(1) The affinity system used was the immobilized oxamate derivative previously used to purify mammalian lactate dehydrogenases.
(2) The most interesting derivative of the new series was N-[4-(3-methyl-5-isoxazolyl)-2-thiazolyl]oxamic acid 2-ethoxyethyl ester (49), which was also active and more potent than DSCG in experimental models involving either IgE- or IgG-mediated anaphylactic responses at bronchopulmonary level.
(3) N-(4-Nitrophenyl)oxamic acid[1] (1) was coupled with Sepharose 4B containing 1,6-diaminohexane as spacer group.
(4) Oxamate added to the suspension was ineffective in modifying this activity.
(5) The presence of conjugated N-(2-hydroxyethyl)-oxamic acid in some samples was suggested by the detection of small additional amounts of the free acid after treatment of the urine with beta-glucuronidase.
(6) Oxamate, a specific inhibitor of lactate dehydrogenase, prevented the inhibitory effect of D-lactate.
(7) Preincubation of the vesicles with oxamate or p-chloromercuribenzoate inhibited Rb+ uptake, but their addition to respiring vesicles again did not cause efflux.
(8) Those ovariectomized rats receiving replacement therapy were also resistant to oxamate effects.
(9) The oxamate derivative attenuates the increase in number of eosinophils and mononuclear cells obtained in the bronchoalveolar lavage fluid 24 hr after an active anaphylactic shock induced by aerosol.
(10) The HCO3(-)-dependent MgATP cleavage is also sensitive to inhibition by a pyruvate carboxylase inhibitor, oxamate, and the dependence of the reaction on the free Mg2+ concentration is similar to that of the pyruvate-carboxylation reaction, whereas the HCO3(-)-independent MgATP cleavage is not dependent on the concentration of free Mg2+ in the range tested.
(11) Two neuraminidases were isolated from the culture medium and purified by various procedures of gel chromatography, ion exchange chromatography, and by affinity chromatography on N-(4-nitrophenyl)-oxamic acid-Sepharose 4B.
(12) Also synthesized and investigated were two (2-carbosy-1,4-dihydro-4-oxobenzo[h]-quinolyl)oxamic acids (9 and 10).
(13) N-(2-hydroxyethyl)-oxamic acid is formed when metronidazole is reduced either chemically or by the action of the intestinal bacteria.
(14) In castrate rats, however, the addition of oxamate to the medium significantly reduced oxytocin-driven and spontaneous motility.
(15) Nine heterocyclic oxamic acid derivatives were synthesized and tested in the rat passive cutaneous anaphylactic assay as potential antiallergy agents.
(16) Preliminary characterization of the liver-specific isozyme relative to the eye-specific LDH-C4 in the Basketmouth cichlid with respect to thermolability and NADH-induced binding to oxamate-sepharose columns suggests that the eye- and liver-specific LDH isozymes are biochemically quite distinct in this fish and that they are probably encoded by two distinct loci.
(17) Finally, published frontal affinity chromatographic data for the NADH-dependent elution of rabbit muscle lactate dehydrogenase from oxamate-Sepharose are reanalysed using these equations to demonstrate the characterization of a system reflecting the binding of a solute-ligand complex to an affinity matrix.
(18) Substitution of the Sar1 residue of sarmesin with N,N-dimethyl-Gly, N-ethyl-Gly, aminoisobutyric, (methylamino)isobutyric, aminocaproic, and oxamic acids gave analogues that had the following respective antagonist activities (pA2) in the rat isolated uterus assay: less than 6, 6.9, 5.5, 6.0, less than 6, and 5.3.
(19) The mechanism of activation of the lactate dehydrogenase from Streptococcus faecalis by fructose 1,6-bisphosphate was investigated by using the immobilized oxamate gel.
(20) The reduction in the magnitude of the 13C isotope effect for the oxamate-dependent decarboxylation of oxalacetate from 1.0238 to 1.0155 when the reaction was performed in D2O (primary deuterum isotope effect = 2.1) clearly indicates that the transfer of the proton and carboxyl group between biotin and pyruvate does not occur via a single concerted reaction.
Salt
Definition:
(n.) The chloride of sodium, a substance used for seasoning food, for the preservation of meat, etc. It is found native in the earth, and is also produced, by evaporation and crystallization, from sea water and other water impregnated with saline particles.
