(n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
(a.) First; original; primary.
(n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
(n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
(n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.
Example Sentences:
(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
(3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
(4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
(5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
(6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
(8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
(9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
(10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
(12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
(19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
(20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
Say
Definition:
(imp.) Saw.
(n.) Trial by sample; assay; sample; specimen; smack.
(n.) Tried quality; temper; proof.
(n.) Essay; trial; attempt.
(v. t.) To try; to assay.
(n.) A kind of silk or satin.
(n.) A delicate kind of serge, or woolen cloth.
(v. t.) To utter or express in words; to tell; to speak; to declare; as, he said many wise things.
(v. t.) To repeat; to rehearse; to recite; to pronounce; as, to say a lesson.
(v. t.) To announce as a decision or opinion; to state positively; to assert; hence, to form an opinion upon; to be sure about; to be determined in mind as to.
(v. t.) To mention or suggest as an estimate, hypothesis, or approximation; hence, to suppose; -- in the imperative, followed sometimes by the subjunctive; as, he had, say fifty thousand dollars; the fox had run, say ten miles.
(v. i.) To speak; to express an opinion; to make answer; to reply.
(v. t.) A speech; something said; an expression of opinion; a current story; a maxim or proverb.
Example Sentences:
(1) Lebedev says he is aware that he is under investigation.
(2) says Gregg Wallace opening the new series of Celebrity MasterChef (Mon-Fri, 2.15pm, BBC1).
(3) However, some contactless transactions are processed offline so may not appear on a customer’s account until after the block has been applied.” It says payments that had been made offline on the day of cancellation may be applied to accounts and would be refunded when the customer identified them; payments made on days after the cancellation will not be taken from an account.
(4) But when they decided to get married, "finding the clothes became my project," says Melanie.
(5) Mike Ashley told Lee Charnley that maybe he could talk with me last week but I said: ‘Listen, we cannot say too much so I think it’s better if we wait.’ The message Mike Ashley is sending is quite positive, but it was better to talk after we play Tottenham.” Benítez will ask Ashley for written assurances over his transfer budget, control of transfers and other spheres of club autonomy, but can also reassure the owner that the prospect of managing in the second tier holds few fears for him.
(6) Schneiderlin, valued at an improbable £27m, and the currently injured Jay Rodriguez are wanted by their former manager Mauricio Pochettino at Spurs, but the chairman Ralph Krueger has apparently called a halt to any more outgoings, saying: “They are part of the core that we have decided to keep at Southampton.” He added: “Jay Rodriguez and Morgan Schneiderlin are not for sale and they will be a part of our club as we enter the new season.” The new manager Ronald Koeman has begun rebuilding by bringing in Dusan Tadic and Graziano Pellè from the Dutch league and Krueger said: “We will have players coming in, we will make transfers to strengthen the squad.
(7) The idea that 80% of an engineer's time is spent on the day job and 20% pursuing a personal project is a mathematician's solution to innovation, Brin says.
(8) Unlike Milo, he appears to be – to some extent – convinced of the truth of what he’s saying.
(9) It is my desperate hope that we close out of town.” In the book, God publishes his own 'It Getteth Better' video and clarifies his original writings on homosexuality: I remember dictating these lines to Moses; and afterward looking up to find him staring at me in wide-eyed astonishment, and saying, "Thou do knowest that when the Israelites read this, they're going to lose their fucking shit, right?"
(10) Joe, meanwhile, defends her right to say "negro" whenever she wants.
(11) I hope this movement will continue and spread for it has within itself the power to stand up to fascism, be victorious in the face of extremism and say no to oppressive political powers everywhere.” Appearing via videolink from Tehran, and joined by London mayor Sadiq Khan and Palme d’Or winner Mike Leigh, Farhadi said: “We are all citizens of the world and I will endeavour to protect and spread this unity.” The London screening of The Salesman on Sunday evening wasintended to be a show of unity and strength against Trump’s travel ban, which attempted to block arrivals in the US from seven predominantly Muslim countries: Iran, Iraq, Libya, Sudan, Somalia, Syria and Yemen.
(12) 8.47pm: Cameron says he believes Britain's best days lie ahead and that he believes in public service.
(13) Critics say he is unelectable as prime minister and will never be able to implement his plans, but he has nonetheless pulled attention back to an issue that many thought had gone away for good.
(14) A new propaganda video by Islamic State featuring the British photojournalist John Cantlie, in which he says it is the “last film in this series”, has appeared online.
(15) I think he had been saying all season that with three or four games to go he will tell us where we are.
(16) It was with unanimous consent.” He denied that Trump’s tweets had played a part, saying: “No, no, no.
(17) That doesn't necessarily mean she'll like what I'm saying."
(18) This week's unconfirmed claims that Kim's uncle Jang Song Thaek had been ousted from power have refocused attention on the country's domestic affairs; some analysts say Jang was associated with reform .
(19) On the other hand the TUC says people should also be prepared to be out in the sun for several hours and bring sunscreen and if possible a hat.
(20) 'The French see it as an open and shut case,' says a Paris-based diplomat.