What's the difference between murexide and reagent?
Murexide
Definition:
(n.) A crystalline nitrogenous substance having a splendid dichroism, being green by reflected light and garnet-red by transmitted light. It was formerly used in dyeing calico, and was obtained in a large quantities from guano. Formerly called also ammonium purpurate.
Example Sentences:
(1) Adenosine triphosphate (ATP)-dependent calcium ion uptake was measured kinetically by the absorbence changes of murexide, a metallochromic indicator of ionized Ca++ concentrations, in the absence of oxalate or other calcium-complexing anions.
(2) In 1 case the "true" initial velocity of Ca++ uptake was measured by rapid mixing of ATP in a stopped-flow apparatus and by following the change in absorbence of murexide in a storage oscilloscope.
(3) Ca2+-transport across mitochondrial membranes was measured continuously with an Aminco Dual-Wavelength-Spectrophotometer and murexide as a Ca2+-sensitive indicator.
(4) Beta-casein, at the concentrations employed, did not influence the dissociation fo calcium murexide.
(5) The apparent dissociation constant of calcium murexide is dependent upon ionic environment, ionic strength, and free calcium ion concentration.
(6) Whole casein had no effect on the absorption spectra of calcium murexide and no affinity for calcium murexide complex or murexide ion.
(7) Maximum bleaching of murexide or nitrosodimethylaniline was obtained with glycyl-glycyl-L-histidine.
(8) The Ca2+ consumption from the solution was monitored, using the color murexide reaction.
(9) In the presence of ruthenium red, both rat liver and beef heart mitochondria release sequestered Ca2+ with arsenazo III, but not with murexide.
(10) These indicators are analogues of the purpurate components of murexide and tetramethylmurexide, in which two acetate groups have been incorporated into each molecule to render it membrane impermeant.
(11) Bioptic confirmation of diagnosis is therefore usually very difficult if a murexide reaction test cannot be carried out concurrently.
(12) The decrease in absorbance at 510 nm of the murexide-calcium complex, due to the addition of DMDP, was used as a reliable (s.d.
(13) Several thiol compounds (GSH, cysteine, and cysteamine), pyridine nucleotides (NADH, NADPH) and ascorbic acid were also effective in generating the murexide-derived free radical.
(14) 17:13 1974), the absorbance changes of murexide caused by Ca2+ and followed up by a dual wavelength spectrophotometer were applied to measure synaptosomal Ca2+-binding in the presence of cations such as Rb+, Mn2+ or La3+.
(15) The determination with murexide of free and protein-bound calcium in model systems of known composition, ionic strength, and pH was investigated.
(16) Rates of Ca2+ uptake were monitored by two methods: measurement of initial rates of proton ejection following CaCl2 additions and measurement of disappearance of Ca2+ from the suspension medium using murexide as indicator in a dual wavelength spectrophotometer.
(17) When exposed to oxygen radicals, murexide is converted to a colorless alloxan derivative and its absorbance at 520 nm decreases in proportion to the radicals produced.
(18) This method is based on the competition for calcium binding by either DMDP or murexide.
(19) Moreover, murexide bleaching is inhibited in the presence of radical scavengers, allowing a comparison of their scavenging activities.
(20) Using the murexide (5,5'-nitrilodibarbituric acid ammonium salt) bleaching technique, we attempted to spectroscopically determine whether there are any histidylpeptides-Ni (II) complexes able to catalyze a nickel-dependent reduction of hydrogen peroxide leading to free oxygen radical production.
Reagent
Definition:
(n.) A substance capable of producing with another a reaction, especially when employed to detect the presence of other bodies; a test.
Example Sentences:
(1) At 36 h postsurgery, RBCs were examined by 23Na-NMR by using dysprosium tripolyphosphate as a chemical shift reagent.
(2) It includes preincubation of diluted plasma with ellagic acid and phospholipids and a starting reagent that contains calcium and a chromogenic peptide substrate for thrombin, Tos-Gly-Pro-Arg-pNA.
(3) The assumption was also corroborated using reagents from a family in which DR3 and DQw2 were not found in the usually described linkage.
(4) The ADAM derivative of carnitine was separated from decomposition products of the reagent and related compounds such as amino acid derivatives on a silica gel column eluted with methanol-5% aqueous SDS-phosphoric acid (990:10:1).
(5) The activity is strongly inhibited by SH-blocking reagents (e.g.
(6) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
(7) Loss of reduced protein thiols, as measured by binding of the thiol reagent iodoacetic acid to GPD, and loss of GPD enzymatic activity occurred in a dose-dependent manner.
(8) Cost-effective immunoassays for the detection of amphetamines, benzodiazepines, and methadone in urine have been developed using Syva EMIT reagents and a Cobas Bio centrifugal analyser.
(9) The presence of a previously unreported dipeptide transport mechanism within blood leukocytes and the selective enrichment of the granule enzyme, DPPI, within cytotoxic effector cells of lymphoid or myeloid lineage appear to afford a unique mechanism for the targeting of immunotherapeutic reagents composed of simple dipeptide esters or amides.
(10) In the highest concentration tested, two other reagents 2,4-dimethoxybenzylamine and N-acetylimidazole, did not influence the activity of the steroisomers on aorta.
(11) On the other hand, the hydrophilic reagents, 1-ethyl-3-[3-(dimethylamino)-propyl]carbodiimide and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline, did not affect organic cation transport.
(12) The histidine-specific reagent diethylpyrocarbonate (DEP) was used to chemically modify TNF.
(13) Subgingival plaque was sampled and the presence or absence of the above mentioned bacteria assessed with BANA reagent cards (Perio Scan).
(14) Histochemical and immunocytochemical staining of the outgrowths with reagents that depict epithelial, myoepithelial, and lactating alveolar cells (peanut lectin alone, monoclonal and polyclonal antibodies to rat caseins) indicate similar cell compositions and arrangements for all outgrowths irrespective of their source; these are also similar to the mammary glands of the perphenazine-stimulated or lactating hosts.
(15) Adhesion and O2- production were also found to be differentially affected by the NADPH oxidase inhibitor diphenylene iodonium, the sulfhydryl reagent N-ethylmaleimide and the A2 agonist adenosine, indicating that these neutrophil responses have various transductional pathways that also depend on the type of stimulus.
(16) Either reagent dislocates FAD from the holoenzyme, leaving a characteristic mercaptide derivative of the apoenzyme.
(17) Since all five reagents are sensitive to the LA, then the best overall reagent will be the one with the best sensitivity to the most important need for the laboratory (usually heparin monitoring).
(18) The enzyme was inhibited by thiol and carbonyl reagents.
(19) Radiation inactivation and simple target theory were employed to determine the molecular weight of an insect CNS alpha-bungarotoxin binding component in the presence and absence of a cross-linking reagent, dimethyl suberimate.
(20) The probes were biotin treated by chemical modification of the DNA by the peraminating reagent or photochemically.