(1) On the basis of genetic, phenotypic and physiological criteria, these mutants are divided into four groups: 1) the cytochrome aa3 and b deficient "poky" variants that are defective in mitochondrial ribosomes assembly, 2) the cytochrome aa3 deficient mutants, [mi-3] and [exn-5], that appear to have genetic lesions affecting a component of a regulatory system controlling cytochrome aa3 synthesis, 3) the cytochrome aa3 and b deficient "stopper" mutants with physiological lesions that probably affect mitochondrial protein synthesis, and 4) cni-3, a mutant that is constitutive for an inducible mitochondrial cyanide-insensitive oxidase in spite of having a normal cytochrome mediated electron-transport system.
(2) Cell respiration in wild type and poky was studied as part of a long-term investigation of cyanide-resistant respiration in Neurospora.
(3) Six independently isolated Group 1 extranuclear mutants, namely [exn-1], [exn-2], [exn-4-a1, [stp-b1], [SG-1] and [SG-3-A1, which have growth and cytochrome phenotypes similar to [poky] also were found to be deficient in small subunits of mitochondrial ribosomes.
(4) Seven nonallelic nuclear suppressors of [poky] result in increased concentrations of the mt small rRNA and pre-rRNAs, but do not restore the ability to synthesize small rRNAs having the correct 5' ends.
(5) Group I consists of 8 female-fertile variants with both poky-like growth and cytochrome defects.
(6) Two-dimensional gel electrophoresis was used to examine mit ribosomal proteins from [poky] and six additional extra-nuclear mutants with defects in the assembly of mit small subunits.
(7) Six nuclear suppressors of the (poky) cytoplasmic mutant (sup-1, sup-3, sup-4, sup-5, sup-10, sup-14) have been obtained in Neurospora crassa.
(8) However, the transmission of the [poky] mutation does not appear to be correlated with the transmission of either of these insertions or of other physical markers.
(9) Mitochondria from the cytochrome-deficient mutant poky carried out substrate-driven K+ uptake at reduced rates, but oligomycin-sensitive, ATP-driven K+ uptake at rates about 60% greater than those shown by wild-type mitochondria.
(10) But actually Chote's team is only about 20-strong and they operate from a rather poky office on Victoria Street.
(11) We have compared the adenosine triphosphatase (ATPase) activity of mitochondria prepared from wild-type Neurospora crassa and from poky, a maternally inherited mutant known to possess defective mitochondrial ribosomes and reduced amounts of cytochromes aa3 and b. poky contains two distinct forms of mitochondrial ATPase.
(12) Pokie Nation, set to air next week on the ABC, has been edited to amend the claim.
(13) In all these experiments, no difference was detected between wild type and poky in base sequence or methylation of either 19 S or 25 S RNA.
(14) The six suppressors are nonallelic, suppress the phenotypic effects of (stp-Bl) in addition to (poky), but have no effect on the phenotype expression of the (mi-3) cytoplasmic mutant.
(15) An analysis of the paramagnetic components present in mitochondria isolated from the poky mutant of Neurospora crassa is described.
(16) Orta filmed the death of Eric Garner , capturing the moment the 43-year-old was placed in a banned chokehold by an NYPD officer and uttered his last words: “I can’t breathe.” Lawyers Ken Perry and William Aronin sit in the poky downtown Manhattan apartment that serves as their office.
(17) The mitochondrial phenotype of [poky] and other extranuclear Neurospora mutants is known to predominate over that of wild type in heteroplasmons.
(18) They are not allowed to draw in court, so they must observe, make notes about someone’s characteristics and clothes, and then retreat to a poky press room where they sketch and colour at high speed.
(19) The residual small rRNAs in [poky] appear to be synthesized via the upstream promoter(s), but are missing 37-44 nucleotides from their 5' ends, indicating either that pre-rRNAs are processed abnormally or that abnormal 5' RNA ends are unstable.
(20) The ribosomal defect shows an extrachromosomal pattern of inheritance in crosses of poky with wild type.
Slot
Definition:
(n.) A broad, flat, wooden bar; a slat or sloat.
(n.) A bolt or bar for fastening a door.
(n.) A narrow depression, perforation, or aperture; esp., one for the reception of a piece fitting or sliding in it.
(v. t.) To shut with violence; to slam; as, to slot a door.
(n.) The track of a deer; hence, a track of any kind.
Example Sentences:
(1) • Gone Girl picked for opening slot at New York film festival • We predict how Venice, Toronto and Telluride will split the 2014 world premieres
(2) When Question Time was moved to an earlier 9pm slot in May during the MPs' expenses scandal, a panel including Martin Bell, Ben Bradshaw and William Hague had 3.7 million viewers and a 17% share.
(3) McCall and her ad director, Stuart Taylor, have also managed to offer 'page dominance' to all but the smallest potential advertisers, meaning that big ads will not be diluted down by having smaller slots alongside them.
(4) DNA is isolated from synchronized populations of G1 and S phase cells, it is slot-blotted at the same DNA concentration(s) for each population, and it is hybridized with 32P-labeled DNA probes that are specific to the regions of interest.
(5) The final episode of I Own Britain's Best Home drew 400,000 and 2% for Five in the same time slot.
(6) Experiments were performed to measure velocities in front of six slot hoods.
(7) The issue was first raised by BBC2 controller Janice Hadlow and brought to the attention of the then BBC Vision director Jana Bennett – number two to BBC director general Mark Thompson – after the sitcom, which was planned for a post-9pm watershed slot, was moved to pre-watershed.
(8) Leroy Sané, a substitute, slotted in seamlessly on his debut.
(9) Standard gels, 200 mm wide with 20 sample slots have also been used.
(10) Findley darts round him and slots him beneath the advancing Ricketts.
(11) 27 August, 8pm Will Self The nearest the book festival circuit has to a rock star has three slots.
(12) The gastric factors controlling abundance of mRNA encoding the important neuropeptide, gastrin releasing peptide (GRP) in rat stomach, were examined by Northern and slot blot analysis.
(13) A complete 0.018-inch slot straight-wire appliance was used to align the teeth, close lower spaces, and detail the occlusion.
(14) It seemed that a gust of wind had dislodged part of the screen’s moorings leaving the visiting Leicester party, who had to negotiate a new take-off slot for their post-match flight back to East Midlands, looking unimpressed when they ventured to the touchline.
(15) FISH results on primary tumors were concordant with slot blot results on amplification and with immunohistochemical detection of overexpression.
(16) Eggs of southern corn rootworm (Diabrotica undecimpunctata howardi Barber) were subjected to electromagnetic energy at 2.45 GHz in slotted waveguide applicators to determine ovicidal threshold levels.
(17) When incubated with alpha-32P-labeled ribonucleoside triphosphates in vitro, nuclei isolated from haploid or diploid cells transcribed rRNA, tRNA, and mRNAs in a strand-specific manner, as shown by slot blot hybridization of the in vitro synthesized RNA to cloned genes encoding 5.8S, 18S and 28S rRNAs, tRNATyr, and GAL7, URA3, TY1 and HIS3 mRNAs.
(18) Herein, we describe the procedures for preparation and labeling of DNA probes and the principles that regulate dot, slot and Southern blot hybridization.
(19) It was considerably — and predictably — up on the audience who used to watch Norton in his old 10pm slot on BBC2, when it was typically watched by between 1 million and 1.5 million viewers.
(20) BBC1 slipped to second place in the slot behind a repeat of Martin Clunes drama Doc Martin, with 3.6 million viewers and an 18% share between 9pm and 11pm.