(1) The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans.
(2) This region included the 3'-terminal part of the merA gene, merD, unidentified reading frame URF1, and a part of URF2 homologous to previously sequenced determinants of plasmid R100.
(3) No polypeptide equivalent to merD or merC of R100 was detected.
(4) The product of merD coregulates (modulates) the expression of the operon.
(5) MerD bound specifically with the mer promoter sequence.
(6) Using merD-lacZ protein fusions, we show that merD is translated.
(7) MerD protein was over-produced using a T7 expression system.
(8) merP or merD of R100 were observed on either strand.
(9) The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.
(10) All four sequenced examples of the mercury resistance (mer) operon of gram-negative bacteria have a promoter-distal reading frame, merD, whose removal has little effect on the resistance phenotype and whose translation has not previously been observed.
(11) The purified protein migrated as a 13,500 molecular weight protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the N-terminal amino acid sequence corresponded to that deduced from the DNA sequence of merD.
(12) A frame-shift mutation in merD, created by deletion of 3 bp and an insertion of a 16 bp sequence upstream of the major inverted repeats present at the 3' end of the merD sequence, resulted in increased synthesis of the structural gene transcript and higher level of resistance to Hg2+ by a factor of about 2.
(13) "Breteau is an idealist, ready put everyone in the merde ," said Jean-François Tavira, recruited by Zoe's Ark to pilot the plane, while the presiding judge described the pair's absence as an act of "grand cowardice".
(14) DNase I footprinting experiments identified a common mer operator sequence for MerR and MerD.
(15) I don't recall whether back in 1991 people quipped about Kohlrrand or Mitterohl, as everyone now does about Merkozy giving way to Merde.
(16) Merde, " exclaims the woman, but she barely breaks her stride.
(17) He's an old lady begging on a bridge, a dying man, a father picking his daughter up from a party, a motion-capture performer on a green screen, a velvety leprechaun called Monsieur Merde who flits through Père Lachaise cemetery and interrupts a fashion shoot to kidnap the model (Eva Mendes), lick her armpits and eat her hair.
(18) However, Hg(II)-induced merD expression, as measured by beta-galactosidase activity and immunoblotting, is 10- to 15-fold lower than that of fusions to the gene immediately preceding it, merA.
(19) The products of the merR and merD genes were not identified.
(20) In contrast, the synthesis rates of mRNA corresponding to the promoter-distal genes merA and merD, were initially fivefold lower than the rates of the promoter-proximal genes for the first five minutes after induction, and then rose gradually to approximately 50% of the merTPC synthesis rates.
(2) While senator Jeff Merkely of Oregon dramatically waved his phone at Alexander during a June hearing – “What authorized investigation gave you the grounds for acquiring my cellphone data,” Merkely asked – the NSA has typically spoken in generic terms about needing the “haystack” of information from Americans it considers necessary to suss out terrorist connections.
(3) His justice minister, Beate Merk, who has refused repeated calls to resign, said she had no doubt the case had been carried out "by the book and quite correctly".
(4) Pathological alterations of the paratenon at the tendon itself are difficulty to merk off.
(5) We report such a case, which fortunately proved to be transient, and speculate on its aetiology in terms of the anatomy of the sixth cranial nerve and the possible toxic effects of the contrast agent Iopamidol (Isovue; ER Squibb and Sons, Princeton, New Jersey, USA; Niopam, E Merk, U.K.).
(6) On examination, the patient was found to have merked weakness of left limbs, spastic gait and severe impairment of touchpain- and thermosensation below the fifth cervical level but deep sensation was preserved.
(7) Forty-six received mechlorethamine (Mustargen; Merk Sharpe & Dohme, West Point, PA), vincristine (Oncovin; Eli Lilly and Company, Indianapolis), procarbazine, and prednisolone (MOPP) and 7 chlorambucil, vinblastine, prednisolone, and procarbazine (ChlVPP).
(8) 1 min: The referee, a German dentist named Marcus Merk, blows his whistle and gets proceedings underway in the 2003 Champions League final.
(9) Instead, there's likely to be a major tussle between Angela Merke l and François Hollande, with the French president expected to challenge Germany's chancellor over Berlin's hard line on austerity and foot-dragging over European banking supervision.
(10) Senator Jeff Merkely, another Democrat, said: "This really is a textbook illustration of why we need a string Volcker rule.
(11) Three sorbents were employed to establish the most appropriate conditions for the application of a fast, readily applicably and highly sensitive method--silica gel G, DG and Kiselgur "Merk", as well as 14 mobile phases--monocompound (n-hexane, n-heptane, acetone benzol and toluol), double combinations (hexane-acetone 4:1, hexane-acetone 9:1, hexane-benzol 1:1, hexane-benzol 4:1, heptane-acetone 7:1, benzol-hexane 4:1, benzol-acetone 9:1, and petrolium ether-tetrachlormethane) and triple combinations (acetone-toluol-hexane 1:15 and 5 developers (a diazosalt, bromine vapours + diazosalt, palladium bichloride, bromphenol blue, and silver nitrate, sodium hydroxide, ammonia gas).
(12) In determining these insecticides it is possible to use as a sorbent silica gel "Merk" 1:1 with the same degree of sensitivity, but with higher Rf values.
(13) The demonstration of mastitis streptococci was carried out on "TKT" agar Merk, of pathogenic staphylococci, hemolytic streptococci, and Corinebacteria--on dextrose agar Oxoid containing 7.5% citrated calf blood.