(1) Analysis of a mutation at +60 confirms that the UAS is required for efficient NIFA-mediated activation of nifF transcription.
(2) The promoter sequences of nifLA, nifB and nifF in K. oxytoca coincided exactly with those of K. pneumoniae in the consensus regions at -12 and -26, although the overall homology in the promoter regions was 96%.
(3) The nifF product thus belongs to the long-chain class of flavodoxins.
(4) Complementation analysis of millimicron-induced, amber, frameshift, and deletion mutations indicates there are five polycistronic and two monocistronic operons: nifQ nifB, nifA nifL, nifF, nifM nifV nifS, nifN nifE, nifK nifD nifH, and nifJ.
(5) The nifF gene coding for the flavodoxin from the nitrogen-fixing bacterium Azotobacter vinelandii (strain OP) was cloned into the plasmid vector pUC7 [Bennett, L. T., Jacobsen, M. R., & Dean, D. R. (1988) J. Biol.
(6) Most mutants could be assigned to one of seven nif cistrons: nifA, nifB, nifD, nifE, nifF, nifH, and nifK.
(7) The results of nifF-lacZYA gene fusion experiments and Northern hybridization analyses indicated that the nifF gene is both transcribed and translated under nitrogen fixing and non-nitrogen fixing conditions.
(8) DR from a nifF- background was also susceptible to ADP-ribosylation, indicating that the oxidized form of DR will serve as a substrate for DRAT in vivo.
(9) These genes include nifH, nifD, nifK, nifT, nifY, nifE, nifN, nifX, nifU, nifS, nifV, nifW, nifZ, nifM, and nifF.
(10) The nifF gene of Klebsiella pneumoniae was cloned into a multicopy plasmid in order to construct a strain that synthesizes and retains an elevated concentration of the gene product relative to the wild-type strain.
(11) Southern hybridization analysis revealed that there is only a single copy of the nifF gene on the A. vinelandii OP genome.
(12) However, under nitrogen fixing conditions a substantial increase in both nifF synthesis and in accumulation of an approximately 800-base pair nifF-encoding mRNA species was observed.
(13) The order obrained was hisD nifB nifA (nifL) nifF nifE nifK nifD nifH.
(14) The regulatory region spanning the divergently transcribed nifF and nifLA promoters contains a NIFA-specific upstream activator sequence (UAS) located around +59, and two NTRC binding sites centred at -142 and -163 with respect to the nifLA transcription start site.
(15) A nucleotide sequence showing extensive homology to the nifF gene, which codes for a flavodoxin involved in nitrogen fixation in Klebsiella pneumoniae, was localized on the plasmid pEA3 of Enterobacter agglomerans and determined.
(16) The flavodoxin was purified using FPLC and resolved into two forms, designated KpFldI and KpFldII, which were shown to have identical N-terminal amino acid sequences (30 residues) in agreement with that predicted by the K. pneumoniae nifF DNA sequence.
(17) NtrC bound to both of these ntr repressible promoters with equal affinity, but did not bind to the activatable nitrogen fixation promoters nifF or nifLA.
(18) 15% of its soluble cytoplasmic protein as a flavodoxin, the Klebsiella pneumoniae nifF gene product, has been constructed.
(19) Mutant strains were constructed which have an insertion mutation or an insertion and a deletion mutation within the nifF gene coding sequence.
(20) These data demonstrate that the nifF-encoded flavodoxin of A. vinelandii OP, although not essential for nitrogen fixation, is required for maximum in vivo nitrogenase activity.
Smell
Definition:
(n.) To perceive by the olfactory nerves, or organs of smell; to have a sensation of, excited through the nasal organs when affected by the appropriate materials or qualities; to obtain the scent of; as, to smell a rose; to smell perfumes.
(n.) To detect or perceive, as if by the sense of smell; to scent out; -- often with out.
(n.) To give heed to.
(v. i.) To affect the olfactory nerves; to have an odor or scent; -- often followed by of; as, to smell of smoke, or of musk.
(v. i.) To have a particular tincture or smack of any quality; to savor; as, a report smells of calumny.
(v. i.) To exercise the sense of smell.
(v. i.) To exercise sagacity.
(v. t.) The sense or faculty by which certain qualities of bodies are perceived through the instrumentally of the olfactory nerves. See Sense.
(v. t.) The quality of any thing or substance, or emanation therefrom, which affects the olfactory organs; odor; scent; fragrance; perfume; as, the smell of mint.
Example Sentences:
(1) • young clownfish will lose their ability to "smell" the anemone species that they shelter in.
(2) Earlier recognition of foul-smelling mucoid discharge on the IUD tail, or abnormal bleeding, or both, as a sign of early pelvic infection, followed by removal of the IUD and institution of appropriate antibiotic therapy, might prevent the more serious sequelae of pelvic inflammation.
(3) The lid is fiddly to fit on to the cup, and smells so strongly of silicone it almost entirely ruins the taste of the coffee if you don’t remove it.
(4) A history and physical examination focused on signs and symptoms of chemosensory disorders, in combination with screening tests for taste and smell function, can quickly and easily delineate the general type and cause of the dysfunction.
(5) The coke sailed up my nasal passage, leaving behind the delicious smell of a hot leather car seat on the way back from the beach.
(6) "When I burp and pass wind the smell is absolutely horrendous.
(7) Examination of illustrative case reports demonstrates that the qualitative features of the Odorant Confusion Matrix offer additional insights to support etiologic diagnoses of disturbances in sense of smell.
(8) Receptor cells with cilia were observed, and although the olfactory system undergoes further differentiation during pouch life and although the olfactory epithelium and bulb of the newborn differs from that of the adult, these facts do not preclude the ability of the newborn to detect smell.
(9) Donna Sinclair, head of Options 4 Change, a charity working with black youths "I went to Brixton the morning after and the smell of burning, and what you could see told a huge story.
(10) 'The smell had become unbelievably bad by then', she said.
(11) Anything that good for you might be expected to smell foul and come in a medicine bottle, but the Mediterranean diet is generally considered to be delicious, except by those who hate olive oil.
(12) The media, smelling blood, has fallen into pack formation.
(13) It might smell close to pot, he said, but would be “tainted” because of all the other items and plants like poison oak burning along with it.
(14) Data from each subject were fitted by a compartmental model for zinc metabolism that was developed previously for patients with taste and smell dysfunction.
(15) Learning of the motor to the mother smell seems to occur yet in prenatal period.
(16) Guardian US environment correspondent Suzanne Goldenberg looked at the role cities would have to play in reducing emissions: At-risk cities hold solutions to climate change: UN report It is already taking shape as the 21st century urban nightmare: a big storm hits a city like Shanghai, Mumbai, Miami or New York, knocking out power supply and waste treatment plants, washing out entire neighbourhoods and marooning the survivors in a toxic and foul-smelling swamp.
(17) A sensory world beyond the care setting can be evoked through recorded birdsong or the smell of flowers.
(18) Similar messages delivered by previous populist, independent candidates like Ralph Nader and Ross Perot didn’t catch on because there was always that whiff of ego that voters like me could smell, coupled with lack of experience in government.
(19) Of the group returning the tests, seven employees reported having smell problems due to allergies or sinus disease.
(20) This is payback, without a doubt.” The workers recently won the support of Will Self, who supported a boycott of the venue, writing : “If the punters wake up and smell the crap coffee of corporate greed, perhaps we won’t be so keen on contributing to those revenues.