What's the difference between microtomy and slice?

Microtomy


Definition:

  • (n.) The art of using the microtome; investigation carried on with the microtome.

Example Sentences:

  • (1) In three different laboratories, primary aldehyde fixation by perfusion and hot knife microtomy have given uniformly excellent data from normal, diseased, and virus-infected brain tissues.
  • (2) The specimen surfaces under electron microscope investigation resulted from microtomy used in the preparation of reference light microscope histological sections; thus histology served as a direct reference for the SEM and EDX analyses.
  • (3) adapter is easy to use, does not deform the tissue, and is the preferred method for routine microtomy.
  • (4) The forces acting upon the cutting blade during microtomy can be accurately measured by using a load cell.
  • (5) It was suggested that this might be due to the movement of extracellular material over the cells by microtomy.
  • (6) As shown in a series of diagrams, the localization of the microtomy artefact is a function of the orientation of the cuticle laminae relative to the knife direction, and this creates variations in the position and the extent of the microtomy effect over each lamina.
  • (7) One of these cryotechniques, freeze-substitution, combines the advantages of ultrarapid freezing with standard microtomy methods.
  • (8) They corresponded to the bow-shaped arcs seen with surface replication and conventional microtomy.
  • (9) This paper is a preliminary communication to illustrate the possible roles for a modified microtome in (a) the study of microtomy and (b) comparative studies of tissue density.
  • (10) All observations demonstrate that the clear and dark lamellae are due to a microtomy artefact which is a three-dimensional process, and not, as usually considered, due to chemical or physical variations in the structure.
  • (11) Three methods are evaluated for holding capsules of plastic-embedded tissue for rotary microtomy.
  • (12) Since the site of gamete interaction is recognizable throughout all stages of preparation, difficulties associated with locating the site of fertilization and determining specimen orientation for microtomy and electron microscopic examination are eliminated.
  • (13) This technique can be applied to a variety of oxide powder samples, extending the utility of microtomy as a materials science tool.
  • (14) Their effects on microtomy and tissue processing, and also their influence on tissue morphology and staining, were investigated.
  • (15) The use of SEM for capsule identification and quantification depended upon the method of sample preparation: the slide smear method was superior to samples prepared by incision or microtomy.
  • (16) Cutting tests of Epon-embedded material (nervous system, myelin sheat) with this knife have shown that the quality of semi-thin sections is equivalent or better than that obtained with a glass knife, and much time could be saved during the microtomy of serial sections.
  • (17) This approach offers several advantages over existing techniques: 1) uniform section thickness is more easily obtained than with paraffin or cryostat microtomy, thereby allowing improved resolution and more reliable identification of mesenchymal cells with small nuclei such as skeletal muscle myocytes or fibroblasts, 2) the preparations are stable over long periods and can be repeatedly viewed or photographed, and 3) calcified tissues can be examined without prior decalcification.
  • (18) The volume of the heart was determined multiplying the cardiac profiles areas (evaluated by point counting planimetry on serial sections) by the thickness of microtomy.
  • (19) This study has been made possible by two technological advances, firstly the development of cryostat microtomy for cutting unfixed, undemineralised bone, and secondly the use of scanning and integrating microdensitometry for quantifying the activity in each periosteal cell.

Slice


Definition:

  • (v. t.) A thin, broad piece cut off; as, a slice of bacon; a slice of cheese; a slice of bread.
  • (v. t.) That which is thin and broad, like a slice.
  • (v. t.) A broad, thin piece of plaster.
  • (v. t.) A salver, platter, or tray.
  • (v. t.) A knife with a thin, broad blade for taking up or serving fish; also, a spatula for spreading anything, as paint or ink.
  • (v. t.) A plate of iron with a handle, forming a kind of chisel, or a spadelike implement, variously proportioned, and used for various purposes, as for stripping the planking from a vessel's side, for cutting blubber from a whale, or for stirring a fire of coals; a slice bar; a peel; a fire shovel.
  • (v. t.) One of the wedges by which the cradle and the ship are lifted clear of the building blocks to prepare for launching.
  • (v. t.) A removable sliding bottom to galley.
  • (v. t.) To cut into thin pieces, or to cut off a thin, broad piece from.
  • (v. t.) To cut into parts; to divide.
  • (v. t.) To clear by means of a slice bar, as a fire or the grate bars of a furnace.

