(n.) an instrument or device for priming; esp., a cap, tube, or water containing percussion powder or other compound for igniting a charge of gunpowder.
(a.) First; original; primary.
(n.) Originally, a small prayer book for church service, containing the little office of the Virgin Mary; also, a work of elementary religious instruction.
(n.) A small elementary book for teaching children to read; a reading or spelling book for a beginner.
(n.) A kind of type, of which there are two species; one, called long primer, intermediate in size between bourgeois and small pica [see Long primer]; the other, called great primer, larger than pica.
Example Sentences:
(1) The following conclusions emerge: (i) when the 3' or the 3' penultimate base of the oligonucleotide mismatched an allele, no amplification product could be detected; (ii) when the mismatches were 3 and 4 bases from the 3' end of the primer, differential amplification was still observed, but only at certain concentrations of magnesium chloride; (iii) the mismatched allele can be detected in the presence of a 40-fold excess of the matched allele; (iv) primers as short as 13 nucleotides were effective; and (v) the specificity of the amplification could be overwhelmed by greatly increasing the concentration of target DNA.
(2) Elongation of existing RNA primers by the human polymerase-primase was semi-processive; following primer binding the DNA polymerase continuously incorporated 20 to 50 nucleotides, then it dissociated from the template DNA.
(3) This cDNA was obtained because of an identical 10 bp match with the 3' end of one of the GnRH primers.
(4) When PCR products in each of the 12 cats were subjected to a second amplification using the same primer pair (two-step amplification: double PCR), FIV proviral DNA was detected in all of the cats.
(5) In junctions, 3' PSS termini are preserved by fill-in DNA synthesis, although their 5' recessed ends cannot serve as a primer.
(6) Previous studies demonstrated that, when poly(dT).oligo(dA) was used as a template-primer, both proteins were required for poly(dA) synthesis.
(7) 5'-Ends of transcripts of the region were located by S1 nuclease mapping and primer extension.
(8) Nucleotide incorporation kinetics were determined and sequence specific pausing was analyzed by primer-extension.
(9) Primer extension and S1 nuclease protection analysis demonstrate that the hepatic and kidney specific mRNAs are transcriptionally initiated at different sites within the sialyltransferase gene.
(10) alpha 1 and alpha 2 were very similar as DNA polymerases in their sensitivity to several inhibitors and their preference for template-primers, except that alpha 1 had a slightly greater preference for poly (dT) X (rA)10 than alpha 2 did.
(11) Our results indicate that provirus expression occurs by two different mechanisms: one provirus acquired a single base pair mutation in the retrovirus tRNA primer binding site, permitting provirus expression; expression of three proviruses was mediated by 5'-flanking DNA sequences.
(12) Cognate sites in genomes that diverged approximately 100 million years ago can be detected by PCR assays based on primer pairs from unique sequences.
(13) HDV cDNA was then directly amplified with Taq polymerase using three pairs of specific primers.
(14) This reaction involved the synthesis of a short oligoribonucleotide primer which was then extended into a DNA chain.
(15) cDNA was prepared by reverse transcription of peripheral blood mRNA and amplified by the polymerase chain reaction (PCR) using primers corresponding to sequences 400 bp apart on the cDNA, spanning the last three exons (X, Y, Z) of the beta-Sp gene.
(16) B.subtilis phage M2 uses a protein, instead of RNA, as the primer of its DNA replication.
(17) Optimum specific amplification resulted when the primer annealing temperature was 60 degrees C. The gene fragment was amplifiable in 25 different Brucella species and strains.
(18) Primer extension experiments show that in fission yeast transcripts are initiated at the same starting point as in tomato, indicating for the first time that a plant promoter can be correctly recognized in fission yeast.
(19) Genomic clones for the mouse estrogen receptor have been isolated from a cosmid library and used in conjunction with the cDNA clones to study the expression of the receptor in vivo by RNase mapping, primer extension, and Northern blotting.
