(1) Using a silver staining technique (AgNOR technique), we have investigated the nucleolar organizer-associated proteins (NORs) in formalin-fixed paraffin embedded conjunctival specimens of 15 intraepithelial squamous carcinomas, 10 hyperplastic-dysplastic samples and 10 control epithelial fragments; the mean number of intranuclear black dots was determined for each case.
(2) We have cloned and sequenced a DNA fragment from the genome of Drosophila melanogaster which is homologous to Novikoff hepatoma (rat) small nucleolar U3 RNA.
(3) They continuously produced heteropolymeric G6PD and showed strictly additive patterns of silver staining of both parental sets of nucleolar organizing chromosomes.
(4) After treatment, an increase in the nucleolar volume is observed.
(5) Both the usual nucleolar and non-nucleolar transcription complexes were visualized.
(6) When protein biosynthesis is inhibited by either cycloheximide of puromycine, the nucleolar RNA synthesis of Ehrlich ascites tumor cells decreases by approximately 70% within 1 h, while the removal of these protein synthesis inhibitors causes a rapid recovery of nucleolar RNA synthesis, largely within 1 h. A similar pattern of decrease and recovery of endogenous RNA polymerase activity in isolated nucleoli or in nuclei (in the presence of alpha-amanitin) may be demonstrated after addition and removal of these drugs.
(7) The two characteristic forms of eucaryotic DNA-dependent RNA polymerases, polymerase I (nucleolar) and polymerase II (nucleoplasmic), were identified.
(8) The morphologic basis for a nucleolar-cytoplasmic interrelationship via the nucleolar channel system is described.
(9) If purified nuclei were heated for 45 min at 37 degrees C, the final matrix exhibited well-recognizable nucleolar remnants, an inner network and a peripheral lamina.
(10) It is present on all seven rye chromosomes and hybridizes to the entire length of each chromosome, with the exception of some telomeres and the nucleolar organiser region.
(11) The description of the structure of epithelial cells includes: 1) the endoplasmic reticulum and ribosomes, 2) mitochondria, 3) the nucleus, 4) the golgi complex and secretory bodies, 5) lysosomes, 6) annulate lamellae, 7) luminal surface, 8) basal surface, 9) lateral surface, and 10) the nucleolar channel system.
(12) Changes in the nucleolar structure were noticed in hepatocytes administered both suramin and CCl4.
(13) We studied 20 gastric adenocarcinoma to determine DNA ploidy pattern by flow cytometry and nucleolar organizer regions by the colloidal silver method.
(14) Some of these genotypes are aneuploids with deletions or additions of chromosomes bearing nucleolar organisers.
(15) Nucleolar modifications induced by herpes simplex virus type 1 (HSV1) infection were studied at the ultrastructural level with special attention to the fate of a family of proteins serologically related to the nucleolar 100 kDa protein.
(16) The phosphatase was capable of dephosporylating the major phosphorylated nucleolar proteins C23-24 and B23-24 and also histone H1.
(17) Nucleolar organizer regions (NORs) are loops of DNA encoded for ribosomal RNA production.
(18) We analyzed the number and aspect of nucleolar organizer regions in 11 specimens from ampullary carcinoma, by means of silver colloidal staining.
(19) Nucleolar organizer regions are collections of nucleolar proteins associated with ribosomal genes that can be visualized in histologic sections using a silver colloid stain, thus the term silver-staining nucleolar organizer region (AgNOR).
(20) The results show that DHT induces a significant nucleolar enlargement but intranuclear migration of rRNA is not apparently affected by androgens; migration of RNA through euchromatin is delayed by castration and stimulated by DHT; migration through the nuclear envelope is androgen-dependent.
Nucleus
Definition:
(n.) A kernel; hence, a central mass or point about which matter is gathered, or to which accretion is made; the central or material portion; -- used both literally and figuratively.
(n.) The body or the head of a comet.
(n.) An incipient ovule of soft cellular tissue.
(n.) A whole seed, as contained within the seed coats.
(n.) A body, usually spheroidal, in a cell or a protozoan, distinguished from the surrounding protoplasm by a difference in refrangibility and in behavior towards chemical reagents. It is more or less protoplasmic, and consists of a clear fluid (achromatin) through which extends a network of fibers (chromatin) in which may be suspended a second rounded body, the nucleolus (see Nucleoplasm). See Cell division, under Division.
(n.) The tip, or earliest part, of a univalve or bivalve shell.
(n.) The central part around which additional growths are added, as of an operculum.
(n.) A visceral mass, containing the stomach and other organs, in Tunicata and some mollusks.
