(1) When present on a multicopy plasmid, this gene suppresses the phenotype of a number of amber mutants, but has no effect on the ocher mutants tested.
(2) In this system, (i) the natural N-terminal coding region of the hIL-6 gene was replaced by a synthetic sequence containing A-T rich codons, (ii) dual Shine-Dalgarno (SD) sequences were employed, (iii) an A-T rich segment was inserted in front of the initiation codon to avoid putative mRNA secondary structure in the region and (iv) the natural amber termination codon of the hIL-6 gene was changed to an ocher stop codon.
(3) I looked up the definition for redskin and although most online dictionaries agree that the term is offensive, most will give the definition as: "The original name was a European one used to describe Algonquins who painted their face with bright red ocher and bloodroot, thereby making their face red with war paint."
(4) The oligonucleotides synthesized have a sequence corresponding to the minus strand that is complementary to the viral strand at the start of gene G in bacteriophage phi X174 replicative form DNA where the normal third codon has been replaced with the ocher codon, TAA.
Ochre
Definition:
(n.) A impure earthy ore of iron or a ferruginous clay, usually red (hematite) or yellow (limonite), -- used as a pigment in making paints, etc. The name is also applied to clays of other colors.
(n.) A metallic oxide occurring in earthy form; as, tungstic ocher or tungstite.
(n.) See Ocher.
Example Sentences:
(1) Allelic complementation was not observed, despite testing of a large number of allele pairs, and alleles suppressible by the ochre suppressor SUP11 were absent from a sample of 48 spontaneous mutants and occurred infrequently (7%) among a sample of ultraviolet-induced mutants.
(2) Amber (UAG) and ochre (UAA) mutations are suppressed whereas UGA is not suppressed.
(3) Introduction of an ochre nonsense codon into the reading frame of the leader peptide sequence leads to considerable reduction of the basal expression and loss of inducibility of the cat gene.
(4) The first position of anticodon is 2'-O-methyluridine (Um), forming UmUA as the anticodon, which presumably recognizes the ochre termination codon UAA.
(5) Oligonucleotide-directed mutagenesis was used to generate amber, ochre and opal suppressors from cloned Arabidopsis and Nicotiana tRNA(Tyr) genes.
(6) The activities are present in yeast mutants which have greatly reduced levels of the three major vacuole-associated proteases (A, B and C) or which carry an ochre mutation in the major neutral protease (B).
(7) Mutants containing an ochre mutation in any essential yeast gene give rise to nonsectoring, white colonies, since cell growth is dependent on the presence of the plasmid-borne suppressor.
(8) Amber and ochre suppressor mutations in Salmonella typhimurium were selected.
(9) The nonsense mutants of S. pombe have been classified according to their suppressibility by defined opal and ochre suppressors into a class of efficiently suppressed opal and a class of inefficiency suppressed ochre mutants.
(10) Patterns of reversion produced by ciprofloxacin, enoxacin and ofloxacin in Salmonella typhimurium strains carrying the hisG428 ochre mutation have been studied.
(11) These replacements are consistent with a chain-terminating codon in am(17) of either the amber (UAG) or the ochre type (UAA), but are inconsistent with UGA.
(12) Allosuppressor (sal) mutations enhance the efficiency of the yeast ochre suppressor SUQ5 and define five unlinked loci, SAL1-SAL5.
(13) Using site-specific mutagenesis, we constructed five more efficient variants of tRNA(Glu)-Suoc, an extremely inefficient ochre suppressor.
(14) The opal suppressor form shows moderate suppressor activity when the gene is introduced on this vector, however, the ochre suppressor form exhibits no detectable biological activity regardless of gene copy number.
(15) 4-nitroquinoline-1-oxide (NQO) induces high frequencies of intragenic revertants of amber (UAG) but not ochre (UAA) mutants of yeast.
(16) Genetic recombination was assayed by measuring beta-galactosidase produced after recombination between two noncomplementing lacZ ochre alleles.
(17) The growth patterns of nonsense mutants of RNA (GA and f2) and DNA (lambda and T4) phages suggested that KO1 carried an amber, but not ochre or opal suppressors.
(18) Of 313 motility-deficient mutants isolated from an LT2 his(amber) strain fixed in phase 1 by gene vh2(-), 25 regained motility when amber or ochre suppressors were introduced, in F' factors or by transduction.
(19) The plasmid also increased frequency of UV-induced reversion to His+ in all tested his point mutants (wild type for UV sensitivity), including amber, ochre, UGA, missense, and frame-shift mutants.
(20) The results demonstrate that an ochre suppressor mutant of Escherichia coli K-12 produces abnormal 30S ribosomes.