(a.) Capable of being neutralized by a univalent base or basic radical; having but one acid hydrogen atom to be replaced; -- said of acids; as, acetic, nitric, and hydrochloric acids are monobasic.
Example Sentences:
(1) It is concluded that in this cell type (i) somatostatin-14 is exclusively generated by dibasic cleavage at the Arg-2-Lys-1 site of the intact precursor with concomitant production of prosomatostatin[1-76], and (ii) no direct interactions between the monobasic and dibasic processing domains occur.
(2) At 95 degrees C in the denatured form cytosine residues titrated as a simple monobasic acid of pK3.9 compared with pK approximately 2.5 for the native form at 25 degrees C. 2.
(3) These results demonstrate that AtT-20 cells efficiently and accurately process prodynorphin at both dibasic sites and monobasic cleavage sites, indicating that the AtT-20 cells contain enzymes capable of cleaving the precursor not only at dibasic residues but also at monobasic residues.
(4) Apurinic sites were introduced to dGpdG, dGpdT, dTpdG and pdGpdG by HCl hydrolysis and the cleavage of the phosphodiester bond was measured in the monobase dinucleotides by HPLC.
(5) Additional mitomycin admixtures were reconstituted with a buffer solution containing monobasic and dibasic sodium phosphate; these were diluted with 5% dextrose injection only.
(6) Comparison of amino acids around the monobasic cleavage sites suggests that these cleavages follow certain sequence motifs and can be described as the rules that govern monobasic cleavages: (i) a basic amino acid is present at either 3, 5, or 7 amino acids N-terminal to the cleavage site, (ii) hydrophobic aliphatic amino acids (leucine, isoleucine, valine, or methionine) are never present in the position C-terminal to the monobasic amino acid at the cleavage site, (iii) a cysteine is never present in the vicinity of the cleavage site, and (iv) an aromatic amino acid is never present at the position N-terminal to the monobasic amino acid at the cleavage site.
(7) From these results it can be concluded that both the "dibasic" cleavage between vasopressin and MSEL-neurophysin and the "monobasic" cleavage between MSEL-neurophysin and copeptin occur within the granule compartment.
(8) In fact, a monobasic cleavage requires two basic residues and a domain containing nonpolar amino acids such as alanine or leucine, or both.
(9) The basic extract was neutralized and analyzed on a phenyl column eluting with acetonitrile:10 mM potassium phosphate (30:70; monobasic, pH 5.6).
(10) The method is based on derivatization with the chiral reagent 2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosyl isothiocyanate (GITC), and resolution of the resulting diastereomeric thioureas by reversed-phase HPLC using methanol-aqueous monobasic ammonium phosphate mixture as the mobile phase.
(11) dibasic and monobasic specific forms, might explain the tissue-specific processing of precursors like the pro-opiomelanocortin and the CKK and somatostatin precursor.
(12) The most abundant of these were two 39 aa forms that resulted from the monobasic endoproteolytic cleavage of proHNP-1 and proHNP-3.
(13) The monobasic macrolide erythromycin on the other hand was less affected by the SC9252 mutation, less effectively antagonized by Mg2+, and was a far less effective permeabilizer than dibasic azithromycin.
(14) In the flounder the same dibasic residue processing site is utilised but cleavage at different monobasic sites takes place (corresponding to Arg50 and Arg97 in anglerfish preprosomatostatin II).
(15) Accordingly, we evaluated the buffering capacity of seven synthetic organic buffers and monobasic potassium phosphate, both singly and in pairs, over the pH range 7.4 to 6.0.
(16) It is concluded that the processing site in the intermediate form of the pancreatic eicosapeptide is an example of a proline-directed monobasic cleavage site that has been conserved during evolution.
(17) A liquid medium, designated as Kabatiella zeae medium (KZM), containing 10.0 g of carboxymethylcellulose, 5.0 g of maltose, 1.5 g of peptone, 1.0 g of monobasic potassium phosphate, 1 L of distilled water, is described.
(18) Both peptides are synthesized as larger precursors containing paired basic and monobasic amino acids at their processing sites, which, upon cleavage, generate either SRIF-14 or -28, respectively.