(n.) The neutral compound formed by the union of an acid and a base; thus, sulphuric acid and iron form the salt sulphate of iron or green vitriol.
(n.) Fig.: That which preserves from corruption or error; that which purifies; a corrective; an antiseptic; also, an allowance or deduction; as, his statements must be taken with a grain of salt.
(n.) Any mineral salt used as an aperient or cathartic, especially Epsom salts, Rochelle salt, or Glauber's salt.
(n.) Marshes flooded by the tide.
(n.) Of or relating to salt; abounding in, or containing, salt; prepared or preserved with, or tasting of, salt; salted; as, salt beef; salt water.
(n.) Overflowed with, or growing in, salt water; as, a salt marsh; salt grass.
(n.) Fig.: Bitter; sharp; pungent.
(n.) Fig.: Salacious; lecherous; lustful.
(v. t.) To sprinkle, impregnate, or season with salt; to preserve with salt or in brine; to supply with salt; as, to salt fish, beef, or pork; to salt cattle.
(v. t.) To fill with salt between the timbers and planks, as a ship, for the preservation of the timber.
(v. i.) To deposit salt as a saline solution; as, the brine begins to salt.
(n.) The act of leaping or jumping; a leap.
Example Sentences:
(1) Samples are hydrolyzed with Ba (OH)2, and the hydrolysate is passed through a Dowex-50 column to remove the salts and soluble carbohydrates.
(2) Ursodeoxycholate was the only dihydroxy bile salt which was able to solubilize phospholipid (although not cholesterol) below the critical micellar concentration.
(3) Furthermore, recent investigations into the pharmacokinetics of lithium salts are dealt with.
(4) The influence of calcium ions on the electrophoretic properties of phospholipid stabilized emulsions containing various quantities of the sodium salts of oleic acid (SO), phosphatidic acid (SPA), phosphatidylinositol (SPI), and phosphatidylserine (SPS) was examined.
(5) The role of adrenergic agents in augmenting proximal tubular salt and water flux, was studied in a preparation of freshly isolated rabbit renal proximal tubular cells in suspension.
(6) An investigation of the constitutive ions of salts revealed that their effects were additive only in the case of salts that have no specific binding capability.
(7) Benzyloxycarbonylarginine p-nitrophenyl ester and other activated esters of N-a-sustituted arginine salts may be useful reagents for introduction of trypsin-labile protecting groups into peptide fragments for purpose of polypeptide semi-synthesis.
(8) The association constants K'A, KN, and K'N in the scheme (see article), were determined for the magnesium salts of ADP, adenyl-5'-yl imidodiphosphate AMP-P(NH)P, and PPi.
(9) In contrast to this, adrenalectomy decreased ANP levels markedly in the organum vasculosum laminae terminalis and preoptic periventricular nucleus, which are reportedly involved in the central regulation of salt and water homeostasis.
(10) For routine use, 50 mul of 12% BTV SRBC, 0.1 ml of a spleen cell suspension, and 0.5 ml of 0.5% agarose in a balanced salt solution were mixed and plated on a microscope slide precoated with 0.1% aqueous agarose.
(11) Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo.
(12) Mixed micelles of bile salt and phospholipids inhibit the lipase-colipase-catalysed hydrolysis of triacylglycerols.
(13) The first one is a region with iodine insufficiency; the second one is a region where the people use table salt in excess.
(14) One cellulase is buffer-soluble, the other buffer-insoluble but extractable with high salt concentrations.
(15) If salt fluoridation could also be generalized, caries levels could be reduced to a fraction of their initial values.
(16) The major lipase in human milk is dependent on bile salts for activity and probably participates in intestinal digestion of milk lipids in the newborn.
(17) The strain was resistant to bile salts in TCBS medium and demonstrated several properties from a borderline of two Vibrio and Aeromonas species.
(18) Sodium taurolithocholate, a monohydroxy bile salt, does not affect the CD spectrum of CEase, and neither the di- or the monohydroxy bile salt activates the enzyme.
(19) It is therefore suggested that salt water adaptation triggers a cellular reorganization of the epithelium in such a way that leaky junctions (a low resistance pathway) appear at the apex of the chloride cells.
(20) Depending on the differential sensitivity of nuclear T-ag to extraction by salt and detergent, nuclear T-ag could be separated into nucleoplasmic T-ag, salt-sensitive T-ag and matrix-bound T-ag subclasses.