(1) However, an anti-nef antibody response was also seen in 5 of 93 (5%) nonrisk dermatological patients and in 4 of 37 (11%) healthy blood donors.
(2) Plasma was analyzed for NEF and 3 metabolites [hydroxynefazodone (HO-NEF), m-chlorophenylpiperazine (mCPP) and p-hydroxynefazodone (p-HO-NEF)] by a validated HPLC assay.
(3) We found that HIV Nef shares sequence and structural features with scorpion peptides known to interact with K+ channels.
(4) These observations were confirmed by in vitro experiments using purified NeFs.
(5) DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef.
(6) Complement-independent binding of C3 nephritic factor (NEF) to sheep erythrocytes was observed in heat-inactivated sera from patients having this autoantibody.
(7) In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1.
(8) Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge.
(9) The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography.
(10) Earlier studies indicated nef to be a negative regulatory factor for viral transcription, whereas more recent studies report evidence against this original hypothesis.
(11) Absence of anti-nef antibodies was associated with symptomatic HIV infection.
(12) The effects of Nef on the transmembrane potential were studied in rabbit sinoatrial nodes and the clusters of cultured cardiac myocytes from neonatal rats.
(13) The type 1 human immunodeficiency virus (HIV-1) encodes a 27-kDa protein termed Nef (negative factor).
(14) Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
(15) Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides.
(16) In contrast, the extent and the appearance of nef-specific antibodies during the course of infection varied considerably between individual animals.
(17) The functions of yet other viral genes (nef, vpt) remain unclear.
(18) All of these anti-nef positive sera also contained antibodies directed against viral structural proteins.
(19) The results showed that the vascular selectivity values of Ver, Nef, and Tet were 1.15, 0.32, and 0.20, respectively in WKY and 0.80, 0.24, and 0.10, respectively in SHR.
(20) Recombinant antigens containing all or parts of the HIV-1 proteins p24, Nef and gp41 and HIV-2 gp36 have been purified and used to develop a rapid immunoassay to detect and differentiate between HIV-1 and HIV-2 antibodies in a single test.