(1) These studies led to the following conclusions: (a) all the prominent NHP which remain bound to DNA are also present in somewhat similar proportions in the saline-EDTA, Tris, and 0.35 M NaCl washes of nuclei; (b) a protein comigrating with actin is prominent in the first saline-EDTA wash of nuclei, but present as only a minor band in the subsequent washes and on washed chromatin; (c) the presence of nuclear matrix proteins in all the nuclear washes and cytosol indicates that these proteins are distributed throughout the cell; (d) a histone-binding protein (J2) analogous to the HMG1 protein of K. V. Shooter, G.H.
(2) The fine structure of neurofibrillary tangles in the hippocampal gyrus, substantia nigra, pontine nuclei and locus coeruleus of the brain was postmortem studied in a case of progressive supranuclear palsy.
(3) Two small populations of GLY + neurons were observed outside of the named nuclei of the SOC; one was located dorsal to the LSO, near its dorsal hilus, and the other was identified near the medial pole of the LSO.
(4) A topographic relationship was recognized between the MM and the anterior thalamic nuclei.
(5) Diplotene spermatocytes have the largest nuclei of all germ cells.
(6) The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei.
(7) DNA in situ is progressively denatured when the cells or nuclei are treated with increasing concentration of acridine orange (AO).
(8) The deep cerebellar nuclei were moderately labeled at birth and gradually decreased in density thereafter.
(9) Mitoses of nuclei of myocytes of the left ventricle of the heart observed in two elderly people who had died of extensive relapsing infarction are described.
(10) The ability of cytoplasmic extracts to induce DNA synthesis in isolated, quiescent nuclei.
(11) In high concentrations of antiserum, some of the agglutinated cells of L. h. hertigi were enlarged and showed syncytial characters that included up to five nuclei, two dividing nuclei and five basal bodies associated with a single kinetoplast.
(12) The responses of adrenocorticotropin (ACTH), renin, epinephrine and norepinephrine and arterial pressure and heart rate (HR) to hypotensive hemorrhage were examined before and 1 h after lesion of the paraventricular nuclei (PVN) in pentobarbital-anesthetized rats and 1 day before and 4 days after lesion of the PVN in conscious rats.
(13) The anatomic and functional development of the suprachiasmatic nuclei (SCN) was studied in the gray short-tailed opossum, Monodelphis domestica.
(14) The rate of nuclei stained by Pr-122 is different from that of Pr-192 in both growing and quiescent cultures.
(15) Using a monoclonal antibody against dopamine and a rabbit antiserum against serotonin, 5-methoxytryptamine or tryptamine, we were able to achieve the simultaneous localization of two amines in glutaraldehyde-fixed sections of rat dorsal raphe nuclei.
(16) The results obtained further knowledge of the anatomy of the nuclei, specifically the areas used for the prosthesis implantation and the underlying tissue.
(17) The phosphorylation pattern was affected by the addition of cholera toxin or GDP beta S to the isolated nuclei.
(18) While the heaviest anterogradely labeled ascending projections were observed to the contralateral ventral posterolateral nucleus of the thalamus, pars oralis (VPLo), efferent projections were also observed to the contralateral ventrolateral thalamic nucleus (VLc) and central lateral (CL) nucleus of the thalamic intralaminar complex, magnocellular (and to a lesser extent parvicellular) red nucleus, nucleus of Darkschewitsch, zona incerta, nucleus of the posterior commissure, lateral intermediate layer and deep layer of the superior colliculus, dorsolateral periaqueductal gray, contralateral nucleus reticularis tegmenti pontis and basilar pontine nuclei (especially dorsal and peduncular), and dorsal (DAO) and medial (MAO) accessory olivary nuclei, ipsilateral lateral (external) cuneate nucleus (LCN) and lateral reticular nucleus (LRN), and to a lesser extent the caudal medial vestibular nucleus (MVN) and caudal nucleus prepositus hypoglossi (NPH), and dorsal medullary raphe.
(19) No HRP-labeled axons were found in the facial and solitary nuclei and the cerebellum.
(20) The reaction of cis-diamminedichloroplatinum(II) with chicken erythrocyte nuclei produces covalent cross-linking of HMG proteins 1, 2 and E to DNA, in addition to cross-links amongst LMG proteins.
Pronuclei
Definition:
(pl. ) of Pronucleus
Example Sentences:
(1) Fertilization was recognized by the presence of a second polar body and two pronuclei.
(2) Different intracytoplasmic positioning of the nuclei treated with ND (pronuclei, synkarya and two products of the first division) shows that most of cell cytoplasm is competent for inducing macronuclear development.
(3) Microtubule assembly is required for the formation of the male and female pronuclei during mouse, but not sea urchin, fertilization.
(4) One of the remaining eight had two pronuclei 18 hr after microinjection and progressed to a four-cell embryo after 48 hr.
(5) Sperm nuclei injected into maturing oocytes formed condensed chromatin fragments as long as the oocytes were not activated, but they transformed into pronuclei when the oocytes were stimulated with electric shock.
(6) To obtain information on the remodeling of sperm chromatin during male pronuclei formation, we have followed the sperm specific histones (SpH) that form the nucleosomal core by Western immunoblot analysis with polyclonal antibodies directed against the core SpH.
(7) There were 90 oocytes with more than two pronuclei (4.7% of fertilized oocytes), identified in 72 cycles (11.4% of cycles).
(8) The lost SpH's are not replaced by newly synthesized CS variants, since the basic proteins synthesized de novo during male pronuclei formation are not incorporated into chromatin remaining in the cytoplasm.
(9) Prolonged in vitro culture of oocytes after parthenogenetic activation and diploidization treatment showed that formed pronuclei were able to fuse and single prometaphase to telophase mitotic cleavage figures developed in all oocytes fixed 28 h after activation, except one cytochalasin B-treated oocyte with two prometaphase sets of chromosomes.
(10) Oocytes possessing at least two pronuclei were transferred to recipient CD-1 females which had been mated 24 hr earlier to vasectomized males.
(11) Introduction of a kinase-inhibitory anti-mos antibody into mouse zygotes 12 h after fertilization prevented the first cleavage of zygotes at the pronuclei stage.
(12) This is followed by a close association or fusion of male and female pronuclei (2, 13, 15, 16).
(13) DNA synthesis was shown to begin in pronuclei when they are still in the marginal zones of cytoplasm, immediately after their formation.
(14) The potential for nuclear fusion is not restricted to pronuclei alone since diploid nuclei in binucleate cells could be fused using centrifugation in solutions of Colcemid to bring the nuclei into apposition.
(15) If the latter occurs between pronuclei from different secondary oocytes, heterozygosity can be maintained.
(16) The obscured pronuclei or nuclei in living one- and two-celled pig ova were revealed after centrifugation for 3 min at 15,000 X g. To determine viability of centrifuged ova, one- and two-celled pig ova were collected from superovulated gilts; half of the ova were centrifuged and all ova were transferred into recipient gilts.
(17) OA-induced nuclear envelope breakdown in pronuclei as well as in second PB nuclei.
(18) Karyoplast containing pronuclei was transferred into the perivitelline space of the enucleated zygote and fused to cytoplasm with electrofusion.
(19) However, development to the blastocyst stage of enucleated eggs receiving pronuclei from untreated eggs was as good as that of control reconstituted eggs when the cytoplasm originated from eggs exposed to u.v.
(20) However, pronuclei derived from hamster sperm nuclei were more likely to be synthesizing DNA at 6 hr than pronuclei derived from sperm nuclei of other species.