(a.) Pertaining to, derived from, or containing, osmium; specifically, designating those compounds in which it has a valence higher than in other lower compounds; as, osmic oxide.
Example Sentences:
(1) The results suggest that the conversion of the HRP-TMB reaction product to an electron-dense form during osmication is intimately associated with the pH of the phosphate buffer and the total time of osmication.
(2) In agreement with previous findings osmicated cat 'C'-terminals failed to reveal synaptic complexes in regions possessing the subsynaptic cistern ('cisternal regions').
(3) The epitopes recognized by these mAbs were adversely affected by these fixatives; therefore, pre-embedding immunogold staining was employed, prior to fixation, osmication, dehydration and embedding.
(4) Aldehyde-fixed tissues are cut into sections with an automatic sectioner, incubated cytochemically osmication if required, then embedded in epoxy resin between fluorocarbon coverglasses which are supported by a platform specially designed for this purpose.
(5) The only complaint from the patient, months after the osmic acid injection, was a strong metallic taste on the tongue.
(6) They were studied with the prolonged osmication technique.
(7) With the polygalacturonase--gold complex, differences in the labelling distribution were noted according to the fixation procedure indicating, therefore, that osmication of the tissues could greatly interfere with the localization of the specific enzyme binding sites.
(8) We speculate that either oxidative polymerization of DAPA or Schiff's base formation with protein after aldehyde formation is responsible for the latter reaction, which is made permanent for ultracytochemical localization by osmication at a later step.
(9) This model has been tested by tracing the profiles of vesicles within the PTA network and comparing their size and shape frequency distributions with those of osmicated synaptic vesicles.
(10) The lingual gingival and the alveolar mucosa of mandible of the house musk shrew (Suncus murinus) were stained by methylene blue vital staining or osmic acid staining, and mounted as whole thickness preparations.
(11) Various methods of dissection, fixation, osmication, sectioning and staining were tested in order to develop an acceptable technique for preparing 9, 10, 11, and 12-day-old rat otocysts for electron microscopic study.
(12) Eleven patients had never received osmic acid injections in this particular joint, whereas 18 had had such injections 1-24 months prior to surgery.
(13) The inferior medullary velum and tela choroidea were removed intact from the fourth ventricle, post-osmicated, dehydrated, critical point dried, coated with palladium-gold and examined in a Cambridge Stereoscan S4 scanning electron microscope.
(14) Thin 100-nm sections were then cut and stained from the osmicated blocks for ultrastructural studies.
(15) A category of large boutons forming synapses with the soma and proximal dendrites of spinal motoneurons was studied in glutaraldehyde-fixed, non-osmicated tissue stained with uranyl acetate and lead citrate.
(16) The "sebum" secretion was evaluated in caucasian, black, yellow and mullato people of different ages by the osmic acid technique utilizing a semi-quantitative scale of score ranging from 0 to 9 points.
(17) After 18 hours of growth on selective serum-agar medium C diphtheriae cultures with different toxicogenic activity, and also diphtheroid and Hoffmann's baccillus cultures were removed, washed of the remnants of the nutrient medium and fixed under cold conditions by two combined methods (with glutaric aldehyde-osmic acid--uranyl acetate, and potassium permeanganate--uranyl acetate).
(18) The organization of the granule is somewhat altered when osmication is omitted from the fixation procedure.
(19) Sequential treatments of osmicated specimens with tannic acid pH 7.0-9.0, and uranyl acetate, pH 4.1, enhanced the density of the elastin intensely, increased collagen staining moderately, but hardly increased the density of nuclei and microfibrils.
(20) The reaction product even at this stage prior to osmication is highly visible.
Osmium
Definition:
(n.) A rare metallic element of the platinum group, found native as an alloy in platinum ore, and in iridosmine. It is a hard, infusible, bluish or grayish white metal, and the heaviest substance known. Its tetroxide is used in histological experiments to stain tissues. Symbol Os. Atomic weight 191.1. Specific gravity 22.477.
