(1) pIgG, independent of its effect on insulin binding, inhibited both basal and insulin-stimulated autophosphorylation of the insulin receptor in a dose-dependent manner with a half maximal effect at 3.3 to 7 micrograms protein.
(2) Moreover, these findings suggest that pIgG may inhibit autophosphorylation by acting on domains which are similar in the insulin and EGF receptors.
(3) The relative affinity of two mIgGs for human t-PA was found to be equal or even superior to that of pIgG.
(4) Furthermore, pIgG also reduced basal autophosphorylation of the EGF receptor.
(5) The immunoglobulin G of a polyclonal antiserum (pIgG) from a patient with insulin resistance and hypoglycemia was tested for its ability to inhibit insulin binding and to affect the autophosphorylation of partially-purified insulin receptors extracted from rat liver membranes.
(6) He seems tired of his tragic love life and can hardly bring himself to consummate an unsatisfying fling with his housing officer, Pamela Pigg.
(7) The effect of pIgG to inhibit basal autophosphorylation of insulin and EGF receptors, together with its ability to reduce autophosphorylation of insulin receptors fully occupied by insulin, imply that the effect of pIgG on receptor autophosphorylation is largely independent of its effect on ligand binding.
(8) A human polyclonal IgG (pIgG), previously characterized as anti insulin receptor antibody, able to inhibit insulin receptor kinase activity, was used to further investigate subunit homologies and differences in antigenicity and functional regulation between IGF I and insulin receptors, IGF I receptor tyrosine kinase was stimulated by a IGF I analog (aIGF I), produced by DNA recombinant technology, pIgG was able to inhibit IGF I receptor kinase activity, thus revealing antigenic homologies between the kinase domains of insulin and IGF I receptors.
(9) Using these products in DI together with antibodies against PIgG class- and subclass-specific determinants it was proven that at least two PIgG subclasses exist (tentatively called IgG1 and IgG2).
(10) Two of the fragments reacting with PIgG antisera remained intact even after 120 h digestion.
(11) pIgG, when added 4 hr prior to insulin, inhibited subsequent insulin binding by 50% at 30 micrograms added protein; however, insulin previously bound to the receptor could not be displaced by a 4 hr subsequent exposure of up to 70 micrograms pIgG.
(12) To investigate the existence of two or more porcine IgG (PIgG) subclasses PIgG was isolated from whey and serum by precipitation with caprylic acid followed by ion-exchange chromatography.
(13) Waugh's treatment of Pappenhacker is significant, because unlike some of the stock journalistic characters in Scoop – Shumble, Pigge, Whelper, even the capable Corker – he is treated as zealous in the pursuit of truth.
(14) However the more pronounced inhibition of IGF I receptor-compared with insulin receptor kinase activity by pIgG suggests the existence of different regulatory mechanisms.
(15) Coelomic fluids of E.F. digest effectively vertebrate serum proteins (PIgG, HSA) but not the proteins of L.T.
(16) Soon after, the jury was told, Floorgraphics began to lose crucial contracts with key clients – Safeway, Winn-Dixie, the South Carolina retail chain Piggly Wiggly and others – many of whom defected to News America.
(17) Six monoclonal antibodies (mIgG) and a polyclonal antibody (pIgG) directed against human tissue-type plasminogen activator (t-PA) were tested for their species specificity towards human or murine t-PA.
(18) A minimal coupling stoichiometry of 2.2 palmitic acids per IgG was essential for the stabilization activity of pIgG.
(19) In addition, the minimal pIgG to PE molar ratio for stable liposomes was 2.5 X 10(-4).
(20) Whereas pIgG as well as several mIgGs discriminated poorly between these two t-PA species, one mIgG (clone E3) was highly specific for human t-PA. Inhibition and binding studies of human t-PA by mIgGs revealed high affinity-high inhibitory (E3) as well as high affinity-poor inhibitory (B1) mIgGs.
Piggin
Definition:
(n.) A small wooden pail or tub with an upright stave for a handle, -- often used as a dipper.