(1) However, an anti-nef antibody response was also seen in 5 of 93 (5%) nonrisk dermatological patients and in 4 of 37 (11%) healthy blood donors.
(2) Plasma was analyzed for NEF and 3 metabolites [hydroxynefazodone (HO-NEF), m-chlorophenylpiperazine (mCPP) and p-hydroxynefazodone (p-HO-NEF)] by a validated HPLC assay.
(3) We found that HIV Nef shares sequence and structural features with scorpion peptides known to interact with K+ channels.
(4) These observations were confirmed by in vitro experiments using purified NeFs.
(5) DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef.
(6) Complement-independent binding of C3 nephritic factor (NEF) to sheep erythrocytes was observed in heat-inactivated sera from patients having this autoantibody.
(7) In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1.
(8) Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge.
(9) The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography.
(10) Earlier studies indicated nef to be a negative regulatory factor for viral transcription, whereas more recent studies report evidence against this original hypothesis.
(11) Absence of anti-nef antibodies was associated with symptomatic HIV infection.
(12) The effects of Nef on the transmembrane potential were studied in rabbit sinoatrial nodes and the clusters of cultured cardiac myocytes from neonatal rats.
(13) The type 1 human immunodeficiency virus (HIV-1) encodes a 27-kDa protein termed Nef (negative factor).
(14) Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
(15) Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides.
(16) In contrast, the extent and the appearance of nef-specific antibodies during the course of infection varied considerably between individual animals.
(17) The functions of yet other viral genes (nef, vpt) remain unclear.
(18) All of these anti-nef positive sera also contained antibodies directed against viral structural proteins.
(19) The results showed that the vascular selectivity values of Ver, Nef, and Tet were 1.15, 0.32, and 0.20, respectively in WKY and 0.80, 0.24, and 0.10, respectively in SHR.
(20) Recombinant antigens containing all or parts of the HIV-1 proteins p24, Nef and gp41 and HIV-2 gp36 have been purified and used to develop a rapid immunoassay to detect and differentiate between HIV-1 and HIV-2 antibodies in a single test.
New
Definition:
(superl.) Having existed, or having been made, but a short time; having originated or occured lately; having recently come into existence, or into one's possession; not early or long in being; of late origin; recent; fresh; modern; -- opposed to old, as, a new coat; a new house; a new book; a new fashion.
(superl.) Not before seen or known, although existing before; lately manifested; recently discovered; as, a new metal; a new planet; new scenes.
(superl.) Newly beginning or recurring; starting anew; now commencing; different from has been; as, a new year; a new course or direction.
(superl.) As if lately begun or made; having the state or quality of original freshness; also, changed for the better; renovated; unworn; untried; unspent; as, rest and travel made him a new man.
(superl.) Not of ancient extraction, or of a family of ancient descent; not previously kniwn or famous.
(superl.) Not habituated; not familiar; unaccustomed.
(superl.) Fresh from anything; newly come.
(adv.) Newly; recently.
(v. t. & i.) To make new; to renew.
Example Sentences:
(1) The liver metastasis was produced by intrasplenic injection of the fluid containing of KATOIII in nude mouse and new cell line was established using the cells of metastatic site.
(2) The femoral component, made of Tivanium with titanium mesh attached to it by a new process called diffusion bonding, retains superalloy fatigue strength characteristics.
(3) Apparently, the irradiation with visible light of a low intensity creates an additional proton gradient and thus stimulates a new replication and division cycle in the population of cells whose membranes do not have delta pH necessary for the initiation of these processes.
(4) If ascorbic acid was omitted from the culture medium, the extensive new connective tissue matrix was not produced.
(5) Neuropsychological testing is a relatively new field in the area of clinical neuroscience.
(6) says Gregg Wallace opening the new series of Celebrity MasterChef (Mon-Fri, 2.15pm, BBC1).
(7) A new balloon catheter has been developed for angioplasty.
(8) A new and simple method of serotyping campylobacters has been developed which utilises co-agglutination to detect the presence of heat-stable antigens.
(9) These results suggest the presence of a new antigen-antibody system for another human type C retrovirus related antigens(s) and a participation of retrovirus in autoimmune diseases.
(10) The combined analysis of pathogenesis and genetics associated with the salmonella virulence plasmids may identify new systems of bacterial virulence and the genetic basis for this virulence.
(11) The previous year, he claimed £1,415 for two new sofas, made two separate claims of £230 and £108 for new bed linen, charged £86 for a new kettle and kitchen utensils and made two separate claims, of £65 and £186, for replacement glasses and crockery.
(12) Richard Bull Woodbridge, Suffolk • Why does Britain need Chinese money to build a new atomic generator ( Letters , 20 October)?
(13) This new observation offers good possibilities to study the metabolism of tryptophan at the cellular level.
(14) Graft life is even more prolonged with patch angioplasty at venous outflow stenoses or by adding a new segment of PTFE to bypass areas of venous stenosis.
(15) Paradoxically, each tax holiday increases the need for the next, because companies start holding ever greater amounts of their tax offshore in the expectation that the next Republican government will announce a new one.
(16) Michael Schumacher’s manager hopes F1 champion ‘will be here again one day’ Read more Last year, Red Bull were frustrated by Mercedes, Ferrari and Honda as they desperately looked for a new engine supplier.
(17) The strongest predictor of non-sudden cardiac death was the New York Heart Association functional class.
(18) But RWE admitted it had often only been able to retain customers with expired contracts by offering them new deals with more favourable conditions.
(19) In the fall of 1975, 1,915 children in grades K through eight began a school-based program of supervised weekly rinsing with 0.2 percent aqueous solution of sodium fluoride in an unfluoridated community in the Finger Lakes area of upstate New York.
(20) Schneiderlin, valued at an improbable £27m, and the currently injured Jay Rodriguez are wanted by their former manager Mauricio Pochettino at Spurs, but the chairman Ralph Krueger has apparently called a halt to any more outgoings, saying: “They are part of the core that we have decided to keep at Southampton.” He added: “Jay Rodriguez and Morgan Schneiderlin are not for sale and they will be a part of our club as we enter the new season.” The new manager Ronald Koeman has begun rebuilding by bringing in Dusan Tadic and Graziano Pellè from the Dutch league and Krueger said: “We will have players coming in, we will make transfers to strengthen the squad.