(1) To study the role of the PENK gene in opioid related processes, an antisense sequence of PENK gene was subcloned into pSVL SV 40 late promoter expression vector and stably transfected into NG 108-15 cells, which contain opioid receptors.
(2) From steady-state kinetics half-life estimates of 9.6 h for PPenk mRNA and 14.7 h for Penk peptide were obtained.
(3) The Penk Combat Exposure scale did not separate the groups.
(4) "I was half expecting to see him on Songs of Praise," Penk added.
(5) Clinical observations and empirical evidence suggest that, among Vietnam combat veterans, Blacks are more maladjusted than Whites (e.g., Parsons, 1985; Penk et al., 1985).
(6) Penk, who now owns the Oldham-based local radio station 96.2 Revolution, said he was "appalled" at the "sheer scale of this unfair cross-promotion".
(7) "In these hard times it is difficult enough for the large, quasi-national commercial radio brands to compete with such unfair 'free' publicity," said Penk.
(8) The Penk Combat Intensity scale, which was correlated highly with the Keane scale, differentiated the PTSD-positive patients from both the normals and the PTSD-negative patients, but with less differentiating power (omega 2'S = .07 and .08).
(9) Facebook Twitter Pinterest The former Capital and Virgin Radio DJ Steve Penk has written to the BBC Trust to complain about the "excessive" and "gratuitous" cross-promotion of Chris Evans's new Radio 2 breakfast show on television.
(10) An increase in PENK mRNA levels has been also observed in cultures treated with 8-Br-cAMP, phorbol 12-myristate-13-acetate (TPA), or dexamethasone.
(11) Thus, activation of Penk gene expression upon neurotransmitter challenge is suggested to be due to an enhanced transcriptional activity of the gene mediated by de novo synthesized protein (-like) factors.
(12) We suggest that both the increased PENK message and the unchanged levels of free Met-enkephalin may be the result of compensatory mechanisms induced by translational inhibition by antisense, although the underlying processes remain to be determined.
(13) Penk was also critical of what he called the "placement" of Evans on BBC TV shows including Friday Night with Jonathan Ross, the One Show and the Andrew Marr Show.
(14) Unilateral adrenal denervation resulted in a rapid decrease in PPenk mRNA levels of 54% after 2 days, and by 11 days 90% of Penk mRNA had disappeared.
(15) Penk claimed the Evans trail broke the BBC Trust's own policy on cross-promotion.
(16) However, incubation of the cultures with the opioid agonist etorphine or the antagonist naltrexone did not alter PENK gene expression, suggesting that there is not feedback control of opioids on PENK biosynthesis in these cells.
(17) In this study we identified the potential sites of synthesis by localizing the mRNA coding for PENK in the rat pituitary gland using in situ hybridization histochemistry.
(18) Depolarization with potassium chloride (KCl) or veratridine increases the expression of PENK mRNA in a time-dependent fashion, with a maximal increase of sixfold.
(19) The proenkephalin A (PENK) gene codes for several opioid peptides, including Met-enkephalin, an endogenous ligand of opioid receptors.
(20) After an initial lag phase (with no major alterations) Penk mRNA increased markedly between 6 and 12 h followed by a slower, steady increase up to 48 h. The nicotinic receptor antagonist tubocurarine (4 x 10(-7) M) and the Ca2+ channel blocker D600 (10(-5) M) prevent the subsequent rise of Penk mRNA levels after challenge with nicotine, when given within the lag phase (0-6 h), suggesting the need of continuous receptor occupation and Ca2+ entry for induction of gene expression.
Punk
Definition:
(n.) Wood so decayed as to be dry, crumbly, and useful for tinder; touchwood.
(n.) A fungus (Polyporus fomentarius, etc.) sometimes dried for tinder; agaric.
(n.) An artificial tinder. See Amadou, and Spunk.
(n.) A prostitute; a strumpet.
Example Sentences:
(1) It may have been like punk never ‘appened, but you caught a whiff of the movement’s scorched earth puritanism in the mocking disdain with which Smash Hits addressed rock-star hedonism.
(2) Tolokonnikova was given a two-year sentence for her part in Pussy Riot's "punk prayer" in Moscow's largest cathedral, calling on the Virgin Mary to "kick out Putin".
(3) Although she's been performing since 2000 – in the punk-cabaret duo the Dresden Dolls , in a controversial conjoined-twin mime act called Evelyn Evelyn (they wear a specially constructed two-person dress and have been castigated by disability groups for presenting conjoined twins as circus freaks, an accusation she denies) – in her new band, Amanda Palmer And The Grand Theft Orchestra , she's suddenly become a kind of phenomenon.
(4) Three members of the Russian feminist punk band Pussy Riot are facing two years in a prison colony after they were found guilty of hooliganism motivated by religious hatred, in a case seen as the first salvo in Vladimir Putin's crackdown on opposition to his rule.
(5) The following week I bought the EP, expecting more of the same anarchic techno-punk.
(6) Album of the year: Random Access Memories - Daft Punk Daft Punk snatches record of the year from Macklemore's tiny fists.
(7) T-shirts were rush-printed overnight, showing his bald, burly head above the logo: "Hi, I'm Joe Plumber and Obama is a punk."
(8) Radio remained hostile to electronic dance music unless it had a conventional pop song structure and vocals (as with the Prodigy's punk-rave or Madonna's coopting of trance on Ray of Light ).
(9) Daft Punk will make their first televised performance since 2008 , playing with artists including Stevie Wonder and Get Lucky collaborators Pharrell Williams and Nile Rodgers.
(10) At Chapel-le-Frith in 1786, for instance, Wesley recorded a kind of punk festival riot: "The terror and confusion was inexpressible.
(11) He has classical roots in common with Michael Clark, the Royal Ballet prodigy turned punk choreographer.
(12) Then a punk rock club called the Echo hooked up with us.
(13) Yet, there is no doubt that All Star has been targeted for its specific qualities – the main ones being its feelgood nostalgia value and a laughably exuberant pop-punk style that feels totally earnest.
(14) Nile says: “The robots had an amazing vision!” Nile Rodgers accepts Daft Punk's International Group Award.
(15) A storm of criticism broke in Russia following the harsh two-year prison sentences given to three members of the feminist punk band Pussy Riot for protesting against the government in a Moscow cathedral.
(16) One of the most punk-rock things they ever did was dressing Chevy Chase up for his Ford impression: They didn’t do a damn thing.
(17) So, yes, Daft Punk are very famous indeed, but the two Frenchmen sitting side by side on a sofa in a luxurious Paris hotel suite – Thomas Bangalter, 38, and Guy-Manuel de Homem-Christo, 39 – are very much not.
(18) Track listing: What Goes Boom Greens and Blues Indie Cindy Bagboy Magdalena 318 Silver Snail Blue Eyed Hexe Ring the Bell Another Toe in the Ocean Andro Queen Snakes Jaime Bravo Track listing for Live in the USA (feat Lenchantin on bass): Bone Machine Hey Ana Magdalena 318 Snakes Indie Cindy I’ve Been Tired Head On The Sad Punk Distance Equals Rate Times Time Something Against You Isla de Encanta Planet of Sound Reading this on mobile?
(19) It was famous for being a big party house for local punks,” Clement says.
(20) Punks, poets, painters, dropouts, drug fiends, drag queens - all have been welcome at the Chelsea hotel.