(20) By using primer 1 (5'-AAAGAATTCATGGAATCCAGGATCTG-3', upstream nucleotides 157 to 2877), primer 2 (5'-AAAGAATTCATGAACGTGAAGGAATCG-3', upstream nucleotides 1846 to 2877), and primer 4(5'-ATAAAGCTTAATCAGACGTTCTCTTCTTC-3', downstream nucleotides 157 to 2877 and 1846 to 2877), the HCMV B gene code region sequence and its glycoprotein 52 kd antigenic domain sequence were amplified from the recombinant plasmid pBH1 DNA containing the HCMV B gene.
Specific
Definition:
(a.) Of or pertaining to a species; characterizing or constituting a species; possessing the peculiar property or properties of a thing which constitute its species, and distinguish it from other things; as, the specific form of an animal or a plant; the specific qualities of a drug; the specific distinction between virtue and vice.
(a.) Specifying; definite, or making definite; limited; precise; discriminating; as, a specific statement.
(a.) Exerting a peculiar influence over any part of the body; preventing or curing disease by a peculiar adaption, and not on general principles; as, quinine is a specific medicine in cases of malaria.
(n.) A specific remedy. See Specific, a., 3.
(a.) Anything having peculiar adaption to the purpose to which it is applied.
Example Sentences:
(1) Comparison of the S100 alpha-binding protein profiles in fast- and slow-twitch fibers of various species revealed few, if any, species- or fiber type-specific S100 binding proteins.
(2) Unfortunately, due to confidentiality clauses that have been imposed on us by the Department of Immigration and Border Protection, we are unable to provide our full names and … titles … However, we believe the evidence that will be submitted will validate the statements that we are making in this submission.” The submission detailed specific allegations – including names and dates – of sexual abuse of child detainees, violence and bullying of children, suicide attempts by children and medical neglect.
(3) Anti-Leu 7 could not be considered as a specific marker for oligodendroglioma.
(4) Five probes of high specificity to individual chromosomes (chromosomes 3, 11, 17, 18 and X) were hybridized in situ to metaphase chromosomes of different individuals.
(5) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
(6) The role of Ca2+ in cell agglutination may be either to activate the cell-surface dextran receptor or to form specific intercellular Ca2+ bridges.
(7) The Cole-Moore effect, which was found here only under a specific set of conditions, thus may be a special case rather than the general property of the membrane.
(8) Although solely nociresponsive neurons are clearly likely to fill a role in the processing and signalling of pain in the conscious central nervous system, the way in which such useful specificity could be conveyed by multireceptive neurons is difficult to appreciate.
(9) On the other hand, after exposure to BrdUrd, neuron specific enolase decreased in NB-1 and SK-N-DZ and increased in GOTO.
(10) Most of the radioactivity in spleen cells from these rats were associated with antigen-reactive cells which formed rosettes specifically with HO erythrocytes.
(11) The origins of aging of higher forms of life, particularly humans, is presented as the consequence of an evolved balance between 4 specific kinds of dysfunction-producing events and 4 kinds of evolved counteracting effects in long-lived forms.
(12) The specific activities of extracts from cells grown under phototrophic and aerobic conditions were similar and not affected by the concentration of iron in the growth media.
(13) Proliferation assays using F3 showed that 15 (14 CD4+ and 1 CD8+) of the 18 isolated clones were specific for T. gondii.
(14) We have developed a new procedure for the rapid preparation of undegraded total RNA from cultured cells for specific quantitation by dot blotting analysis.
(15) Further studies are required to elucidate specific roles of the steroid-induced proteins in the effects of glucocorticoids on HTM and HS cells.
(16) In addition, the fact that microheterogeneity may occur without limit in the mannans of the strains suggests that antibodies with unlimited diverse specificities are produced directed against these antigenic varieties as well.
(17) Four other independent LCMV-GP2(275-289) specific H-2Db-restricted CTL clones also expressed V alpha 4 and V beta 10 gene elements.
(18) Training in social skills specific to fostering intimacy is suggested as a therapeutic step, and modifications to the social support measure for future use discussed.
(19) When compared with self-reported exposures, the sensitivity of both job-exposure matrices was low (on average, below 0.51), while the specificity was generally high (on average, above 0.90).
(20) The specific limited trypsinolysis of bacteriophage T7 RNA polymerase (T7RP) was performed in the presence of various components of the polymerase reaction and some GTP-analogs--irreversible inhibitors of the enzyme.