What's the difference between proteolytic and rennin?
Proteolytic
Definition:
(a.) Converting proteid or albuminous matter into soluble and diffusible products, as peptones.
Example Sentences:
(1) Digestion is initiated in the gastric region by secretion of acid and pepsin; however, diversity of digestive enzymes is highest in the post-gastric alimentary canal with the greatest proteolytic activity in the spiral valve.
(2) The resistance of GSA 65 to proteolytic degradation, together with previous immunofluorescence data that indicate the antigen is an integral part of the G. lamblia cyst wall, suggests that this molecule may play a role in maintaining the integrity of the cyst in vivo.
(3) 5% of the degradation resulted from enzymatic activity in the culture medium, presumably due to secretion of proteolytic enzymes by the cells.
(4) It is the action of this protease that releases the enzyme from the membrane, as shown by the observations that protease inhibitors decreased the amount of solubilization of the enzyme, and the enzyme remaining in the membrane after heating showed much less proteolytic cleavage than that which was released.
(5) Three distinct antigenic regions of bovine somatotropin (bST) were identified on the basis of the ability of a set of monoclonal antibodies to bind to proteolytic fragments and deletion variants of recombinant bST (rbST) in Western blot analyses.
(6) Radio-immunoprecipitation and partial proteolytic digest mapping showed that the monoclonal antibodies each recognized a unique epitope.
(7) The fact that proteolytic activity could be detected within 2 days at 7 degrees C is significant, since bulk cooled milk is normally held for 3 to 4 days at temperatures between 4 and 7 degrees C at farms or factories prior to processing.
(8) These results would suggest that N-terminal acetylation and C-terminal proteolytic cleavage are important post-translational modifications of the forms of Amia beta-endorphin.
(9) For some proteins, properly folded protein may be obtained by secretion from E. coli; however, secretion does not ensure correct folding and protection from proteolytic degradation.
(10) Proteolytic activity of cell extracts from revertants of Shigella flexneri L-forms as well as biochemical properties of these strains and their sensitivity to antibiotics were studied.
(11) A pathogenetic mechanism is postulated to explain the subacute evolution of fluid collection with diffusion of proteolytic enzymes between the splenic capsule and parenchyma.
(12) The high proteolytic activity of BCC demonstrated in this study may be an important factor in the proliferative, invasive and destructive behaviour of this tumour.
(13) Human leukocyte elastase can be proteolytically inactivated by bovine pancreatic trypsin.
(14) With ribosomes the pH optimum of proteolytic breakdown is at about 7.
(15) The results indicate that ruthenium red appears to protect against hepatocellular injury by inhibiting degradative proteolytic activity.
(16) The antigens were solubilized by treating the tissue samples with the proteolytic enzymes collagenase, trypsin and pepsin.
(17) The crystal structure of proteolytically modified human ACT has been solved at 2.7-A resolution (Baumann et al., 1991).
(18) The results demonstrate that the D1 protein is degraded by a serine-type of proteolytic activity that is an integral part of photosystem II.
(19) Ca2+ activated proteolytic activity also increases with advance in age.
(20) These data indicate that maturation of the F1 beta-subunit precursor is dependent on a protein sequence located distal to the proteolytic maturation site which is distinct from the mitochondrial targeting sequence.
Rennin
Definition:
(n.) A milk-clotting enzyme obtained from the true stomach (abomasum) of a suckling calf. Mol. wt. about 31,000. Also called chymosin, rennase, and abomasal enzyme.
Example Sentences:
(1) Whey obtained by acid precipitation or by the application of rennin was devoid of bactericidal activity but was capable of slowing down proliferation of E coli.
(2) Mucor rennin was efficiently excreted from the yeast host as a heavily glycosylated form.
(3) One-day-old rats have a protease with a pH optimum of 3.8 to 4.2 similar to that of calf rennin.
(4) Another expression system for production of Mucor rennin in Saccharomyces cerevisiae was also established.
(5) The greatest differences between experimental and control cheeses (produced with rennin) in the contents of the above-mentioned constituents were observed within the first 30 days.
(6) The lactogenic response of mouse mammary gland explants to human placental lactogen (hPL) and ovine pituitary prolactin (oPRL) was examined on days 10 to 18 of pregnancy by measuring 3H-amino acid incorporation into calcium-rennin precipitable casein.
(7) Activation of the four separate components of prochymosin (prorennin) at pH 5.0 demonstrated that each zymogen was the precursor to an electrophoretically distinct chymosin (rennin).
(8) Polyacrylamide-gel electrophoresis confirmed the chromatographic results, but crystalline rennin was shown to consist of four bands.
(9) This was done using the yeast GAL7 promoter and the prepeptide sequence of a fungal aspartic proteinase, Mucor pusillus rennin (MPR).
(10) The curves were characteristic of a limited, specific attack by rennin on these proteins.
(11) The stomach of newborn pig contains a proteinase that is immunologically closely related to calf chymosin (rennin) (EC 3.4.23.4.).
(12) It is concluded that a I:I mixture of porcine pepsin and rennin may be used for the production of small medium hard cheeses without impairing their quality of nutritive value.
(13) Both milk-clotting proteases have their optimum activity at pH 5.2 and 45 degrees C. The microbiological rennin has a second maximum activity at pH 3.5 and 55 degrees C. Temperatures above 55 degrees C cause a rapid decrease of activity.
(14) The prepro-peptide of fungal aspartic proteinase, Mucor pusillus rennin, is useful as a secretion leader for efficient secretion of human growth hormone (HGH) from Saccharomyces cerevisiae.
(15) Comparative studies have been made on the effects of diazoacetyl-DL-norleucine methyl ester (DAN), 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) and pepstatin on acid proteases, including those from Acrocylindrium sp., Aspergillus niger, Aspergillus saitoi, Mucor pusillus, Paecilomyces varioti, Rhizopus chinensis, and Trametes sanguinea, and also porcine pepsin [EC 3.4.23.1] and calf rennin [EC 3.4.23.4] for comparative purposes.
(16) Simple, reliable procedures for the assay of pepsin and rennin-like enzyme activities are described as a means of identifying gastric fluid-containing samples in forensic science laboratories.
(17) Aspergillus flavus produced extracellularly an active rennin-like enzyme when grown aerobically in whey media.
(18) When anti-sera to bovine pepsinogen and chymosin (rennin) was used, immunoreactive tumor cells were found in 12 of 23 gastric adenocarcinomas irrespective of the tumor subtype, degree of differentiation, or the presence or absence of intestinal metaplasia in the adjacent gastric mucosa.
(19) Calf Chymosin and a fungal protease from Mucor pusillus (Mucor rennin) are members of the aspartic proteinases used as milk-coagulants in cheese industry.
(20) The role of individual amino acid residues in the 98-102 and 111-112 regions of bovine kappa-casein in its interaction with the milk-clotting enzyme chymosin (rennin) was investigated.