What's the difference between media and supernatant?

Media


Definition:

  • (n.) pl. of Medium.
  • (n.) One of the sonant mutes /, /, / (b, d, g), in Greek, or of their equivalents in other languages, so named as intermediate between the tenues, /, /, / (p, t, k), and the aspiratae (aspirates) /, /, / (ph or f, th, ch). Also called middle mute, or medial, and sometimes soft mute.
  • (pl. ) of Medium

Example Sentences:

  • (1) Aggregation was more frequent in low-osmolal media: mainly rouleaux were formed in ioxaglate but irregular aggregates in non-ionic media.
  • (2) Rapid overgrowth of all cultures with the E. coli necessitated the use of selective media containing antimicrobial agents to which the E. coli was sensitive.
  • (3) Patient or fetal cord serum is commonly used as a protein supplement to culture media used in in-vitro fertilization (IVF).
  • (4) In addition to their involvement in thrombosis, activated platelets release growth factors, most notably a platelet-derived growth factor (PDGF) which may be the principal mediator of smooth muscle cell migration from the media into the intima and of smooth muscle cell proliferation in the intima as well as of vasoconstriction.
  • (5) The specific activities of extracts from cells grown under phototrophic and aerobic conditions were similar and not affected by the concentration of iron in the growth media.
  • (6) Concentrations of alpha-lactalbumin and alpha s1-casein were measured in media and explant homogenates by radioimmunoassay, as an index of lactogenesis.
  • (7) Prior to joining JOE Media, Will was chief commercial officer at Dazed Group, where he also sat on the board of directors.
  • (8) Statistically significant differences were found mainly in the randomized trial, where during the first and second years, respectively, adenoidectomy subjects had 47% and 37% less time with otitis media than control subjects and 28% and 35% fewer suppurative (acute) episodes than control subjects.
  • (9) Short incubations with heparin (5 min) caused a release of the enzyme into the media, while longer incubations caused a 2-8-fold increase in net lipoprotein lipase secretion which was maximal after 2-16 h depending on cell type, and persisted for 24 h. The effect of heparin was dose-dependent and specific (it was not duplicated by other glycosaminoglycans).
  • (10) Cells (1 x 10(5)) were seeded in 12- x -75-mm tissue culture tubes and incubated with various doses of IL-1 beta, IL-1 alpha, TNF-alpha, and IFN-gamma, alone or in specific combinations, for 15 min, two, 12, 24, and 72 h. PGE concentrations in the media were measured by radio-immunoassay.
  • (11) The growth of transitional epithelial cells with different growth media and growth supports was examined.
  • (12) Gamma-irradiated splenic homogenates of armadillos infected with M. leprae proved sterile by conventional tests and media.
  • (13) This can be achieved by sincere, periodic information through the mass media.
  • (14) This suggested that some of the cell population became metabolically inactive at a very early stage, possibly owing to suboptimal conditions of growth.Glycine, lysozyme and lithium chloride initiated lysis of BCG growth in the aforementioned media 24-48 hours after inoculation.
  • (15) Media made hyperosmotic with sucrose increase the frequency of spontaneously released quanta of transmitter, or miniature excitatory postsynaptic potentials (MEPSPs).
  • (16) This is not an argument for the status quo: teaching must be given greater priority within HE, but the flipside has to be an understanding on the part of students, ministers, officials, the public and the media that academics (just like politicians) cannot make everyone happy all of the time.
  • (17) The objective of this study was to examine the effects of different culture media used for maturation of bovine oocytes on in vitro embryo development following in vitro fertilization.
  • (18) This is a struggle for the survival of our nation.” As ever, after Trump’s media dressing-down, his operation was quick to fit a velvet glove to an iron fist.
  • (19) Thus Sephadex chromatography of the solution obtained by dissolving the antigen-antibody precipitate in these media repeatedly gave two peaks corresponding to anti-ovalbumin and ovalbumin.
  • (20) Cultures of Streptococcus mutans HS-6, OMZ-176, Ingbritt C, 6715-wt13, and pooled human plaque were grown in trypticase soy media with or without 1% sucrose.

Supernatant


Definition:

  • (a.) Swimming above; floating on the surface; as, oil supernatant on water.
  • (n.) The liquid remaining after solids suspended in the liquid have been sedimented by gravity or by centrifugation. Contrasted with the solid sediment, or (in centrifugation) the pellet.