Example Sentences:
(1) Microionophoretically applied excitatory amino acids induced firing of extracellularly recorded single units in a tissue slice preparation of the mouse cochlear nucleus, and the similarly applied antagonist 2-amino-5-phosphonovalerate (2APV) was demonstrated to be a selective N-methyl-D-aspartate (NMDA) receptor antagonist.
(2) The present results provide no evidence for a clear morphological substrate for electrotonic transmission in the somatic efferent portion of the primate oculomotor nucleus.
(3) In the caudal spinal trigeminal nucleus (Vc), the collaterals of one half of the periodontium afferent fibers terminated mainly in lamina V at the rostral and middle levels of Vc.
(4) It is concluded that in the mouse model the ability of buspirone to reduce the aversive response to a brightly illuminated area may reflect an anxiolytic action, that the dorsal raphe nucleus may be an important locus of action, and that the effects of buspirone may reflect an interaction at 5-hydroxytryptamine receptors.
(5) hypoglossal nucleus), whereas in other areas the angiotensinogen gene was expressed only in a certain population of glial cells.
(6) Local application of 8-OH-DPAT (0-5 micrograms) into the median raphe nucleus, facilitated male rat sexual behavior, as evidenced by a decrease in number of intromissions preceding ejaculation and in time to ejaculation.
(7) In addition, lightly immunostained cells were distinguished in the caudal portion of the hypothalamic arcuate nucleus, area of tuber cinereum, retrochiasmatic area, and rostral portion of the paraventricular thalamic nucleus after colchicine treatment.
(8) Arising of the nucleus from a polyheteronomous nucleoid of proeukaryotes.
(9) It is possible that the IgE that linked abnormally with the propofol had specific binding sites for the phenyl nucleus and the isopropyl groups, which are present in propofol and many other drugs.
(10) We conclude that neuronal activities in the region of the retrofacial nucleus are important both in the integration of stimuli from the central chemoreceptors and in defining the discharge patterns of respiratory neurons.
(11) A 68 year-old man with a history of right thalamic hemorrhage demonstrated radiologically in the pulvinar and posterior portion of the dorsomedian nucleus developed a clinical picture of severe physical sequelae associated with major affective, behavioral and psychic disorders.
(12) While the heaviest anterogradely labeled ascending projections were observed to the contralateral ventral posterolateral nucleus of the thalamus, pars oralis (VPLo), efferent projections were also observed to the contralateral ventrolateral thalamic nucleus (VLc) and central lateral (CL) nucleus of the thalamic intralaminar complex, magnocellular (and to a lesser extent parvicellular) red nucleus, nucleus of Darkschewitsch, zona incerta, nucleus of the posterior commissure, lateral intermediate layer and deep layer of the superior colliculus, dorsolateral periaqueductal gray, contralateral nucleus reticularis tegmenti pontis and basilar pontine nuclei (especially dorsal and peduncular), and dorsal (DAO) and medial (MAO) accessory olivary nuclei, ipsilateral lateral (external) cuneate nucleus (LCN) and lateral reticular nucleus (LRN), and to a lesser extent the caudal medial vestibular nucleus (MVN) and caudal nucleus prepositus hypoglossi (NPH), and dorsal medullary raphe.
(13) In contrast to this, adrenalectomy decreased ANP levels markedly in the organum vasculosum laminae terminalis and preoptic periventricular nucleus, which are reportedly involved in the central regulation of salt and water homeostasis.
(14) The shape of the nucleus changes from ovoid to a distinctive, radially splayed lobulated structure.
(15) Immunocytochemical analysis also suggested that bFGF immunoreactivity was present in the nucleus as well as the cytoplasm of astrocytes and CA2 neurons.
(16) The effect of Y-516 on the hyperactivity induced by dopamine injected bilaterally into the nucleus accumbens was compared with those of clocapramine (CCP), haloperidol (HPD) and chlorpromazine (CPZ).
(17) Moderately differentiated tumor revealed a wider range of nucleus size, less clustering (coefficient--3.59) and more hyperchromatic (70.1%) and "bare" (49.4%) nuclei and large nucleoli (22.2%).
(18) The majority of intensively stained and densely packed cells have been observed in tv nucleus.
(19) Following electrical stimulation of the hypothalamic paraventricular nucleus (PVN) area, 21% of the neurons were orthodromically excited, 6% were inhibited and 2.5% were antidromically activated.
(20) Proenkephalin A-related immunoreactive neuronal perikarya were detected in the central gray, reticular formation, nucleus raphes, trapezoid body, nucleus parabrachialis lateralis and medialis, nucleus spinalis nervi trigemini, nucleus dorsalis nervi vagi, and in the nucleus tractus solitarii.