(n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
(a.) First; original; primary.
(n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
(n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
(n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.
Example Sentences:
(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
(3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
(4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
(5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
(6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
(8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
(9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
(10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
(12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
(19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
(20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
Real
Definition:
(n.) A small Spanish silver coin; also, a denomination of money of account, formerly the unit of the Spanish monetary system.
(a.) Royal; regal; kingly.
(a.) Actually being or existing; not fictitious or imaginary; as, a description of real life.
(a.) True; genuine; not artificial, counterfeit, or factitious; often opposed to ostensible; as, the real reason; real Madeira wine; real ginger.
(a.) Relating to things, not to persons.
(a.) Having an assignable arithmetical or numerical value or meaning; not imaginary.
(a.) Pertaining to things fixed, permanent, or immovable, as to lands and tenements; as, real property, in distinction from personal or movable property.
(n.) A realist.
Example Sentences:
(1) You lot have got real issues to talk about and deal with.
(2) Facebook Twitter Pinterest With a plot based around fake (or real?)
(3) It did the job of triggering growth, but it also fueled real-estate speculation, similar to what was going on in the mid-2000s here.” Slowing economic growth may be another concern.
(4) A good example is Apple TV: Can it possibly generate real money at $100 a puck?
(5) The light intensity profile for any desired cell can be examined in "real time", even during acceleration of the rotor.
(6) It is intended to aid in finding the appropriate PI (proportional-integral) controller settings by means of computer simulation instead of real experiments with the system.
(7) Tap the relevant details into Google, though, and the real names soon appear before your eyes: the boss in question, stern and yet oddly quixotic, is Phyllis Westberg of Harold Ober Associates.
(8) There were soon tales of claimants dying after having had money withdrawn, but the real administrative problem was the explosion of appeals, which very often succeeded because many medical problems were being routinely ignored at the earlier stage.
(9) 75 min: Real Madrid substitution: Angel Di Maria off, Ricky Kaka on.
(10) It is clear that the linking of the naming rights to West Ham United generates real cash value for the LLDC and the taxpayer.
(11) The dual-probe system incorporates a central collimated probe for monitoring activity in the LV surrounded by an annular detector collimated in such a manner as to provide simultaneous real-time monitoring of the LV background activity.
(12) Real ear CVRs, calculated from real ear recordings of nonsense syllables, were obtained from eight hearing-impaired listeners.
(13) Zidane is the 15th manager Real Madrid have had since 2003.
(14) Further studies are required to show whether these differences are real and, if so, whether they have any relevance for the pathogenesis of migraine attacks.
(15) Real Labour would not just meddle with a cosmetic charge on rich London mansions .
(16) Thus, luciferase transcriptional fusions can detect subtle variations in initial rates of gene expression in a real-time, nondestructive assay.
(17) Thus, 10 degrees should be subtracted from the ultrasound values in order to obtain the real AV angles.
(18) It was not certain whether the association was real or what the explanation might be.
(19) "It will mean root-and-branch change for our banks if we are to deliver real change for Britain, if we are to rebuild our economy so it works for working people, and if we are to restore trust in a sector of our economy worth billions of pounds and hundreds of thousands of jobs to our country."
(20) The resulting corner is dealt with easily by Real, who scoot upfield through Di Maria.