(1) In contrast, inhibition of microtubule reassembly did not alter either normal deformability or the ZAP-induced decrease in deformability.
(2) It can be dissociated from the spores using divalent metal chelators and will reassemble on the spores in the presence of calcium.
(3) The mechanism of this inhibition is unknown, but reassembly experiments indicate that the 2 types of tubulins cannot copolymerize.
(4) Since the enzyme preferentially digests relaxed DNAs, these results suggest that nucleosomal subunits of c-myc and c-fos chromatin are relaxed during the state of active transcription, and reassembled once their transcription is repressed.
(5) The addition of Mg2+ to the dialyzing solution resulted in the formation of short intermediate-sized filaments even at 4 degrees C. Further dialysis of the short intermediate-sized filaments against reassembly solution containing both NaCl and MgCl2 at 37 degrees C failed to elongate them into longer filaments, suggesting that annealing does not contribute to the elongation of neurofilaments.
(6) Ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid treatment could also be used to extract RsaA and yielded protein capable of reassembly.
(7) We determined the time course for reassembly, the ultrastructural characteristics of reassembled NFs, and the topographical disposition of NF protein subdomains within reassembled NFs using quantitative biochemical techniques, negative staining and immunoelectron microscopy.
(8) Particles reassembled from homologous or heterologous mixtures of the RNAs and coat proteins of the viruses have the density of the nucleoprotein particles used as the source of protein.
(9) The specimens were reassembled, brushed with an Endobrush, and reevaluated.
(10) For the compounds investigated the spectra contain enough information to unequivocally reassemble the original sequence.
(11) Some of these regions have been identified by reassembly of the total tryptic peptides of apo B-100 with bovine brain sphingomyelin, 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC) and dimyristoylphosphatidylcholine (DPMC).
(12) The spinach small subunits were able to reassemble with the large subunit octamer of ribulosebisphosphate carboxylase-oxygenase from the cyanobacterium, Synechococcus ACMM 323, prepared as described previously (Andrews, T. J., and Ballment, B.
(13) The reassembled protein is also able to catalyze the oxidation of Fe(II).
(14) Although the 30S subunit was specifically destroyed by the heating process, both ribosomal particles were reassembled during recovery.
(15) It is suggested that the protein-associated lipid may be trapped between closely packed parallel aggregates of M13 coat protein and that the high local concentration of protein in a one-dimensional arrangement in lipid bilayers may be required for the fast reassembly of phage particles before release from an infected cell.
(16) The free segment reassembly fractured facial bones is but one additional technique gained from craniofacial surgery.
(17) In another case, PHA-receptor glycoproteins were purified by affinity chromatography and reassembled into PC-PS vesicles, using the same technique.
(18) The unfolding-refolding studies and role of substrate in reassembly were consistent with a mechanism involving at least two steps, possibly involving cis-trans isomerization of proline.
(19) The complexity of the structure of plasma high density lipoproteins (HDL) has invited numerous approaches which have been directed at the study of the intact particles, their apolipoproteins and reassembled complexes.
(20) Now the surgeon can reassemble the CEEA and perform the anastomosis.