(1) Soya bean trypsin inhibitor and phenylmethane sulphonyl fluoride (PMSF) had no effect on its activity.
(2) There was some evidence that only a single protease was present as the ratio of protease activity at various pH values did not alter significantly during purification or when the purified protease was partially heat-inactivated or treated with two specific trypsin-type protease inhibitors: N-alpha-tosyl-l-lysylchloromethyl ketone or phenylmethane-sulfonyl fluoride.
(3) The effects of chymase were abolished by enzyme inhibitor phenylmethane sulfonyl fluoride.
(4) The following five products were extracted with chloroform at pH 1--2: o-(chlorphenyl)-phenylmethane (I), benzophenone (II), o-chlorbenzophenone (III), benzhydrol (IV), and o-(chlorphenyl)-beta-phenylacrolein (V).
(5) Calcium protected higher molecular weight pituitary bFGFs from truncation by the endogenous activity, which was not acid-activated, co-purified with bFGF during heparin-sepharose chromatography, remained operant at high salt concentrations and was inhibited by phenylmethan-sulfonyl fluoride.
(6) Titration of the proteases with diisopropylfluorophosphate and phenylmethane sulfonylfuoride indicate equivalent weights of 28,500 and 32,800 g, respectively, for the proteins.
(7) Prolonged inhibition of the enzyme by phenyl N-benzyl-N-methylcarbamate or phenylmethane-sulphonyl fluoride does not lead to neurotoxic effects.
(8) antipain, pepstatin A, and phenylmethane sulfonyl fluoride) were shown to decrease the rate of incision DNA degradation and the level of unscheduled synthesis of DNA in Ehrlich ascites tumor cells exposed to UV-light and gamma-radiation.
(9) A protease inhibitor, phenylmethane sulfonylfluoride, was introduced in the solubilization and purification step.
(10) The fibrinogenolytic activity of alpha-fibrinogenase was markedly inhibited by EDTA and cysteine, while that of beta-fibrinogenase was inhibited markedly by phenylmethane sulfonylfluoride and slightly by tosyl-L-lysine chloromethylketone and cysteine.
(11) Also, the elution profile of phenylmethane-sulphonyl fluoride-inhibited thrombin from fibrinogen-Sepharose was identical with that of active thrombin from fibrin-monomer-Sepharose.
(12) Isolated form III was converted to forms II and I, and isolated form II was converted to form I by the coarse particulate fraction sedimenting at 1000 X g. This activity was inhibited by the serine enzyme inhibitor phenylmethane sulfonyl fluoride or by raising the pH to 8.7.
(13) As tested by the dietary route, the optimum effect among the phenylmethanes was reached with diphenylmethane, toluene, tri- and tetraphenylmethanes and the Sn analog, tin tetraphenyl being inactive.
(14) Penicillin acylase (EC 3.5.1.11) was completely inactivated with equimolar phenylmethane [35S]sulphonyl fluoride (PhMe35SO2F); the stability of the sulphonyl group in the modified protein was determined by measurement of the radioactivity in ultrafiltrates.
(15) Incubation of partially purified lipoprotein lipase for 24 h at 37 degrees C results in breakdown of the 55,000-dalton protein with concomitant enrichment in lower Mr components; the proteolytic activity is prevented by incubating the milk with phenylmethane, sulfonyl fluoride prior to chromatography on heparin-Sepharose.
(16) Treatments with organic solvents and heat were avoided and chromatographic and filtration techniques in the presence of phenylmethane sulfonyl fluoride were mainly used.
(17) It is not affected by phenylmethane sulfonylfluoride or soybean trypsin inhibitor, while it is completely inhibited by 0.5 mM EDTA or ethyleneglycol bis (beta-amino ethylether) N,N,N',N'-tetraacetic acid (EGTA).
(18) Circular dichroism was used to observe the active-inactive equilibrium in methan sulfonyl-alpha-chymotrypsin and phenylmethane sulfonyl-alpha-chymotrypsin.
(19) A growth-inhibitory zone was observed using 10 kinds of blue, purple, or green dyes, which were mainly phenylmethane dyes.
