(1) Ultrastructural studies of Aeromonas hydrophila strain AH26 revealed two distinctive pilus types: "straight" pili appear as brittle, rod-like filaments, whereas "flexible" pili are supple and curvilinear.
(2) Phagocytic killing in the presence of each monoclonal antibody paralleled the increase in chemiluminescence, suggesting that for this variant killing was an inevitable consequence of the interaction of polymorphonuclear leukocytes with gonococci opsonized with anti-pilus antibodies.
(3) These results show for the first time the role of a specific pilus structure in colonization of the human intestine by V. cholerae O1 and exemplify the significance of a genetic regulon in pathogenesis.
(4) The relative affinity of these peptides for anti-EDP208 pilus antibodies was determined by a competitive enzyme-linked immunosorbent assay using the Fab fragment of anti-EDP208 pilus immunoglobulin G. From these results we established that the antigenic region of this peptide was the N-terminal pentapeptide, N-acetyl-Thr-Asp-Leu-Leu-Ala, and the key residues responsible for the antibody-antigen interaction are the N-acetyl-Thr1, Leu3, and Leu4.
(5) The antigenic surface-associated colonization factor of E. coli strain H-10407 has been further characterized: this pilus-like antigen is produced under conditions of growth that repress the production of common pili of E. coli.
(6) The degree of S-pilin processing and the levels of membrane-associated pilin varied among the different classes of mutants, suggesting that each was blocked at a distinct step of pilus biogenesis.
(7) At the basis of each pilus, a cell wall differentiation was observed appearing, in face-on-view, as a ring-like structure made up of subunits, and in side-on view as a hollow cylinder penetrating through the cell wall.
(8) Using Western blot analysis of the immunizing pilus and its cyanogen bromide (CNBr) fragments, IgG antibody to pilin was detected before immunization in all individuals.
(9) K99+, 987P+, and type 1 pilus+ bacteria could be prevented from adhering to epithelial cells by Fab fragments specific for K99, 987P, or type 1 pili, respectively.
(10) One recombinant pilus was shown to elicit antibodies against the synthetic peptide in immunized rats.
(11) A purified Gal-Gal pilus vaccine prevented (P less than 0.05) subsequent colonization by a challenge wild-type strain that exhibited homologous pili.
(12) These results showed that trbC function is essential to the F plasmid conjugative transfer system and suggested that the TrbC protein participates in F-pilus assembly.
(13) These observations may indicate a possible evolutionary relationship(s) of plasmids unrelated by the criteria of incompatibility, pilus phage specificity, or plasmid host range.
(14) The presence of an invertible element (Min) is suggested which enables the formation of conjugative pili at 30 degrees C, but switches off the pilus formation at 37 degrees C incubation temperature.
(15) Three classes of pili, alpha, beta and gamma of ascending sub-unit size were identified among the 7 pilus antigen serogroups.
(16) A high titre of pilus antibodies was obtained by immunizing rabbits with mutants whose pili had lost their ability to retract into the cell.
(17) Earlier studies have shown that the majority of Escherichia coli isolates from urinary tract infections which possess a D-mannose-resistant adhesin contain gene sequences homologous to the pap pilus gene sequences encoded on the recombinant plasmid pRHU845.
(18) Like R100, R62 prevented transfer, pilus formation, and surface exclusion and, therefore, probably inhibits expression of the transfer operon traA through traI.
(19) The pilS1 locus contains six tandem pilus gene copies linked by a 39 bp repeat sequence also present in the expression loci.
(20) This mutant no longer produced a 20.5-kDa protein (TcpA) that we show is the major subunit of a V. cholerae pilus.
Pious
Definition:
(a.) Of or pertaining to piety; exhibiting piety; reverential; dutiful; religious; devout; godly.
(a.) Practiced under the pretext of religion; prompted by mistaken piety; as, pious errors; pious frauds.
(2) It is of course important that migrants are not scapegoated; but such pious deceit from comfortable middle-class commentators can only provoke the unemployed, the low-paid and the homeless.
(3) Still, I like to believe that these small-scale ventures, too, make some contribution to a conversation without limits or proscriptions; the sine qua non of the sort of society that knows to keep the solemn and the pious at bay.
(4) Many Isis fighters are newly converted, newly pious ... these men have grown a beard in three months and they don’t give Islam time to be understood.” He is tired of having to defend his religion against bigots who take these instant Islamists to be the authentic representation of Islam.
(5) The president, Recep Tayyip Erdoğan, made it a vote about his “way”, and found himself rejected by a large group of “democrat” voters – and almost completely abandoned by his long-term allies: pious Kurds.
(6) In that same National season, he teamed with Simon Callow (as Face) and Josie Lawrence (as Doll Common) in a co-production by Bill Alexander for the Birmingham Rep of Ben Jonson’s trickstering, two-faced masterpiece The Alchemist ; he was a comically pious Subtle in sackcloth and sandals.
(7) Both harangued Brian from the outset calling it "a squalid little film" and "tenth rate"; no amount of measured argument on the Pythons part would dissuade the pious double act of their firmly held belief that Life of Brian mocked Christ.
(8) He's obviously a true believer in democracy – which sounds rather pious, but it's a fact.
(9) Tories are furious and bitter at being abandoned by the Lib Dems, whom they loathe anyway as a bunch of pious creeps.
(10) Those who claim that conversion or rejection of faith is punishable by death are effectively - and this ought to give their pious hearts pause for reflection - usurping powers reserved solely for God.
(11) Burns is, according to the poet Edwin Muir, "to the respectable, a decent man; to the Rabelaisian, bawdy; to the sentimentalist, sentimental; to the socialist, a revolutionary; to the nationalist, a patriot; to the religious, pious …" So no doubt, this January at the start of referendum year , even diehard unionists will be searching around for words of his that seem to support their position and, where they can extrapolate them, sprinkling them around with abandon to salt their haggis, neeps and tatties at Burns suppers the length and breadth of the land.
(12) We simply cannot wait in the pious hope that short-term-minded governments and enterprises will save us There is a clear answer to the question of each country’s reasonable share, based on a permissible quantum of emissions per capita that never threatens the perilous 2C mean temperature increase that would profoundly and irreversibly affect all life on earth.
(13) The reticent, pious, even priggish character was too alien, possibly repellant, for the writer and director of the 1999 film version, Patricia Rozema, who drew on Austen's letters to fabricate another creature altogether.
(14) Against this background it is very simple to make such pious and ill-considered statements as, “If they don’t want to go to jail, they shouldn’t break the law!” Facebook Twitter Pinterest ‘We represent only 2.4% of the Australian population yet account for more than 25% of the prison population.’ Photograph: AAP Against this background, it is very simple to impose policies for Indigenous Australians that do not signal any sense of belief in our humanity and own capacity to rise above the challenges we are confronted by.
(15) What we have too in Sister Cristina is the singing nun as a cultural idea: the pious, virginal creature emerging from behind strict convent walls to charm the world with the power of her voice.
(16) Early speculation suggested the twice-divorced businessman – who once cited the verse “two Corinthians” rather than the correct “second Corinthians” during his campaign and said he had never sought forgiveness for his sins – could not capture the vote of the pious.
(17) Like The Guard, Calvary is tartly, tightly scripted; unlike it, it's a pious piece of work, a serious investigation of expiation.
(18) This pious art lover could have a career in slapstick if she wants, for her comic destruction of a work of art bears comparison with Rowan Atkinson giving Whistler's Mother a badly drawn cartoon face in the film Bean .
(19) Sometimes we are not quite sure that this way of life is pious enough.
(20) "I saw Jonathan, who comes over as a very nice, humble, pious person," said Selby.