(1) Analysis of a mutation at +60 confirms that the UAS is required for efficient NIFA-mediated activation of nifF transcription.
(2) The promoter sequences of nifLA, nifB and nifF in K. oxytoca coincided exactly with those of K. pneumoniae in the consensus regions at -12 and -26, although the overall homology in the promoter regions was 96%.
(3) The nifF product thus belongs to the long-chain class of flavodoxins.
(4) Complementation analysis of millimicron-induced, amber, frameshift, and deletion mutations indicates there are five polycistronic and two monocistronic operons: nifQ nifB, nifA nifL, nifF, nifM nifV nifS, nifN nifE, nifK nifD nifH, and nifJ.
(5) The nifF gene coding for the flavodoxin from the nitrogen-fixing bacterium Azotobacter vinelandii (strain OP) was cloned into the plasmid vector pUC7 [Bennett, L. T., Jacobsen, M. R., & Dean, D. R. (1988) J. Biol.
(6) Most mutants could be assigned to one of seven nif cistrons: nifA, nifB, nifD, nifE, nifF, nifH, and nifK.
(7) The results of nifF-lacZYA gene fusion experiments and Northern hybridization analyses indicated that the nifF gene is both transcribed and translated under nitrogen fixing and non-nitrogen fixing conditions.
(8) DR from a nifF- background was also susceptible to ADP-ribosylation, indicating that the oxidized form of DR will serve as a substrate for DRAT in vivo.
(9) These genes include nifH, nifD, nifK, nifT, nifY, nifE, nifN, nifX, nifU, nifS, nifV, nifW, nifZ, nifM, and nifF.
(10) The nifF gene of Klebsiella pneumoniae was cloned into a multicopy plasmid in order to construct a strain that synthesizes and retains an elevated concentration of the gene product relative to the wild-type strain.
(11) Southern hybridization analysis revealed that there is only a single copy of the nifF gene on the A. vinelandii OP genome.
(12) However, under nitrogen fixing conditions a substantial increase in both nifF synthesis and in accumulation of an approximately 800-base pair nifF-encoding mRNA species was observed.
(13) The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH.
(14) The regulatory region spanning the divergently transcribed nifF and nifLA promoters contains a NIFA-specific upstream activator sequence (UAS) located around +59, and two NTRC binding sites centred at -142 and -163 with respect to the nifLA transcription start site.
(15) A nucleotide sequence showing extensive homology to the nifF gene, which codes for a flavodoxin involved in nitrogen fixation in Klebsiella pneumoniae, was localized on the plasmid pEA3 of Enterobacter agglomerans and determined.
(16) The flavodoxin was purified using FPLC and resolved into two forms, designated KpFldI and KpFldII, which were shown to have identical N-terminal amino acid sequences (30 residues) in agreement with that predicted by the K. pneumoniae nifF DNA sequence.
(17) NtrC bound to both of these ntr repressible promoters with equal affinity, but did not bind to the activatable nitrogen fixation promoters nifF or nifLA.
(18) 15% of its soluble cytoplasmic protein as a flavodoxin, the Klebsiella pneumoniae nifF gene product, has been constructed.
(19) Mutant strains were constructed which have an insertion mutation or an insertion and a deletion mutation within the nifF gene coding sequence.
(20) These data demonstrate that the nifF-encoded flavodoxin of A. vinelandii OP, although not essential for nitrogen fixation, is required for maximum in vivo nitrogenase activity.
Pong
Definition:
Example Sentences:
(1) The kinetic data are compatible with a tert-uni ping-pong mechanism, as in the case of the 'classical' glutathione peroxidase (EC 1.11.1.9).
(2) Initial-velocity kinetic studies indicate the enzyme acts by a ping-pong mechanism.
(3) Initial velocity and isotope exchange studies confirmed that the over-all reaction, like that catalyzed by pyruvate carboxylase purified from rat liver and chicken liver, was a nonclassical Ping Pong Bi Bi Uni Uni sequence with ATP and HCO3-binding randomly in the Bi Bi partial reaction.
(4) The asymptotic kinetics of lipoamide oxidoreductions switch between the ping pong and ordered mechanisms by varying pH of the reactions.
(5) Penetration of these drugs into perforated ping pong balls implanted intraperitoneally in rabbits was studied.
(6) The results obtained were consistent with a ping-pong or substitution mechanism.
(7) Since each catalytic cycle step is irreversible, the data fit a peroxidase ping-pong mechanism rather than an ordered bi-bi ping-pong mechanism.
(8) And then, proving that in the celebrity world of self-abasement there really is no such thing as "bottoming out", Shane started tweeting Ping Pong, otherwise known as Elizabeth Hurley's parrot Why has Australia not staged an intervention?
(9) It seems most probable that, as previously suggested by others for Ts, eIF-2B effectively catalyses an exchange reaction through a "ping-pong" type mechanism.
(10) Initial velocity studies indicate that the enzymatic reaction proceeds by a Ping-Pong mechanism.
(11) This reaction pathway is compared with the double displacement (Ping Pong) mechanisms that have previously been described for pyruvate carboxylases from other sources.
(12) The wild-type enzyme and Y177F mutant displayed ping-pong kinetics, but the Y177S and Y177G mutants appeared to have switched to an ordered sequential mechanism.
(13) But I don’t think [Lords chief whip] Ben Stoneham is going to be very accommodating to anyone.” Brexit weekly briefing: article 50 moves closer but EU dashes divorce deal hopes Read more Labour has promised no “extended ping pong” as it does not want to frustrate the timetable for triggering article 50, but it has laid eight amendments on issues from EU nationals to quarterly reporting to parliament about the Brexit process.
(14) Initial velocity measurements in which the concentration of L-alpha-methyl-gamma-glutamyl-L-alpha-aminobutyrate was varied at several fixed acceptor concentrations, and either the release of alpha-aminobutyrate or the formation of the transpeptidation products was determined, yielded results which are consistent with a ping-pong mechanism modified by a hydrolytic shunt.
(15) Contrary to findings with another enzyme (fraction I), a Bi Uni Uni Bi Ping Pong mechanism (Cleland, 1963a,b,c) corresponding to Berg's (1956) scheme of reaction was eliminated and an ordered Ter Ter mechanism with an A-C-B (standing for ATP, CoA and butyrate respectively) sequence of substrate entry for the overall reaction was established for fraction II.
(16) The Peak Ia and Peak II enzymes both absolutely require alpha-D-glucose 1,6-bisphosphate and Mg2+ for activity, and appear to have a "ping-pong" mechanism.
(17) Neuronal as well as astrocytic GABA-transaminase obeyed a bi bi ping-pong reaction mechanism.
(18) The N-acetylglucosamine and mannitol enzymes II, which do not function with an enzyme III, catalyze sugar phosphorylation by a ping-pong mechanism when the two substrates are phospho-HPr and sugar.
(19) Constant-ratio double-reciprocal plots confirm that the enzyme mechanism is nonsequential (ping-pong bi-bi).
(20) In contrast, a comparable reaction of the A-U-G analog with 30S subunits lead to a predominant crosslinking of A-U-G to proteins S4 and S12 (Pongs, O., Stoffler, G.A., Lanka, E., (1975) J. Mol.