(1) Pokeweed mitogen-stimulated rat spleen cells were identified as a reliable source of rat burst-promoting activity (PBA), which permitted development of a reproducible assay for rat bone marrow erythroid burst-forming units (BFU-E).
(2) The number of peripheral blood lymphocytes (PBL) producing IgM (spontaneous and pokeweed mitogen (PWM) stimulated) at the end of a seven day culture period was similar in PBC patients and control subjects while the amount of IgM synthesized (spontaneous and PWM stimulated) during this period was significantly greater in the patient group, implying that the amount of IgM produced per B cell was increased in PBC.
(3) Besides various skin tests with the antigens candida, trichophyton, mumps, streptokinase-streptodornase, tuberculin, DNCB and KLH also in vitro experiments measuring the immunoglobulin- and complement concentrations, the antibody production to KLH, the lymphocyte transformation rate to PHA, Pokeweed, Con A, PPD were done nearly in all patients.
(4) We propose to call the pokeweed antiviral protein isolated from pokeweed cells PAP-C. 3.
(5) The mitogenic responses to phytohemagglutinin (PHA) and concanavalin A (Con A), but not pokeweed mitogen (PWM), were decreased significantly in cultures with isolated lymphocytes, while in whole blood cultures the responses to PHA and PWM but not to Con A were reduced.
(6) Three wk after in vivo immunization with PRP and TT, in vitro stimulation with pokeweed mitogen, Staphylococcus aureus Cowan 1 bacteria, or antigen induced anti-TT but not anti-PRP in vitro antibody secretion, although Epstein-Barr virus induced both.
(7) Lymphocyte blastogenesis in hairless descendants of Mexican hairless dogs was examined using the following mitogens: phytohemagglutinin (PHA-M), Concanavalin A (Con A), lipopolysaccharide (LPS) and pokeweed mitogen (PWM).
(8) Pokeweed mitogen activation was reduced 50% at optimal doses of mitogen whereas the response was unaffected at suboptimal doses.
(9) The cells were stimulated with various dilutions of phytohaemagglutinin, Con A or pokeweed mitogen.
(10) Lectins which agglutinated neutrophils, but not necessarily CRBC, such as phytohaemagglutinin (PHA-P), concanavalin A (Con A), soybean agglutinin (SBA), and Ricinus communis agglutinin (RCA), mediated cytotoxicity while lectins which did not cause agglutination, such as pokeweed mitogen (PWM), did not mediate cytotoxicity.
(11) Ribosome-inactivating proteins were found in high amounts in one line of cells of Phytolacca americana (pokeweed) cultured in vitro and, in less quantity, in lines of Saponaria officinalis (soapwort) and of Zea mays (corn) cells.
(12) Pokeweed mitogen-stimulated interleukin 2 production in lymphocytes from cirrhotic patients was significantly lower than that of the noncirrhotic patients.
(13) The highest 3H-thymidine incorporation in cultures of dog lymphocytes was observed at day 3, while in those of fox at day 2, incubated either at 37 degrees C or at 39 degrees C. Lymphocytes cultured at 39 degrees C incorporated more tritiated thymidine than did cells cultured at 37 degrees C. The stimulation index (SI) of dog peripheral blood lymphocytes to both mitogens concanavalin A (Con A) and leucoagglutinin (LA) was in a similar range, while pokeweed mitogen (PWM) showed a weaker but significant stimulatory action.
(14) Low doses of concanavalin A and staphylococcal enterotoxin B and large doses of pokeweed mitogen caused MMI in "inactivated serum" medium, but MMI was enhanced in fresh serum.
(15) Normal human T-lymphocytes can be induced to form colonies with a high cloning efficiency by seeding the cells directly in agar with normal human plasma and the lectins concanavalin A, phytohaemagglutinin, or pokeweed mitogen.
(16) PBM prepared from BoT4-depleted animals also had a significantly reduced ability to respond in vitro to the mitogens phytohemagglutinin, concanavalin A and pokeweed mitogen.
(17) Lymphocyte cultures were stimulated by the plant mitogens phytohemagglutinin (PHA) and pokeweed mitogen (PWM), both in the presence of dextran and after dextran had been removed from the medium.
(18) Increases were observed in the binding of four lectins to LGLs after IL-2 activation; Triticum vulgaris (wheat germ agglutinin), Phytolacca americana (pokeweed mitogen), Lycopersicon esculentum (tomato lectin), and Griffonia simplicifolia I-B4 (GSI-B4).
(19) The response to pokeweed mitogen, while always reduced, was generally less markedly affected than the response to the other two mitogens.
(20) In a longitudinal study on 13 adult female stumptailed macaques, Macaca speciosa, peripheral blood samples were analyzed hematologically and the in vitro response of the lymphocytes to the common mitogens concanavalin A, phytohemagglutinin and pokeweed mitogen were investigated.