(1) On the basis of genetic, phenotypic and physiological criteria, these mutants are divided into four groups: 1) the cytochrome aa3 and b deficient "poky" variants that are defective in mitochondrial ribosomes assembly, 2) the cytochrome aa3 deficient mutants, [mi-3] and [exn-5], that appear to have genetic lesions affecting a component of a regulatory system controlling cytochrome aa3 synthesis, 3) the cytochrome aa3 and b deficient "stopper" mutants with physiological lesions that probably affect mitochondrial protein synthesis, and 4) cni-3, a mutant that is constitutive for an inducible mitochondrial cyanide-insensitive oxidase in spite of having a normal cytochrome mediated electron-transport system.
(2) Cell respiration in wild type and poky was studied as part of a long-term investigation of cyanide-resistant respiration in Neurospora.
(3) Six independently isolated Group 1 extranuclear mutants, namely [exn-1], [exn-2], [exn-4-a1, [stp-b1], [SG-1] and [SG-3-A1, which have growth and cytochrome phenotypes similar to [poky] also were found to be deficient in small subunits of mitochondrial ribosomes.
(4) Seven nonallelic nuclear suppressors of [poky] result in increased concentrations of the mt small rRNA and pre-rRNAs, but do not restore the ability to synthesize small rRNAs having the correct 5' ends.
(5) Group I consists of 8 female-fertile variants with both poky-like growth and cytochrome defects.
(6) Two-dimensional gel electrophoresis was used to examine mit ribosomal proteins from [poky] and six additional extra-nuclear mutants with defects in the assembly of mit small subunits.
(7) Six nuclear suppressors of the (poky) cytoplasmic mutant (sup-1, sup-3, sup-4, sup-5, sup-10, sup-14) have been obtained in Neurospora crassa.
(8) However, the transmission of the [poky] mutation does not appear to be correlated with the transmission of either of these insertions or of other physical markers.
(9) Mitochondria from the cytochrome-deficient mutant poky carried out substrate-driven K+ uptake at reduced rates, but oligomycin-sensitive, ATP-driven K+ uptake at rates about 60% greater than those shown by wild-type mitochondria.
(10) But actually Chote's team is only about 20-strong and they operate from a rather poky office on Victoria Street.
(11) We have compared the adenosine triphosphatase (ATPase) activity of mitochondria prepared from wild-type Neurospora crassa and from poky, a maternally inherited mutant known to possess defective mitochondrial ribosomes and reduced amounts of cytochromes aa3 and b. poky contains two distinct forms of mitochondrial ATPase.
(12) Pokie Nation, set to air next week on the ABC, has been edited to amend the claim.
(13) In all these experiments, no difference was detected between wild type and poky in base sequence or methylation of either 19 S or 25 S RNA.
(14) The six suppressors are nonallelic, suppress the phenotypic effects of (stp-Bl) in addition to (poky), but have no effect on the phenotype expression of the (mi-3) cytoplasmic mutant.
(15) An analysis of the paramagnetic components present in mitochondria isolated from the poky mutant of Neurospora crassa is described.
(16) Orta filmed the death of Eric Garner , capturing the moment the 43-year-old was placed in a banned chokehold by an NYPD officer and uttered his last words: “I can’t breathe.” Lawyers Ken Perry and William Aronin sit in the poky downtown Manhattan apartment that serves as their office.
(17) The mitochondrial phenotype of [poky] and other extranuclear Neurospora mutants is known to predominate over that of wild type in heteroplasmons.
(18) They are not allowed to draw in court, so they must observe, make notes about someone’s characteristics and clothes, and then retreat to a poky press room where they sketch and colour at high speed.
(19) The residual small rRNAs in [poky] appear to be synthesized via the upstream promoter(s), but are missing 37-44 nucleotides from their 5' ends, indicating either that pre-rRNAs are processed abnormally or that abnormal 5' RNA ends are unstable.
(20) The ribosomal defect shows an extrachromosomal pattern of inheritance in crosses of poky with wild type.
