(1) However, an anti-nef antibody response was also seen in 5 of 93 (5%) nonrisk dermatological patients and in 4 of 37 (11%) healthy blood donors.
(2) Plasma was analyzed for NEF and 3 metabolites [hydroxynefazodone (HO-NEF), m-chlorophenylpiperazine (mCPP) and p-hydroxynefazodone (p-HO-NEF)] by a validated HPLC assay.
(3) We found that HIV Nef shares sequence and structural features with scorpion peptides known to interact with K+ channels.
(4) These observations were confirmed by in vitro experiments using purified NeFs.
(5) DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef.
(6) Complement-independent binding of C3 nephritic factor (NEF) to sheep erythrocytes was observed in heat-inactivated sera from patients having this autoantibody.
(7) In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1.
(8) Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge.
(9) The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography.
(10) Earlier studies indicated nef to be a negative regulatory factor for viral transcription, whereas more recent studies report evidence against this original hypothesis.
(11) Absence of anti-nef antibodies was associated with symptomatic HIV infection.
(12) The effects of Nef on the transmembrane potential were studied in rabbit sinoatrial nodes and the clusters of cultured cardiac myocytes from neonatal rats.
(13) The type 1 human immunodeficiency virus (HIV-1) encodes a 27-kDa protein termed Nef (negative factor).
(14) Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
(15) Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides.
(16) In contrast, the extent and the appearance of nef-specific antibodies during the course of infection varied considerably between individual animals.
(17) The functions of yet other viral genes (nef, vpt) remain unclear.
(18) All of these anti-nef positive sera also contained antibodies directed against viral structural proteins.
(19) The results showed that the vascular selectivity values of Ver, Nef, and Tet were 1.15, 0.32, and 0.20, respectively in WKY and 0.80, 0.24, and 0.10, respectively in SHR.
(20) Recombinant antigens containing all or parts of the HIV-1 proteins p24, Nef and gp41 and HIV-2 gp36 have been purified and used to develop a rapid immunoassay to detect and differentiate between HIV-1 and HIV-2 antibodies in a single test.
Ner
Definition:
(adv. & a.) nearer.
Example Sentences:
(1) The Nurses Evaluation Rating Scale (NERS) consists of 16 items designed to capture salient dimensions of psychopathology and nursing care requirements for psychiatric patients.
(2) Using electrophoretic mobility shift assays (EMSA) it is shown that nuclear extracts from young radish leaves (NER) contain a protein fraction which binds to specific sequences in the radish ES.
(3) At the time of E2-capsule removal (0 h) large increases in nuclear estradiol receptor (NER) levels were seen in anterior pituitary, preoptic area, and hypothalamus (HYP).
(4) Correspondingly, although the hypothalamic NER levels were equally elevated 24 hr after either a low or a high dose of EB, these receptor concentrations remained high at 36 and 48 hr post EB, only in those animals that received the high estrogen dose.
(5) A good friend and NER supporter was quick to remind me that SKC's 3-0 win at Sporting Park in August against his team was a drama-filled event where two NER players saw red in the second half.
(6) Breast cancer tissue from 95 women was simultaneously assayed for three receptors: cytosolic estrogen (CER), cytosolic progesterone (CPR), and nuclear estrogen (NER).
(7) By adapting a method for DNA-footprinting using impure extracts of ner overproducing cells, we were able to determine that the ner-binding sites are located between nucleotides 1026 and 1058 from the Mu left end.
(8) The Ner protein of bacteriophage Mu negatively regulates transcription initiated at the early promoter and at the major repressor promoter.
(9) High resistance to mastitis was determined in the progeny of AO-4, EM-01, NC-17, NB-10, NEB-15, NUN-3, Nx-33 bulls, while opposite results were recorded in the progeny of NAR-45, NAR-47, NER-01, NOM-19 and REN-100 bulls.
(10) Using a number of criteria, a generalized ranking order of the toxicity of the compounds was drawn up: TRA > ETR > ACIT > 13CRA > 4HPR > TZR identical to NBR > NER identical to 13CNER.
(11) Black patients whose tumors contained both CER and NER had a significantly better time to recurrence than those whose tumors lacked these receptors, while in Asian women the presence of CER alone, or CER together with NER, or CER, NER, and CPR, was indicative of a significantly longer disease-free period.
(12) TMF contains leucine-zipper amino acid motifs and exhibits homology in its DNA binding domain with the phage-encoded DNA binding protein Ner.
(13) The content of UEBP (NUEBP) and ER (NER) in the liver were determined using the quantitative methods for differential specific determination of the E2-binding sites of these proteins.
(14) Irrigation with the Ner river water increased the copper content of the soil ten times.
(15) The NERS appears to be a promising instrument for longitudinal, daily evaluations of inpatient psychopathology as seen in the routine clinical practice of psychiatric nurses.
(16) Among 25 patients with CER- the rare occurrence of NER+ in only three patients did not suggest any clinical implication.
(17) Both teams have a lot of pride at stake, and as I review side-by-side stats from the regular season rounded to whole percentages, the two lead in shutouts, and are close to even on passing accuracy (SKC's 78% to NER's 76%) and duels won (SKC's 50% to NER's 48%).
(18) NER worked hard to win a playoff spot, (the first since 2009), and SKC has evolved over the season even if Vermes is criticized for rarely changing tactics.
(19) The main objective was to determine whether the addition of NER assay to the currently accepted practice with only CER and CPR could improve the predictive capacity of receptors.
(20) Results indicated that NER levels changed significantly throughout pregnancy and that the magnitude and direction of change was site specific.