(1) The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans.
(2) This region included the 3'-terminal part of the merA gene, merD, unidentified reading frame URF1, and a part of URF2 homologous to previously sequenced determinants of plasmid R100.
(3) No polypeptide equivalent to merD or merC of R100 was detected.
(4) The product of merD coregulates (modulates) the expression of the operon.
(5) MerD bound specifically with the mer promoter sequence.
(6) Using merD-lacZ protein fusions, we show that merD is translated.
(7) MerD protein was over-produced using a T7 expression system.
(8) merP or merD of R100 were observed on either strand.
(9) The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.
(10) All four sequenced examples of the mercury resistance (mer) operon of gram-negative bacteria have a promoter-distal reading frame, merD, whose removal has little effect on the resistance phenotype and whose translation has not previously been observed.
(11) The purified protein migrated as a 13,500 molecular weight protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the N-terminal amino acid sequence corresponded to that deduced from the DNA sequence of merD.
(12) A frame-shift mutation in merD, created by deletion of 3 bp and an insertion of a 16 bp sequence upstream of the major inverted repeats present at the 3' end of the merD sequence, resulted in increased synthesis of the structural gene transcript and higher level of resistance to Hg2+ by a factor of about 2.
(13) "Breteau is an idealist, ready put everyone in the merde ," said Jean-François Tavira, recruited by Zoe's Ark to pilot the plane, while the presiding judge described the pair's absence as an act of "grand cowardice".
(14) DNase I footprinting experiments identified a common mer operator sequence for MerR and MerD.
(15) I don't recall whether back in 1991 people quipped about Kohlrrand or Mitterohl, as everyone now does about Merkozy giving way to Merde.
(16) Merde, " exclaims the woman, but she barely breaks her stride.
(17) He's an old lady begging on a bridge, a dying man, a father picking his daughter up from a party, a motion-capture performer on a green screen, a velvety leprechaun called Monsieur Merde who flits through Père Lachaise cemetery and interrupts a fashion shoot to kidnap the model (Eva Mendes), lick her armpits and eat her hair.
(18) However, Hg(II)-induced merD expression, as measured by beta-galactosidase activity and immunoblotting, is 10- to 15-fold lower than that of fusions to the gene immediately preceding it, merA.
(19) The products of the merR and merD genes were not identified.
(20) In contrast, the synthesis rates of mRNA corresponding to the promoter-distal genes merA and merD, were initially fivefold lower than the rates of the promoter-proximal genes for the first five minutes after induction, and then rose gradually to approximately 50% of the merTPC synthesis rates.
Merl
Definition:
(n.) Alt. of Merle
Example Sentences:
(1) The cases of ununited fracture of the femoral neck, treated by a technique described by Brackett (1927) and modified by Merle d'Aubigné (1968) are reported.
(2) The scores of clinical results according to the Merle d'Aubigené and Postel scale were as follows: pain 4.48, walk 3.99, movements 4.02.
(3) At an average follow-up of four years, assessment by the Merle d'Aubigńe score showed 83% excellent or good results.
(4) Rats moving freely in their cages were injected into substantia nigra with bidistilled water, with NaCl solutions of various concentrations or with a multionic Merle's solution.
(5) Fifteen patients with 17 hips have been operated and followed-up and by using the Merle d'Aubigné evaluation there was an improvement of some 3--4 points in pain, walking, and total motion.
(6) The modified Merle d'Aubigne and Postel score averaged 5.3 for pain, 3.7 for walking ability, and 4.0 for range of motion (ROM) in patients with healed infections.
(7) Between October and December 1987, a total of 232 of these patients were examined and the results rated according to Merl d'Aubigne's classification: 166 (71%) were classified as very good or good, 49 (21%) as satisfactory, and 17 (7%) as unsatisfactory.
(8) These findings are interpreted as obvious gene dosis effects of the incompletely dominant merle gene which is used to produce a characteric harlequin dappling in many breeds of dogs.
(9) In January 1960, he played the first of his celebrated prison shows at San Quentin, where one of the inmates yelling him on was Merle Haggard, locked up on a burglary charge.
(10) So-called physiological saline solution (0-9% NaCl) was found to be most toxic and the multionic Merle's solution most suitable among the control fluids tested.
(11) Based on the well known surgical techniques of Juvara, Merle d'Aubigné and J.Böhler, a precedure was presented which has been clinically tested for 6 years.
(12) Luckily for Cash, he had already met June Carter (Obituary May 17 2003), who had co-written Ring Of Fire with Merle Kilgore.
(13) Note from Dr. Merle A. Sande--We have recently come to appreciate the fact that successful chemotherapy of opportunistic infections in patients with AIDS is akin to treatment of certain malignancies: In both instances, induction, followed by maintenance, therapy is required.
(14) Ike had grown up in Muhlenberg County, Kentucky, and was a gifted guitar-picker whose playing helped to shape the playing of such country greats as Merle Travis and Chet Atkins.
(15) Radiographic and clinical assessment before and after surgery according to Ficat, Arlet and Merle D'Aubigné.
(16) Of the 77 patients in whom follow-up was possible, 76 had results falling in the very good or good categories according to the Merle d'Aubigné scheme.
(17) In 44 dachshunds of both sexes, reared and held under identical conditions in order to investigate the effects of the merle gene, ophthalmologic examinations were performed.
(18) Of the 30 hips available for functional assessment using the Merle d'Aubigné scale, 16 were excellent, 10 good, 2 fair, 1 poor and 1 bad.
(19) Most of them improved one step higher in the evaluation scheme according to Merle d'Aubigne.
(20) The syndrome may have arisen in conjunction with instability of the merle locus.