(1) Significant inhibition of both ComEst and MonEst was exerted by 1% sodium dodecyl sulphate (SDS) and 1.0 mM diethyl pyrocarbonate (DEPC), but the patterns of inhibition for the two esterase species with the remaining compounds studied differed considerably; for example, 0.2 mM phenylmethylsulphonyl fluoride (PMSF), 5.0 x 10(-3) M dichloroisocoumarin (DCIC) and 0.1 mM N-tosyl-L-phenylalanine chloromethyl ketone (TPCK) all inhibited MonEst but not ComEst.
(2) Analysis of inhibition kinetics further showed that (a) the reversible inhibition of both ComEst and MonEst by sodium fluoride (NaF) was noncompetitive (with Ki values of 1.28 and 0.01 mM, respectively, indicating a marked difference in sensitivity); (b) the inhibition of MonEst by PMSF was of 'mixed' noncompetitive-competitive type; and (c) that DEPC exerted noncompetitive inhibition with similar Ki values (0.05 mM) for both esterase species.
(3) These observations unequivocably demonstrate that ComEst and MonEst are unrelated enzyme species, with a common ability to hydrolyse alpha NA, and that these esterase show marked differences with respect to their active sites as adjudged by inhibitor sensitivities.
(4) Lutein, lutein monester, lutein diester, 3'-oxolutein, cryptoxanthin, zeaxanthin, beta-carotene, and zeacarotene were identified by their retention times, visible absorption spectra, behavior on saponification, and their presence or absence when lutein was the primary carotenoid fed.
(5) Mechanisms of inhibition were also examined and these studies established that SDS, PMSF, DCIC and TPCK irreversibly inactivated MonEst whilst the inhibition of ComEst by SDS was reversible.
(6) The first of these (MonEst) is specifically associated with haemopoietic cells of monocytic lineage, whereas the other species (ComEst) is common to all myeloid cells (granulocytes and monocytes) irrespective of lineage affiliation.
Monist
Definition:
(n.) A believer in monism.
Example Sentences:
(1) This essay eschews reductionist, dualist, and identity-theory attempts to resolve this problem, and offers an ontology--"monistic dual-aspect interactionism"--for the biopsychosocial model.
(2) Monistate Cream, in this study, was found to be a safe and effective drug in treating both pregnant and nonpregnant patients with confirmed candidiasis.
(3) He viewed man as a physiologist, as a materialist, and as a monist.
(4) This article briefly recapitulates the major perspectives on the problem, examines the relationship of meaning and mind to psychosocial and biological explanatory programs and to materiality, and promotes a monistic dual aspect interactionist approach to mind and body in health and illness.
(5) According to S. Freud's theories about the individual uniqueness and to G. A. Kelly's personal construct theory, the authors try to determine a monistic concept of the human being also from the clinical standpoint.
(6) Each of these possibilities offers conceptual advantages and disadvantages, so that it is difficult to adopt a monistic stance.
(7) The author discusses Freudian dualistic conception of drives and contends that it can be reduced to a monistic one, on the basis of modern conceptions of Biology, and after scanning the original writings of Freud on the subject of drive and instinct.
(8) The relationship between reading comprehension, listening comprehension, and two indicators of intelligence (verbal reasoning, speed of information processing) is analyzed on the basis of a hierarchical monistic model.
(9) Here, the old struggle between dualists and monists has awakened to new life.
(10) From Thorndike's connectionism to Pavlov's classical conditioning, Hull's monistic theory, Mowrer's two-factor theory, and Skinner's operant theory, there have been several divergent accounts of the conditions that produce imitation and the conditions under which imitation itself may facilitate language acquisition.
(11) The establishment of a bio-psycho-social (monistic) approach to all problems of human health and disease is regarded as an essential prerequisite for the improvement of medical care.
(12) This paper describes in detail two approaches to that process: monistic and pluralistic.
(13) The delay-and-antedating hypothesis does not provide a formally definitive contradiction of monist-identity theory (of the mind-brain relationship).
(14) Instead of further investigation on the problem of "unity", or on monistic or dualistic views of the mind-body problem, we propose a new theoretical approach.
(15) This report reevaluates Kohut's monistic interpretive methodology: (1) The principal features of Kohut's interpretive method are reviewed and evaluated.
(16) He differentiates between monistic theories, such as Immanuel Kant's, which rely on a single moral principle, and pluralistic theories, such as that of W.D.
(17) He believes the monistic psychobiological theory to be the most pertinent at present.