Example Sentences:

  • (1) After stimulation with lipopolysaccharide and calcium ionophore A23187, culture supernatants of clones c18A and c29A showed cytotoxic activity against human melanoma A375 Met-Mix and other cell lines which were resistant to the tumor necrosis factor, lymphotoxin and interleukin 1.
  • (2) Stimulation is also observed with mixtures of APC expressing DPw3 and APC expressing A1, and likewise, DPw3+ APC become stimulatory when preincubated with supernatants from A1-positive cells.
  • (3) Gel filtration of the 40,000 rpm supernatant fraction of a homogenate of rat cerebral cortex on a Sepharose 6B column yielded two fractions: fraction II with the "Ca(2+) plus Mg(2+)-dependent" phosphodiesterase activity and fraction III containing its modulator.
  • (4) Control incubations revealed an inherent difference between the two substrates; gram-positive supernatants consistently contained 5% radioactivity, whereas even at 0 h, those from the gram-negative mutant released 22%.
  • (5) In fact, the addition of conditioned medium obtained by 48 hr preincubation of isolated monocytes with 10% PF-382 supernatant (M-CM2) or the concomitant addition of supernatant from PF-382 cells (PF-382-CM) and from unstimulated monocytes (M-CM1) are capable of fully replacing the presence of monocytes in the BFU-E assay.
  • (6) Stable factor-dependent B-cell hybridomas were used to monitor the purification of the growth factor from the supernatant of a clonotypically stimulated mouse helper T-cell clone.
  • (7) By contrast, the concentrations of IgA1 kappa and IgA1 lambda in PBMC culture supernatants, both spontaneous and PWM-stimulated, were identical in patients and controls.
  • (8) The 105 000 X g supernatant of the reaction mixture, which contained more than 85% of the thiobarbituric acid-reactive substances, did not inactivate the plasmid DNA.
  • (9) Insulin incubation of plasma membranes pretreated with protease inhibitors (leupeptin, phenylmethylsulfonylfluoride) or with exogenous trypsin, but not chymotrypsin substrates (esters of arginine and tyrosine) yields an inactive supernatant on PDH.
  • (10) Furthermore, exogenous IL-1, IL-2, IL-4, interferon-gamma, or a rat Con A supernatant failed to abrogate DFMO inhibition.
  • (11) Though no strict relationship could be observed between titers in the IH test and the time it took mice to die from the intravenous inoculation of mice (IIM test), results of the supernatants examined by both methods demonstrated that the IH test was more sensitive than the IIM one.
  • (12) The ratio of the metabolically produced Ic to Ib but not the total amount of N-oxygenated metabolites varied greatly depending of the liver microsomal fractions used in the incubation mixtures of Ia; more Ib was produced from Ia using 9000 g supernatant and conversely, more Ic was formed using the washed microsomes of the same liver.
  • (13) In healthy persons the supernatant of lymphocytes preincubated with PHA and ALG was found to show a stimulating effect to clonogenic properties of marrow progenitors, the mentioned effect being not in proportion to the concentration value.
  • (14) The particular advantage of the method described here is the ease with which the supernatants can be collected and transferred to counting vials with minimal handling of radioactive samples.
  • (15) Presence of LAF in the culture supernatants was assessed with the mouse thymocyte assay.
  • (16) Therefore, it appears that although IFN-alpha and IFN-beta are not responsible for the synergizing activity present in activated T cell supernatants, they nonetheless represent a previously unrecognized source of synergizing activity.
  • (17) When spleen cells were further cultured in vitro for 5 days in the presence of Concanavalin-A stimulated rat spleen cell supernatant (Con-A factor), CD4 or CD8 positive cells were detected in the VV (IL-6) injected group, while few positive cells were detected in the control groups.
  • (18) Strong protection was observed in animals vaccinated with 10 ml-equivalent doses of soluble, B. bovis exoantigen-containing supernatant fluids.
  • (19) Fractionation of the supernatant from ventral or dorsal hypothalamic homogenates resulted in a bimodal distribution of IR-TSH.
  • (20) The in vitro metabolism of strychnine was studied in the 9000g supernatant fractions from rat and rabbit livers.