Example Sentences:
(1) Effects of fixation with glutaraldehyde (GA), glutaraldehyde-osmium tetroxide (GA-OsO(4)), and osmium tetroxide (OsO(4)) on ion and ATP content, cell volume, vital dye staining, and stability to mechanical and thermal stress were studied in Ehrlich ascites tumor cells (EATC).
(2) The modified osmium-urea method gave better results and was easier to perform than the collagenase method.
(3) Additionally, by ultrasonic vibration of tissues that had been subjected to prolonged osmium fixation, the epithelium was removed and such microdissected membranes similarly were examined.
(4) The renal tissue was fixed with a mixture of buffered picric acid-paraformaldehyde-glutaraldehyde and immunostained with the peroxidase-antiperoxidase method for the electron microscope with the following steps: antikallikrein antiserum, anti-IgG serum, peroxidase-antiperoxidase complex, 3-3' diaminobenzidine-H2O2, and post-staining with osmium tetroxide.
(5) It was also found that tritium release also occurred, although to a lesser extent, when tissues were fixed in glutaraldehyde or osmium tetroxide using collidine as a buffer, and this release was not significant when collidine was applied to previously fixed tissues.
(6) In accordance with the physiological experiments, electronopaque pyroantimonate precipitate containing calcium was found in the lumina of caveolae, but not in any intracellular structures close to the plasma membrane, when the relaxed fibers were fixed in a 1% osmium tetroxide solution containing 2% potassium pyroantimonate.
(7) The addition of oxalate to a suspension of rabbit peritoneal neutrophils before fixation with glutaraldehyde and postfixation with osmium tetroxide-antimonate greatly enhanced the amount of calcium antimonate precipitate subsequently detectable with the electron microscope.
(8) Ultrastructurally, hemolytic concentrations of tributyltin can be visualized in the electron microscope by osmium staining during fixation as electron-dense spheres penetrating the lipid bilayer of the erythrocyte plasma membrane.
(9) Their visualisation appeared to be enhanced when ruthenium red was incorporated n the glutaraldehyde-osmium fixative but only when sections were stained with heavy metal salts, indicating that the fimbriae and capsule were not predominantly polysaccharide in nature.
(10) Intravascular fat in tissue postfixed in osmium tetroxide, embedded in epoxy or paraffin, and stained with toluidine blue, hematoxylin, or Oil Red O is more easily visualized than in frozen tissue that is stained with Oil Red O.
(11) Mitotic cells were selectively removed from the cultures with 0.2 per cent trypsin and fixed in either osmium tetroxide or glutaraldehyde followed by osmium tetroxide.
(12) Human cochleae were fixed in glutaraldehyde, without the use of osmium.
(13) No reaction was observed in general with osmium-amine, with the exception of a few cases.
(14) Glutaraldehyde-fixed testes were stained "en bloc" with the Ur-Pb-Cu technique of Thiéry and Rambourg ('76) or post-fixed and stained with the osmium tetroxide-potassium ferrocyanide method of Karnovsky ('71).
(15) Insertion of 28 alternating AT residues at the EcoRV site of pBR322 generates an site hypersensitive to osmium tetroxide modification, that does not serve as a transcription start site.
(16) The osmium tetroxide fixation technique was used for determination of cell size and number.
(17) It uses carbohydrazide as an osmium bridging agent and both osmium tetroxide and uranyl acetate as electron staining agents.
(18) Parallel microsamples were fixed with osmium tetroxide and with glutaraldehyde followed by osmium tetroxide as previously used in this laboratory for the preservation of energy-linked mitochondrial configurations.
(19) A combination of 2% osmium tetroxide-2% uranyl acetate or 2% gallic acid alone resulted in optimum fixation as ascertained by least extraction of radiolabels.
(20) In those instances, the osmium-amine reacted not only in the outer surface of the mesentery, but also penetrated with no visible reaction all the way to the connective tissue where it was detected in the elastic layer.