36 h) co-culture.