(1) However, an anti-nef antibody response was also seen in 5 of 93 (5%) nonrisk dermatological patients and in 4 of 37 (11%) healthy blood donors.
(2) Plasma was analyzed for NEF and 3 metabolites [hydroxynefazodone (HO-NEF), m-chlorophenylpiperazine (mCPP) and p-hydroxynefazodone (p-HO-NEF)] by a validated HPLC assay.
(3) We found that HIV Nef shares sequence and structural features with scorpion peptides known to interact with K+ channels.
(4) These observations were confirmed by in vitro experiments using purified NeFs.
(5) DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef.
(6) Complement-independent binding of C3 nephritic factor (NEF) to sheep erythrocytes was observed in heat-inactivated sera from patients having this autoantibody.
(7) In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1.
(8) Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge.
(9) The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography.
(10) Earlier studies indicated nef to be a negative regulatory factor for viral transcription, whereas more recent studies report evidence against this original hypothesis.
(11) Absence of anti-nef antibodies was associated with symptomatic HIV infection.
(12) The effects of Nef on the transmembrane potential were studied in rabbit sinoatrial nodes and the clusters of cultured cardiac myocytes from neonatal rats.
(13) The type 1 human immunodeficiency virus (HIV-1) encodes a 27-kDa protein termed Nef (negative factor).
(14) Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
(15) Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides.
(16) In contrast, the extent and the appearance of nef-specific antibodies during the course of infection varied considerably between individual animals.
(17) The functions of yet other viral genes (nef, vpt) remain unclear.
(18) All of these anti-nef positive sera also contained antibodies directed against viral structural proteins.
(19) The results showed that the vascular selectivity values of Ver, Nef, and Tet were 1.15, 0.32, and 0.20, respectively in WKY and 0.80, 0.24, and 0.10, respectively in SHR.
(20) Recombinant antigens containing all or parts of the HIV-1 proteins p24, Nef and gp41 and HIV-2 gp36 have been purified and used to develop a rapid immunoassay to detect and differentiate between HIV-1 and HIV-2 antibodies in a single test.
Net
Definition:
(v. t.) To make into a net; to make n the style of network; as, to net silk.
(v. t.) To take in a net; to capture by stratagem or wile.
(v. t.) To inclose or cover with a net; as, to net a tree.
(v. i.) To form network or netting; to knit.
(a.) Without spot; pure; shining.
(a.) Free from extraneous substances; pure; unadulterated; neat; as, net wine, etc.
(a.) Not including superfluous, incidental, or foreign matter, as boxes, coverings, wraps, etc.; free from charges, deductions, etc; as, net profit; net income; net weight, etc.
(v. t.) To produce or gain as clear profit; as, he netted a thousand dollars by the operation.
Example Sentences:
(1) Manometric studies with resting cells obtained by growth on each of these sulfur sources yielded net oxygen uptake for all substrates except sulfite and dithionate.
(2) M NET is currently installed in referring physician office sites across the state, with additional physician sites identified and program enhancements under development.
(3) External exposures to a contaminated fishing net and fishing boat are considered pathways for fishermen.
(4) If tracer is introduced into the carotid artery after osmotic treatment, brain uptake is increased by a net factor of 50 (a factor of 70 due to elevation of PA, multiplied by 7 due to infusion by the carotid route) as compared to uptake by normal, untreated brain with infusion into a peripheral vein.
(5) Short incubations with heparin (5 min) caused a release of the enzyme into the media, while longer incubations caused a 2-8-fold increase in net lipoprotein lipase secretion which was maximal after 2-16 h depending on cell type, and persisted for 24 h. The effect of heparin was dose-dependent and specific (it was not duplicated by other glycosaminoglycans).
(6) Only those derivatives with a free amino group and net positive charge in the side chain were effective.
(7) When labelled long-chain fatty acids or glycerol were infused into the lactating goat, there was extensive transfer of radioactivity into milk in spite of the absence of net uptake of substrate by the mammary gland.
(8) PYY inhibited the reduction in net absorption of sodium chloride and water evoked by vasoactive intestinal peptide (VIP), but did not affect the VIP-evoked increase in net potassium secretion.
(9) A relative net reduction of 47% in lactose malabsorption was produced by adding food, and the peak-rise in breath H2 was delayed by 2 hours.
(10) In assessing damaged nets and curtains it must be recognised that anything less than the best vector control may have no appreciable impact on holoendemic malaria.
(11) No net hepatic uptake of glucose was observed before or after feeding.
(12) This force will be numerically similar to the net driving Starling force in small pores, but distinctly different in large pores.
(13) Increased amino acid incorporation into hepatic proteins in tumor-bearing animals and also probably in cancer patients is due to a net increased hepatic protein synthesis, probably not confined to acute-phase reactants only.
(14) In this study, protein efficiency ratio and net protein utilization together with the kinetic estimates of protein turnover were used to compare the effect of different protein and fat sources in healthy rats.
(15) Meanwhile the Brooklyn Nets, who have been dealing with nothing but bad news since the start of the regular season, will be without Paul Pierce for 2-4 weeks, also due to a right hand fracture.
(16) In the postprandial state net acid (4.9%) and sulfate (2.2%) had much less importance as determinants of calciuria.
(17) Proper maintenance of body orientation was defined to be achieved if the net angular displacement of the head-and-trunk segment was zero during the flight phase of the long jump.
(18) The authors tested their own technique, using transplants or implants of corium, fascia, dura mater and polyester net, internally in the tendons, fastening them with an external cross suture.
(19) These studies indicate that, in three models of acute liver injury, the net influx of calcium across the plasma membrane is increased early in the evolution of the injury before irreversible damage occurs.
(20) A state of net secretory fluid flux was induced in isolated jejunal loops in weanling pigs by adding theophylline or cholera toxin to the lumen of the isolated loops.