Example Sentences:

  • (1) Microionophoretically applied excitatory amino acids induced firing of extracellularly recorded single units in a tissue slice preparation of the mouse cochlear nucleus, and the similarly applied antagonist 2-amino-5-phosphonovalerate (2APV) was demonstrated to be a selective N-methyl-D-aspartate (NMDA) receptor antagonist.
  • (2) Multiple overlapping thin 3D slab acquisition is presented as a magnitude contrast (time of flight) technique which combines advantages from multiple thin slice 2D and direct 3D volume acquisitions to obtain high-resolution cross-sectional images of vessel detail.
  • (3) This difference was abolished by exposure of the slices to propranolol, a beta-adrenergic receptor antagonist.
  • (4) All three compounds were also very similar in their effects on [3H]5HT release from superfused rat striatal slices.
  • (5) Intoxicating concentrations of ethanol also inhibit excitatory synaptic transmission mediated by N-methyl-D-aspartate receptors in hippocampal slices from adult rodents.
  • (6) The present in vitro studies show that it is found as beta-endorphin in bovine pituitary slices incubated with radioactive amino acid precursor [35S]methionine.
  • (7) This provides a direct display, in the viewing plane, of the slice profile.
  • (8) In the longitudinal direction, however, spatial resolution of under slice thickness could not be obtained.
  • (9) Tubules and cells were released from slices of kidney cortex by collagenase.
  • (10) To determine the severity of regurgitation by dynamic MRI, several parameters were analyzed, including the number of slices with visible signal loss, the time course of the signal loss, and its maximal area and maximal volume.
  • (11) This study evaluated the in vitro renin release, tissue cyclic AMP content (TcAMPc), and tissue renin content (TRC) changes with time, in response to administration of dopamine (DOP) and of the dopamine-receptor blocking agent pimozide (PIM) to renal cortical slices from sodium deficient (SD) rats.
  • (12) The Press Association tots up a total of £26bn in asset sales last year – including the state’s Eurostar stake, 30% of the Royal Mail and a slice of Lloyds.
  • (13) The effect of p-nitrophenylphosphate (p-NPP) on the release of acetylcholine evoked by drugs and ionic environments known to inhibit Na+, K+-ATPase was studied in isolated cortical slices of rat brain and longitudinal muscle strip of guinea-pig ileum.
  • (14) Prostate slices were perfused with a medium containing [(3)H]testosterone and [(14)C]androstenedione, or 5alpha-dihydro-[(3)H]testosterone and [(14)C]testosterone.
  • (15) The adenylate cyclase activator forskolin as well as 8-bromo-cyclic AMP enhanced the electrically evoked release of 3H-noradrenaline and 3H-5-hydroxytryptamine from superfused rat neocortical slices and that of 3H-dopamine from neostriatal slices with comparable EC50's of about 0.5 and 50 microM, respectively, without affecting spontaneous tritium efflux.
  • (16) Aspartate levels and release from rat striatal slices following the inhibition of glutamine synthetase (GS) by methionine sulfoximine (MSO) were studied.
  • (17) The effects of stimulus-evoked potassium release on the excitability of presynaptic axons were studied in the rat hippocampal slice preparation.
  • (18) The A1-selective agonist R-(-)N6-(2-phenylisopropyl)adenosine (10 microM) decreased 100 microM NMDA-evoked [3H]norepinephrine release by 27%; this was reversed by the P1 antagonist 8-phenyltheophylline (8-PT, 10 microM), indicating that NMDA-evoked norepinephrine release from cortical slices is susceptible to purinergic modulation.
  • (19) In vitro addition of denbufylline (10(-8)-10(-4) M) produced no significant change in [3H]choline uptake in striatal slices, while denbufylline (10(-4) M) increased high (20 mM) potassium-evoked endogenous ACh release from striatal slices.
  • (20) Under the electron microscope, slices appeared vacuolated near the cut surfaces, but well preserved internally (greater than 40 micron from the edge).

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