What's the difference between microtome and microtomy?
Microtome
Definition:
(n.) An instrument for making very thin sections for microscopical examination.
Example Sentences:
(1) Enzyme activities in sections of fixed corneas were minimal in comparison with those in cold microtome sections of unfixed material revealed particularly with the technic of semipermeable membranes which is to be preferred.
(2) A reduction to about 0.60 of the microtome setting was measured in two different areas of the brain.
(3) Seventy minutes after intravenous administration, the brain is rapidly frozen and cut on a cryostate microtome.
(4) Sections, 50 microns thick, were cut on a freezing microtome.
(5) With Polycut microtome, 5 microns undecalcified sections were made and stained with 1% toluidine blue.
(6) Slices (20 microns) of cheese from the boundary layer, into which DEHA had migrated, were microtomed at -40 degrees C, and analyzed for DEHA by gas chromatography (GC).
(7) In relation to other normal histological freezer microtome, the cytological method of fine needle biopsy has the advantage of being completely without complication.
(8) Glutamate decarboxylase (GAD) activity was measured in the individual cortical layers using serial sections cut on a freezing microtome parallel to the cortical surface.
(9) The resin is polymerized at 4 C. Thin sections (1-2 microns) are obtained with a sliding microtome, and ultrathin sections (60-90 millimicrons) with a ultramicrotome.
(10) His studies into histological staining techniques (principle of elective staining, mordant staining, staining of myelin sheaths) as well as into microtome techniques proved essential to progress in pathology and bacteriology.
(11) However, there appeared to be a linear relationship between microtome setting and the thickness of dewaxed sections.
(12) Sections of the ureters, urinary bladder, and urethra were cut with a freezing microtome and the method of Kyösöla et al.
(13) A method of microtome slicing of the renal cortex was developed to standardize measurements.
(14) Of three techniques for preparing the spermatozoa, the pre-embedding method and marking of cryoultra-microtome sections proved best.
(15) Infusion of the marker followed by freezing and examination of the cut heads on a freezing microtome, shows fluorescence throughout the ventricular system, in the subarachnoid space adjacent to the posterior tela and also along the dorsal subarachnoid space of the spinal cord.
(16) This work describes the pathologic anatomy of the burst fracture both on the gross structure and also on microtome sections of the vertebrae, and examines the biomechanics of fracture reduction.
(17) They were evaluated by preparing undecalcified microtome sections and ground sections which were examined by light microscopy and microradiography.
(18) In frozen microtome sections histoenzymatic reactions were performed to detect enzymatic activity of some phosphatases and esterases.
(19) The technique, which is inexpensive, permits cutting sections or viewing surfaces from specimens up to 8 cm long, while protecting the microtome knife from damage.
(20) Sections of the frozen tissue samples are prepared on the cryostatic microtome, placed on nitrocellulose filters and hybridized with labelled DNA-probes under the conditions of RNA blot hybridization.
Microtomy
Definition:
(n.) The art of using the microtome; investigation carried on with the microtome.
Example Sentences:
(1) In three different laboratories, primary aldehyde fixation by perfusion and hot knife microtomy have given uniformly excellent data from normal, diseased, and virus-infected brain tissues.
(2) The specimen surfaces under electron microscope investigation resulted from microtomy used in the preparation of reference light microscope histological sections; thus histology served as a direct reference for the SEM and EDX analyses.
(3) adapter is easy to use, does not deform the tissue, and is the preferred method for routine microtomy.
(4) The forces acting upon the cutting blade during microtomy can be accurately measured by using a load cell.
(5) It was suggested that this might be due to the movement of extracellular material over the cells by microtomy.
(6) As shown in a series of diagrams, the localization of the microtomy artefact is a function of the orientation of the cuticle laminae relative to the knife direction, and this creates variations in the position and the extent of the microtomy effect over each lamina.
(7) One of these cryotechniques, freeze-substitution, combines the advantages of ultrarapid freezing with standard microtomy methods.
(8) They corresponded to the bow-shaped arcs seen with surface replication and conventional microtomy.
(9) This paper is a preliminary communication to illustrate the possible roles for a modified microtome in (a) the study of microtomy and (b) comparative studies of tissue density.
(10) All observations demonstrate that the clear and dark lamellae are due to a microtomy artefact which is a three-dimensional process, and not, as usually considered, due to chemical or physical variations in the structure.
(11) Three methods are evaluated for holding capsules of plastic-embedded tissue for rotary microtomy.
(12) Since the site of gamete interaction is recognizable throughout all stages of preparation, difficulties associated with locating the site of fertilization and determining specimen orientation for microtomy and electron microscopic examination are eliminated.
(13) This technique can be applied to a variety of oxide powder samples, extending the utility of microtomy as a materials science tool.
(14) Their effects on microtomy and tissue processing, and also their influence on tissue morphology and staining, were investigated.
(15) The use of SEM for capsule identification and quantification depended upon the method of sample preparation: the slide smear method was superior to samples prepared by incision or microtomy.
(16) Cutting tests of Epon-embedded material (nervous system, myelin sheat) with this knife have shown that the quality of semi-thin sections is equivalent or better than that obtained with a glass knife, and much time could be saved during the microtomy of serial sections.
(17) This approach offers several advantages over existing techniques: 1) uniform section thickness is more easily obtained than with paraffin or cryostat microtomy, thereby allowing improved resolution and more reliable identification of mesenchymal cells with small nuclei such as skeletal muscle myocytes or fibroblasts, 2) the preparations are stable over long periods and can be repeatedly viewed or photographed, and 3) calcified tissues can be examined without prior decalcification.
(18) The volume of the heart was determined multiplying the cardiac profiles areas (evaluated by point counting planimetry on serial sections) by the thickness of microtomy.
(19) This study has been made possible by two technological advances, firstly the development of cryostat microtomy for cutting unfixed, undemineralised bone, and secondly the use of scanning and integrating microdensitometry for quantifying the activity in each periosteal cell.