(20) The enzyme is strongly inhibited by inhibitors of serine proteinases, diisopropylfluorophosphate, phenylmethane-sulfonyl fluoride, m-aminobenzamidine, aprotinin, and leupeptin but not by inhibitors of either thiol-, metallo- or carboxyl-proteinases.
Toluene
Definition:
(n.) A hydrocarbon, C6H5.CH3, of the aromatic series, homologous with benzene, and obtained as a light mobile colorless liquid, by distilling tolu balsam, coal tar, etc.; -- called also methyl benzene, phenyl methane, etc.
Example Sentences:
(1) The specific rates of degradation of L-arginine-AMC, gly-proline-AMC, N-alpha-benzoyl-L-arginine-AMC and N-[p-toluene-sulphonyl]gly-pro-arginine-AMC were significantly greater in that group, indicating that the composition of their gingival crevicular fluid was different from that of the gingivitis group.
(2) The toluene group were more approving in their attitudes towards taking other drugs.
(3) In anaerobiosis, at 25 mM sulphanilic acid, or with addition of p-toluene sulphonic acid only one regression line is obtained for the permeation in both directions.
(4) Their defect in DNA degradation was shown not only after treatment by toluene but also in crude extracts after cell disintegration by ultrasonic and in untreated starved cultures.
(5) The significance of the present findings on the mutual suppression of metabolism between benzene and toluene is discussed in relation to solvent toxicology and biological monitoring of exposure to the solvents.
(6) On the other hand, with simultaneous doses of the two substances, the blood toluene concentration was higher for the first 15-30 min than the ethanol control and the urinary excretion of hippuric acid, a main metabolite of toluene, was markedly decreased for the first 2 h. The blood ethanol in this group, on the contrary, was reduced until 1 h after administration.
(7) Toluene exposure for 4 and 12 weeks caused a significant, approximately 20%, increase in 45Ca2+ uptake into unstimulated synaptosomes.
(8) Furthermore, toluene undergoes methyl-substitution in preparations of human bone marrow incubated with S-adenosyl-L-methionine to yield o-xylene, m-xylene, and p-xylene.
(9) We have investigated the whole-body dermal penetration of styrene, xylene, toluene, perchloroethylene, benzene, halothane, hexane, and isoflurane in rats and compared the permeability constants with available human studies on vapor penetration.
(10) The metabolites were identified as a) 3-hydroxy-2,6-DAT, b) 4-hydroxy-2-acetylamino-6-aminotoluene, c) 2-acetylamino-6-aminotoluene, and d) 2,6-di(acetylamino)-toluene.
(11) Thus chronic toluene inhalation even at small concentrations changes hypothalamic reactivity in response to stress and hormonal stimuli.
(12) The coefficient to the complex action of carbon oxide and heating microclimate is equal to 2.5 and toluene++ and heating microclimate to 1.9.
(13) Expired carbon dioxide (CO2), the most sensitive index, displayed an inverted U-shaped concentration-effect curve, which increased at 100 ppm (the TLV) and decreased at 4500 ppm toluene.
(14) The paint base consisted primarily of toluene and methyl ethyl ketone.
(15) As organic solvents, 1,1,1-trichloroethane (1,1,1-TCE), trichloroethylene, tetrachloroethylene, toluene, m-xylene and trichloro-trifluoroethane (FC-113) were used in this study.
(16) There was a negative association between blood toluene and plasma levels of prolactin.
(17) Ethanol-withdrawn animals displayed an increased sensitivity to the narcotic action of toluene.
(18) One possibility is that early synaptic alterations resulting from toluene exposure may be preceded by increases in outer membrane fluidity.
(19) In experiments in vitro, neither benzene, toluene nor xylene changed the number of sister-chromatid exchanges (SCEs) or the number of chromosomal aberrations in human lymphocytes.
(20) Benzene toluene, styrene, 1,1,1-trichloroethylene, and tetrachloroethylene are taken as examples of solvents showing various toxicokinetic properties.