(1) However, an anti-nef antibody response was also seen in 5 of 93 (5%) nonrisk dermatological patients and in 4 of 37 (11%) healthy blood donors.
(2) Plasma was analyzed for NEF and 3 metabolites [hydroxynefazodone (HO-NEF), m-chlorophenylpiperazine (mCPP) and p-hydroxynefazodone (p-HO-NEF)] by a validated HPLC assay.
(3) We found that HIV Nef shares sequence and structural features with scorpion peptides known to interact with K+ channels.
(4) These observations were confirmed by in vitro experiments using purified NeFs.
(5) DEAE-transfection of viral DNA containing an open from of nef yielded virus that replicated with similar kinetics and to a similar extent in macaque peripheral blood lymphocyte (PBL) cultures as virus with a deletion or stop codon within nef.
(6) Complement-independent binding of C3 nephritic factor (NEF) to sheep erythrocytes was observed in heat-inactivated sera from patients having this autoantibody.
(7) In 31 of the sera, the specificity of anti-nef antibodies was further analysed by enzyme-linked immunosorbent assay (ELISA) with large synthetic peptides ranging from 31 to 66 amino acid residues and spanning the total sequence of nef from HIV-1.
(8) Human T-lymphocytic cell line H9 infected with the HTLV-IIIB isolate of human immunodeficiency virus type 1 (HIV-1) synthesizes two forms of the Nef protein (p25 and p27) that differ both in molecular weight and charge.
(9) The recombinant Nef protein was purified to near homogeneity by DEAE-Sephacel, phenyl-Sepharose 4B, blue-Sepharose, and Sephadex G-150 column chromatography.
(10) Earlier studies indicated nef to be a negative regulatory factor for viral transcription, whereas more recent studies report evidence against this original hypothesis.
(11) Absence of anti-nef antibodies was associated with symptomatic HIV infection.
(12) The effects of Nef on the transmembrane potential were studied in rabbit sinoatrial nodes and the clusters of cultured cardiac myocytes from neonatal rats.
(13) The type 1 human immunodeficiency virus (HIV-1) encodes a 27-kDa protein termed Nef (negative factor).
(14) Surprisingly, CTL specific for regulatory, non-structural nef protein appear to be a major constituent of the human immune response to HIV.
(15) Determination of the B-cell epitopes of the nef molecule encoded by the human immunodeficiency virus type 1 (HIV-1) was undertaken using a set of six synthetic peptides.
(16) In contrast, the extent and the appearance of nef-specific antibodies during the course of infection varied considerably between individual animals.
(17) The functions of yet other viral genes (nef, vpt) remain unclear.
(18) All of these anti-nef positive sera also contained antibodies directed against viral structural proteins.
(19) The results showed that the vascular selectivity values of Ver, Nef, and Tet were 1.15, 0.32, and 0.20, respectively in WKY and 0.80, 0.24, and 0.10, respectively in SHR.
(20) Recombinant antigens containing all or parts of the HIV-1 proteins p24, Nef and gp41 and HIV-2 gp36 have been purified and used to develop a rapid immunoassay to detect and differentiate between HIV-1 and HIV-2 antibodies in a single test.
Ship
Definition:
(n.) Pay; reward.
(n.) Any large seagoing vessel.
(n.) Specifically, a vessel furnished with a bowsprit and three masts (a mainmast, a foremast, and a mizzenmast), each of which is composed of a lower mast, a topmast, and a topgallant mast, and square-rigged on all masts. See Illustation in Appendix.
(n.) A dish or utensil (originally fashioned like the hull of a ship) used to hold incense.
(v. t.) To put on board of a ship, or vessel of any kind, for transportation; to send by water.
(v. t.) By extension, in commercial usage, to commit to any conveyance for transportation to a distance; as, to ship freight by railroad.
(v. t.) Hence, to send away; to get rid of.
(v. t.) To engage or secure for service on board of a ship; as, to ship seamen.
(v. t.) To receive on board ship; as, to ship a sea.
(v. t.) To put in its place; as, to ship the tiller or rudder.
(v. i.) To engage to serve on board of a vessel; as, to ship on a man-of-war.
(v. i.) To embark on a ship.
Example Sentences:
(1) Some commentators have described his ship, now facing more delays after a decade in development, as little more than a Heath Robinson machine.
(2) Total costs of building the three missile destroyers in Australia will amount to more than $9bn, approximately three times the cost of buying the ships ready made from Spanish company Navantia, The Australian reported on Friday .
(3) The Italian coastguard ship Bruno Gregoracci docked in Malta at about 8am and dropped off two dozen bodies recovered from this weekend’s wreck, including children, according to Save the Children.
(4) There were members of the smuggling gang on the ship with walkie-talkies.
(5) Already Britain's electricity is becoming too dependent on gas brought in by ship through the Suez canal.
(6) The goal of the expedition, led by Prof Ken Takai of the Japan Agency for Marine-Earth Science and Technology, was to study the limits of life at deep-sea vents in the Cayman Trough as part of a round-the-world voyage of discovery by the research ship RV Yokosuka .
(7) The risk for gastric cancer and non-malignant respiratory disease among the workers of the coke shipping department was increased but the SMRs did not reach statistical significance.
(8) The plan to round up some business and ship away seemed sound.
(9) The US has stopped shipping military equipment out of Afghanistan , citing the risk to truckers from protests along part of the route in neighbouring Pakistan.
(10) Polish foreign affairs minister Radoslaw Sikorski has opposed the ships being handed over.
(11) The 61-year-old Canadian, who was one of the original founders of Greenpeace , was arrested last Sunday at Frankfurt airport at the request of Costa Rica, which wants to see him extradited over a 10-year-old charge of "violating ships traffic".
(12) I don’t do the social media myself, so who knows.” The Pentagon said the drone, also described as a “glider” or unmanned underwater vehicle, was deployed by civilian contractors aboard the USNS Bowditch, a scientific research ship.
(13) The main animal paramyxoviruses are parainfluenza 3 (agent of shipping fever) in cattle; NDV (cause of fowl pest) and Yucaipavirus in birds; Sendai and PVM in mice; Nariva virus in rodents; possibly bovinerespiratory syncytial virus; and SV5 and SV41 in monkeys.
(14) Vigils have been held in Cairo for the victims of EgyptAir flight 804 as a French navy ship headed to join the deep-sea search in the Mediterranean for the main wreckage and flight recorders.
(15) The source of the first outbreak was monkeys shipped from Africa; the origin of the second episode is unclear.
(16) Ships should be able to sail directly over the north pole by the middle of this century, considerably reducing the costs of trade between Europe and China but posing new economic, strategic and environmental challenges for governments, according to scientists.
(17) Rob DiGiovanni, who heads a marine mammal rescue group on Long Island, said he was seeing "more evidence of ship strikes and that's definitely a concern".
(18) An improved membrane filtration procedure for use on board ship to enumerate Escherichia coli and Group D faecal streptococci in marine sediments is described.
(19) Official estimates suggest the number of small packages shipped into Europe more than quadrupled from 26m in 2000 to 115m two years ago.
(20) The survey ship has been used in the Gulf of Aden monitoring the Somali coastline, as well as scientific missions such as mapping the seabed of the Persian Gulf.