Poly
Definition:
(n.) A whitish woolly plant (Teucrium Polium) of the order Labiatae, found throughout the Mediterranean region. The name, with sundry prefixes, is sometimes given to other related species of the same genus.
Example Sentences:
(1) Only the approximately 2.7 kb mRNA species was visualized in Northern blots of total cellular and poly(A+) RNA isolated from cardiac ventricular muscle.
(2) While both inhibitors caused thermosensitization, they did not affect the time scale for the development of thermotolerance at 42 degrees C or after acute heating at 45 degrees C. The inhibitors of poly(ADP-ribosylation) radiosensitizers and thermosensitizers may be of use in the treatment of cancer using a combined modality of radiation and hyperthermia.
(3) Poly (8NH2G) does not interact with poly(C) in neutral solution because of the high stability of the hemiprotonated G-G self-structure.
(4) Polyadenylic-polyuridylic acid complexes (poly A:U) at the 1-5 mu g level, were mitogenic for spleen cells when given intravenously to normal Balb or cortisone-treated mice.
(5) Analysis of glutamate dehydrogenase (GDH) expression by enzyme assay and immunoblots, as well as Northern and dot blots of poly (A)+ RNA, in the deletion strains indicates that there are two upstream regulatory sequences that control the level of gene expression.
(6) F(ab')2 anti-Ig stimulates the rapid breakdown of inositol phospholipids in B cells, resulting in the prolonged release of inositol (poly)phosphates and diacylglycerol.
(7) Newly synthesized poly(A) associated mRNA with S value of 25 and a stimulation of poly(A) lacking mRNA of 8--9 S and 16--17 S were found in the CA3 nerve cells of trained but not in active controls.
(8) The mutant ribosomes prepared from the transition-phase cells have much lower activity (below 60%) for poly(U)-directed polyphenylalanine synthesis than those in exponentially growing or resting stationary-phase cells.
(9) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(10) Sequences representing the entire TIR are transcribed into poly(A)+ mRNA at both early and late times in the infection.
(11) The poly(dG-dC) helical duplex forms a modified, B-family structure (B*) at very high hydration and a normal B structure at slightly lower hydration.
(12) After size fractionation on a sucrose density gradient, poly(A)+ RNA encoding for the MDCK taurine transporter was found in the fraction whose average size was 4.4 kilobases.
(13) Poly(ortho ester) bioerodible polymers are suitable materials for the topical administration of a wide variety of therapeutic agents; varying the nature and amounts of excipients physically incorporated into the polymer will vary the erosion rates from a few hours to many months.
(14) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(15) The cells, however, were not inhibited by the same concentrations of free poly(Lys) and free drug.
(16) The product AEDANS-S1 can bind to 30S ribosomal subunits lacking S1 as shown by polyacrylamide-agarose gel electrophoresis AEDANS-S1 and AAF-S1 when added back to S1-depleted 30S subunits modulate poly(U)-dependent polyphenylalanine synthesis in the presence of IF3 in a very similar way to unmodified S1.
(17) Both early and late after infection, total nuclear viral transcripts are, on the average, larger than viral transcripts found on polyribosomes; however, nuclear HSV poly(A+) RNA is not measureably larger than the corresponding cytoplasmic viral poly(A+) sequences at either time.
(18) The "random coil" conformational problem is examined by comparison of vibrational CD (VCD) spectra of various polypeptide model systems with that of proline oligomers [(Pro)n] and poly(L-proline).
(19) Infrared linear dichroism investigations indicate that the alternating poly[d(A-T)]-poly[d(A-T)], enzymatically synthesized, adopts a lower humidity a well-expressed A* form in which stability is relatively small,i.e., restricted to limited relative humidity.
(20) The bent DNA has been localized to a 40-55 base pair (bp) segment and contains six (A)3-5 stretches (that is, six poly(A) stretches, three to five nucleotides in length) phased approximately every 10.5 bp.