(1) The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans.
(2) This region included the 3'-terminal part of the merA gene, merD, unidentified reading frame URF1, and a part of URF2 homologous to previously sequenced determinants of plasmid R100.
(3) No polypeptide equivalent to merD or merC of R100 was detected.
(4) The product of merD coregulates (modulates) the expression of the operon.
(5) MerD bound specifically with the mer promoter sequence.
(6) Using merD-lacZ protein fusions, we show that merD is translated.
(7) MerD protein was over-produced using a T7 expression system.
(8) merP or merD of R100 were observed on either strand.
(9) The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.
(10) All four sequenced examples of the mercury resistance (mer) operon of gram-negative bacteria have a promoter-distal reading frame, merD, whose removal has little effect on the resistance phenotype and whose translation has not previously been observed.
(11) The purified protein migrated as a 13,500 molecular weight protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the N-terminal amino acid sequence corresponded to that deduced from the DNA sequence of merD.
(12) A frame-shift mutation in merD, created by deletion of 3 bp and an insertion of a 16 bp sequence upstream of the major inverted repeats present at the 3' end of the merD sequence, resulted in increased synthesis of the structural gene transcript and higher level of resistance to Hg2+ by a factor of about 2.
(13) "Breteau is an idealist, ready put everyone in the merde ," said Jean-François Tavira, recruited by Zoe's Ark to pilot the plane, while the presiding judge described the pair's absence as an act of "grand cowardice".
(14) DNase I footprinting experiments identified a common mer operator sequence for MerR and MerD.
(15) I don't recall whether back in 1991 people quipped about Kohlrrand or Mitterohl, as everyone now does about Merkozy giving way to Merde.
(16) Merde, " exclaims the woman, but she barely breaks her stride.
(17) He's an old lady begging on a bridge, a dying man, a father picking his daughter up from a party, a motion-capture performer on a green screen, a velvety leprechaun called Monsieur Merde who flits through Père Lachaise cemetery and interrupts a fashion shoot to kidnap the model (Eva Mendes), lick her armpits and eat her hair.
(18) However, Hg(II)-induced merD expression, as measured by beta-galactosidase activity and immunoblotting, is 10- to 15-fold lower than that of fusions to the gene immediately preceding it, merA.
(19) The products of the merR and merD genes were not identified.
(20) In contrast, the synthesis rates of mRNA corresponding to the promoter-distal genes merA and merD, were initially fivefold lower than the rates of the promoter-proximal genes for the first five minutes after induction, and then rose gradually to approximately 50% of the merTPC synthesis rates.
(Superl.) Only this, and nothing else; such, and no more; simple; bare; as, a mere boy; a mere form.
Example Sentences:
(1) Interphase death thus involves a discrete, abrupt transition from the normal state and is not merely the consequence of progressive and degenerative changes.
(2) By way of major complications, merely one perforation occurred.
(3) Indeed, the nationalist and religious right bloc merely held steady , gaining just one seat.
(4) A brief review of the last decade or so of developments in health politics, policy and law suggests that health is no longer a field of mere "dynamics without change."
(5) The view that testes found lateral to the external ring and which could be pushed some way into the scrotum were merely retractile was questioned.
(6) In these three patients, laxity of the knee in flexion was so severe that posterior instability could not be corrected merely by patellar relocation.
(7) It has so far returned a mere $6m (£3.6m) of its relatively meagre $28m (£17.1m) budget, according to Forbes, a percentage of just 21%.
(8) In the literature this disease is presented merely as a metastasis.
(9) The plasmid-encoded activity does not merely replace the RecBCD enzyme failure but differs in several significant ways.
(10) Furthermore, changes between merely perceived identical parts can result in apparent depth.
(11) Thus, the long stalks of Sk1 or phosphate-starved caulobacters are not merely a function of their longer doubling times.
(12) Exogenous macromolecular DNA was able to repair, to an important degree the radiotoxic effect of 3H-thymidine on V79 cells by a mechanism other than the mere reduction of specific activity of 3H-thymidine.
(13) Multiple contacts between the gamma-subunit and calmodulin (delta-subunit), as indicated by our data, may help to explain why strongly denaturing conditions are required to dissociate these two subunits, whereas complexes of calmodulin with most other target enzymes can be readily dissociated by merely lowering Ca2+ to submicromolar concentrations.
(14) Scott insisted he was an abstract painter in the way he felt Chardin was too: the pans and fruit were uninteresting in themselves; they were merely "the means of making a picture", which was a study in space, form and colour.
(15) The charity Bite the Ballot , which persuaded hundreds of thousands to register before the last general election, is to set up “democracy cafes” in Starbucks branches, laying on experts to explain how to register and vote, and what the referendum is all about (Bite the Ballot does not take sides but merely encourages participation).
(16) These outcomes further supported the conclusion that the contextual stimuli exerted true conditional control over conditional relations in the equivalence classes and were not merely elements of compound stimuli.
(17) A mere glance at the time courses shows what reaction schemes are inapplicable.
(18) Since the discovery of the antidepressant effects of interventions in the sleep-wake cycle, a number of hypotheses have emerged according to which disturbances in sleep physiology are not merely expressions but essential components of the pathophysiology of depression.
(19) In a Facebook post , the songwriter and activist claims that Swift has merely chosen sides in the battle between Google and Spotify, saying that the singer was trying to “sell this corporate power play to us as some sort of altruistic gesture in solidarity with struggling music makers”.
(20) It is assumed that one function of grooming behaviour may be a merely cleansing one.