(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) The sensitivity of 75 non-CNS solid tumors to mismatched dsRNA was compared to the high-grade astrocytomas in the HTCA.
(3) The mismatch was incorporated into the sequence d[CGG(AP)GGC].d-(GCCACCG).
(4) One enzyme (called all-type) can nick all eight base mismatches with different efficiencies.
(5) One-year graft survival was 98% in HLA-identical grafts (n = 73), 91% in haploidentical grafts (n = 411), 89% in 2 haplotype-mismatched related grafts (n = 38), and 85% in spousal donor grafts (n = 71).
(6) Jake Schmidt, international climate policy director at the green campaigning group Natural Resources Defence Capital, said: "There's a cultural mismatch between the Qatari team and this process.
(7) Each individual hairpin forms with mismatched base pairs, one containing two GT pairs and the other containing two AC pairs.
(8) In a severe renal failure case due to obstruction, the Authors have found a great mismatch in the results between the traditional methods and the radioisotopic ones.
(9) Op72a matches the consensus sequence, whereas Op72b contains two mismatches.
(10) The relative immaturity of the lymphoid system at birth may be advantageous in decreasing the graft versus host reaction if these cells are used in a mismatched transplantation.
(11) The unusual activity of IM effector preparations against HLA-mismatched LCLs arises from fortuitous cross-recognition of allogeneic cells by immunologically specific cytotoxic T cell clones coincidentally expanded in vivo alongside the EBV-specific response.
(12) The 3 groups were comparable with respect to recipient age, duration of dialysis, prior transfusions, previous transplants, cold ischemia time, HLA AB mismatches, cytotoxic antibody profile, posttransplant ATN, and prophylactic ALG treatment.
(13) Cleavage at a total of 13 T and 21 C mismatches isolated (by at least three properly paired bases on both sides) single-base-pair mismatches was identified.
(14) It seems likely that this novel activity is involved in a broad specificity DNA repair pathway for the correction of single base mismatches in human cells.
(15) These results suggested that depressed LV function in the patients with longstanding AS was largely related to limited preload reserve due to LV enlargement and mechanical unloading of LV (correction of afterload mismatch) resulted in improvement of LV function.
(16) The above results indicate that Y-body analysis is a simple and useful tool for the demonstration of bone marrow take in sex-mismatched BMT.
(17) This finding also suggests that the Hex, Mut, and PMS systems evolved from a common ancestor and that functionally similar mismatch repair systems could be widespread among procaryotic as well as eucaryotic organisms.
(18) In contrast to rapid rejection of MHC-mismatched heart allografts, differences at non-MHC histocompatibility antigens were associated with graft survival beyond 100 days, although chronic rejection of variable severity was detected histologically.
(19) Recipients of cadaver donor transplants which were not mismatched at HLA-A,B antigens had a 10% higher graft survival rate at one year than recipients of kidneys which were completely mismatched at HLA-A,B.
(20) The transfusion effect was greater in black (8%) than white (4%) recipients; however, the 77% 1-year graft survival rate for transfused black recipients of 0 DR-mismatched kidneys did not differ from that of transfused whites.
Unsuitable
Definition:
Example Sentences:
(1) Conventional procedures for the isolation of uncontaminated polysomal RNAs which rely on sucrose density centrifugations are laborious and unsuitable for large scale isolations.
(2) However, since peptide ligands are usually unsuitable for development as potent orally active long-duration therapeutic agents, considerable research effort is being directed to the development of non-peptidal ligands.
(3) This technique can be used to treat fractures unsuited for conventional rods.
(4) Normal biomarkers are inherently unsuitable in a positive search for disorder; instead one must either use abnormal markers or be prepared to search negatively, i.e., to look for and somehow validate the rare absence of a normal marker.
(5) Seven patients judged unsuitable for an operation were treated with an aggregated allogeneic antigen.
(6) Five percent of the forceps biopsies were unsuitable for examination; all excision biopsies were of good quality.
(7) These results led to the conclusion that the IFAT screening procedure, as carried out, was unsuitable for the purposes intended.
(8) These results indicate that the 'Imotest' is significantly less sensitive than the Mantoux test and is unsuitable for use as a diagnostic or screening test.
(9) In the courts the remarks of non-specialist qualified persons can lead to wrong decisions as can either unsuitable or wrong evidence.
(10) The carboxamide group is thus unsuitable as was postulated for raising antibodies which recognize the peptide bond.
(11) Two kidneys (Group 3), deemed unsuitable for transplantation, were perfused for 24 hours with perfusate swished with unwashed sterile gloves.
(12) We conclude that s-creatinine and creatinine clearance are unsuitable measures of glomerular function during high-dose cisplatin treatment.
(13) Due to the large variations within and between days, the estimation of unconjugated oestriol in plasma might be unsuitable as a substitute for the estimation of urinary conjugated oestriol in the supervision of complicated pregnancies.
(14) The anterior superior iliac crest, the usual donor site for cortico-spongy bone grafts is unsuitable for the removal of large quantities of spongy bone.
(15) At this stage any attempt at definitive removal of diseased tissue would necessarily result in a larger dural defect at a time when local disease and systemic illness present unsuitable conditions for reparative procedures.
(16) Ultrastructural analysis indicated that Bands 2 and 3 were composed predominantly of membranes, although Band 3 was contaminated with mitochondria; Band 1 and the gradient pellet contained insufficient material and were unsuitable for ultrastructural analysis.
(17) Whilst this sensitive immunological test increases the yield of carcinomas, the high false positive rate makes it unsuitable for population screening for colorectal cancer in its present form.
(18) Unfortunately, a large number of potential compounds are unsuitable for use in dentifrices because they lack "substantivity", produce undesirable side-effects, or are incompatible with toothpaste ingredients.
(19) In this study, we have conducted a systematic investigation of various aspects of cell viability and function of isolated hepatocytes stored at 4 degrees C for 24 and 48 hr in either University of Wisconsin solution or Hanks' HEPES buffer, a control solution clinically unsuitable for organ preservation.
(20) It is concluded that heparin may be given intravenously in normal saline with benzylpenicillin, ampicillin, or methicillin but several other antibiotics were found to be unsuitable for concurrent infusion with heparin.