(19) The monobasic processing could also be distinguished from the dibasic cleavage mechanism as, in time, the cells gradually lost the ability to cleave at the monobasic site while the dibasic processing was unaffected.
(20) It was found that the sulfonamide ligands act as monobasic bidentate ON donors.
Univalent
Definition:
(a.) Having a valence of one; capable of combining with, or of being substituted for, one atom of hydrogen; monovalent; -- said of certain atoms and radicals.
Example Sentences:
(1) Experiments indicated that complement deposition altered functionally bivalent IgG3 antibody in the immune complex into a univalent one.
(2) Equilibrium measurements of interactions of anti-DNP antibodies, prepared using DNP-PLL and several DNP-proteins for immunization, with DNP(0.6)-PLL(240) and with the univalent hapten, epsilon-DNP-L-lysine, were made utilizing the technique of fluorescence quenching.
(3) Explicit expressions are derived which describe the binding of a univalent ligand to equivalent and independent sites on each state of an acceptor undergoing indefinite self-association that is governed by an isodesmic equilibrium constant KI.
(4) A computer model was constructed which can generate all of the common univalent behaviours.
(5) In these male cells there was no decrease of chiasmata or increase of autosomal univalents with age, and there were some interstrain differences.
(6) Two homogeneous univalent hapten-protein conjugates, prepared by the covalent attachment of a single 2,4-dinitrophenyl (DNP-) or 2,4,6-trinitrophenyl (TNP-) side chain to the cysteine-SH in the active site of the enzyme papain, have been found to exhibit large Cotton effects in the wavelength region of the absorption bands of the DNP or TNP groups.
(7) Equivalent concentrations of binding sites of the bivalent F(ab)2 and univalent Fab fragments of anti-CEA were identical to the immunoglobulin G fraction in the standard inhibition curve.
(8) At a hydrophobic surface, molecules move apart and local water becomes strongly bonded, inert, and viscous and accumulates large cations, univalent anions, and compatible solutes.
(9) Of particular interest in that regard is the observation that the traditional logit-log analyses yield linear plots with the predicted slope of unity even though antigen univalence is an implicit assumption in their application.
(10) (3) The two stable states of the nerve membrane, which are readily demonstrable in TEA-treated or internally perfused squid giant axons, are shown to represent bivalent cation-rich and univalent cation-rich states of the nerve membrane.
(11) Only benzaldehyde dehydrogenase I is activated by K(+) and certain other univalent cations.
(12) Male meiosis in Mesostoma ehrenbergii ehrenbergii (2x = 10) is characterized by extreme restriction of chiasma formation; 3 pairs of chromosomes form bivalents at metaphase I which are associated by single very distally localized chiasma, while two pairs of chromosomes remain as unpaired univalents.
(13) Human red blood cells (RBCs) that are deficient in an integral membrane-associated protein ("stomatin") of apparent molecular mass 31 Kd show a catastrophic increase in passive membrane permeability to the univalent cations Na+ and K+ and are stomatocytic in shape.
(14) We demonstrate that shifts of the conductance-voltage (g-V) characteristic of PG films produced by changes of univalent or divalent cation concentrations result from changes of the membrane surface potential on one or both sides.
(15) The electrical resistances and rates of self-exchange of univalent critical ions across several types of collodion matrix membranes of high ionic selectivity were studied over a wide range of conditions.
(16) The steady-state solutions (Kirchhoff-Hill theorem) yield expressions for the relationship between the small signal conductance of univalent ions and the concentration of these ions in the external bathing medium (a saturation curve) and for the ionic currents and the steady-state current-voltage curve (N-shaped).
(17) The incidence of oocytes with univalents in the slides made by Tarkowski's method was much higher than in those made by ours in both age and strain groups (P less than 0.05-0.001).
(18) Our findings support the assumption that the altered activity of the Na-K-ATPase (modified by the presence of Ca-2+) is responsible for the univalent cation activation of the CaATPase.
(19) Under the conditions used univalent cations (Na(+),K(+) and Li(+)) inhibited the binding.
(20) The k-absorption edge spectrum of LyCuLP was consistent with the coordination of univalent copper.