(1) The effect of the merD gene on the expression of the mer operon was determined from the rates of accumulation of merA-lacZ fusion protein in the presence and absence of an active merD gene in trans.
(2) This region included the 3'-terminal part of the merA gene, merD, unidentified reading frame URF1, and a part of URF2 homologous to previously sequenced determinants of plasmid R100.
(3) No polypeptide equivalent to merD or merC of R100 was detected.
(4) The product of merD coregulates (modulates) the expression of the operon.
(5) MerD bound specifically with the mer promoter sequence.
(6) Using merD-lacZ protein fusions, we show that merD is translated.
(7) MerD protein was over-produced using a T7 expression system.
(8) merP or merD of R100 were observed on either strand.
(9) The merD gene was cloned in a T7 promoter expression vector and the MerD protein product was visualized by autoradiography.
(10) All four sequenced examples of the mercury resistance (mer) operon of gram-negative bacteria have a promoter-distal reading frame, merD, whose removal has little effect on the resistance phenotype and whose translation has not previously been observed.
(11) The purified protein migrated as a 13,500 molecular weight protein on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and the N-terminal amino acid sequence corresponded to that deduced from the DNA sequence of merD.
(12) A frame-shift mutation in merD, created by deletion of 3 bp and an insertion of a 16 bp sequence upstream of the major inverted repeats present at the 3' end of the merD sequence, resulted in increased synthesis of the structural gene transcript and higher level of resistance to Hg2+ by a factor of about 2.
(13) "Breteau is an idealist, ready put everyone in the merde ," said Jean-François Tavira, recruited by Zoe's Ark to pilot the plane, while the presiding judge described the pair's absence as an act of "grand cowardice".
(14) DNase I footprinting experiments identified a common mer operator sequence for MerR and MerD.
(15) I don't recall whether back in 1991 people quipped about Kohlrrand or Mitterohl, as everyone now does about Merkozy giving way to Merde.
(16) Merde, " exclaims the woman, but she barely breaks her stride.
(17) He's an old lady begging on a bridge, a dying man, a father picking his daughter up from a party, a motion-capture performer on a green screen, a velvety leprechaun called Monsieur Merde who flits through Père Lachaise cemetery and interrupts a fashion shoot to kidnap the model (Eva Mendes), lick her armpits and eat her hair.
(18) However, Hg(II)-induced merD expression, as measured by beta-galactosidase activity and immunoblotting, is 10- to 15-fold lower than that of fusions to the gene immediately preceding it, merA.
(19) The products of the merR and merD genes were not identified.
(20) In contrast, the synthesis rates of mRNA corresponding to the promoter-distal genes merA and merD, were initially fivefold lower than the rates of the promoter-proximal genes for the first five minutes after induction, and then rose gradually to approximately 50% of the merTPC synthesis rates.
Nerd
Definition:
Example Sentences:
(1) The nerd may have been more in evidence early on - not least when he was doing his doctorate and ignored the advice of his Nobel prize-winning supervisor, Nikolaas Tinbergen, and opted for a stats fest, "a classic piece of Popperian science", instead of a fluffier study of animal behaviour - but it's still around.
(2) Last, and this is just a hunch as a career-long only-digital nerd: perhaps after more than a decade of digital influx, people are yearning a bit more for the physical, the tangible object, the easy-to-understand.
(3) On 5th Gear, from 2007, he talked about internet nerds reinventing themselves through social media, and offered an intentionally redneck perspective on the battle of the sexes in the deliberately gauche I'm Still a Guy.
(4) But isn't there a bit of him that wants to gloat; to tell all the kids who thought he was a nerd that he's now this babe magnet, this sex god, this… And now he really is flushed and flustered.
(5) This target audience includes not just those young people taking part in state-sponsored sports and defence training or patriotic youth groups, but also nerds who love western videogames and superheroes.
(6) "I'm a nerd who likes to keep his private life private," he said.
(7) Whereas Crow tended to rely on his rhetoric and his street-fighter smarts, Whelan is more of a data nerd – part barrow boy, part actuary.
(8) By the time kids are filling out Ucas forms or heading off to find a living, "computer stuff" has usually been relegated to the otherworldly realm of nerds.
(9) There was already a perception that Ed Miliband was too geeky, too much of an unworldly politics nerd, to have a realistic chance of power.
(10) Worldly-wise but digi-ignorant, the Vaughn-Wilson jock explosion throw in their lot with the socially maladjusted nerdly-wise virgins and soon enough, with the help of code-writing lessons for the oldies and lap dances for the virgin-geeks, they all learn to get along.
(11) And it would be nothing short of condescending for screenwriter Aaron Sorkin and director David Fincher to have concocted some fictional spunky-girl nerd character or a wise female comp sci professor in an attempt to make their film more female-friendly.
(12) "Adam had this typical nerd look: belts, tucked-in shirt.
(13) There are duelling nerds in sports jackets and a panicky associate producer (inevitably and infuriatingly female) who is smarter than her ditzy persona would have you believe.
(14) 2.16pm BST Moyes music I'm something of a music nerd, so do keep the suggestions for Dave's iPod coming.
(15) "Really in those early days the web was just for nerds like me, if you wanted some tech advice or needed to find out how to do something.
(16) And for those who think interplanetary exploration is just nonsense for space nerds, the same lessons could surely be applied closer to home.
(17) So then I grew up and I stopped taking nerd-drugs, and about the same time, Starbucks opened in the UK, whereupon I took to getting double espressos, except they were never big enough.
(18) The open source community are geeks and nerds and they created the current inequality in technology.'
(19) June 18, 2014 2.13pm BST Paul Doyle has spent the last few hours in a dark corner of the office, nerding it up on Mexico goalkeeper Guillermo Ochoa.
(20) His conclusion is a call to arms: "We need some angry nerds" – people capable of breaking out of the walled gardens.