(1) 25% of the incorporated radioactivity in protoplast lysates and approx.
(2) We have isolated an auxin-regulated cDNA, parB, from the early stage of cultured tobacco mesophyll protoplasts.
(3) Several subcellular fractions were derived from OK-432 and only the cytoplasmic and protoplast membrane fractions showed cytotoxic activity against the OK-432-sensitive tumor cell lines, although the cytotoxicity obtained was greatly less than the whole microorganism OK-432.
(4) The pH activity profile, cofactor requirements, and kinetic parameters of the endogenously activated chitin synthase were identical to those of the trypsin-activated enzyme in protoplast membranes.
(5) Osmotic gradient across the membrane of nonsonicated liposomes and rose petal protoplasts are shown to induce swelling.
(6) Unlike malate oxidation by osmotically shocked protoplasts, endogenous protoplast repiration was resistant to ferricianide 5.10(-4) M).
(7) The inhibition was also observed when cells were incubated with mercaptoethanol or when mercaptoethanol and DTT were used to prepare protoplasts.
(8) Regeneration of cells from protoplasts was monitored throughout the growth cycle and was most efficient when cells of either S. fradiae or S. griseofuscus were taken from the transition phase between the exponential and stationary growth phases.
(9) (2) Protoplast fusion analysis indicates that rosamicin-nonproducing characteristics of MR 217-AF2 and MR 217-AF3 strains induced by the acriflavine treatment is due to chromosomal mutation or rearrangement but not to loss of a plasmid.
(10) When electroporated into maize protoplasts from a suspension cell line not synthesizing anthocyanins, reporter genes with Bz2, Bz1, and A1 promoters are expressed only when both R and C1 expression plasmids are co-electroporated.
(11) In the presence of a suitable carbon source, whole cells and protoplasts of Saccharomyces cerevisiae synthesized glycerol as a compatible organic solute in response to increased external osmotic pressure.
(12) Tobacco protoplasts were transformed with an expression construct containing a translational fusion between mature alpha-amylase from Bacillus licheniformis and the signal peptide of the tobacco PR-S protein.
(13) The number of binding sites on bacilli and protoplasts is determined for each phage.
(14) In both the experiments there were detected cells in their majority with thinner walls, L-form-like structures, protoplasts and single conglomerates of the cells with thicker walls and anomalous division and the cells at the moment of lysis.
(15) Rate determinations were made with both, intact cells and with preparations containing secreted enzymes from protoplasts.
(16) The effects of Mg2+ and Ca2+ ions on the efficiency of the plasmid transformation of lysozyme-treated Streptococcus lactis protoplasts were compared.
(17) Tnt1 has been isolated after its transposition into the nitrate reductase (NR) structural gene of tobacco, and transposition events have been detected through in vitro selection of spontaneous NR-deficient (NR-) mutant lines in cell cultures derived from tobacco mesophyll protoplasts.
(18) The effect of treatment of the protoplasts and cell membranes of C. albicans with polyenic entibiotics on formation of the ribosomal-membrane complex was studied in vitro.
(19) On the other hand, R medium without MgCl2 but with penicillin G supported development of L-form type colonies at high rate (13-15%) from the inoculated protoplasts.
(20) In comparison, in protoplasts a region upstream of the AC2 open reading frame was shown to have moderate promoter activity.
Prototypical
Definition:
Example Sentences:
(1) Variability (CV = 0.7%) in body volume of a 45-year-old reference man measured by SH method was very similar to variation (CV = 0.6%) in mass volume of the 60-1 prototype.
(2) Infants were habituated to models posing either prototypically positive displays (e.g., happy expressions) or positive expression blends (e.g., mock surprise).
(3) The nucleotide and amino acid sequences of the M RNA of Bunyamwera virus (prototype of the serogroup) and snowshow hare and La Crosse viruses (California serogroup) (Lees et al., 1986; Eshita and Bishop, 1984; Grady et al., 1987) were compared to those of Germiston virus.
(4) There are several known enzymes recognizing the same sequence, although the prototype NarI and isoschizomers NdaI and NunII cleave the substrate to produce 5'-protruding ends, whereas cleavage with isoschizomer BbeI results in 3'-protruding ends.
(5) The two new genome types differ from the prototype Ad7c virus in having two (Ad7c1) or one (Ad7c2) extra cleavage sites for the restriction endonuclease EcoRI.
(6) This entity belongs to the group of endocrine duodenal ulcer diatheses of which the Zollinger-Ellison Syndrome (ZES) is the prototype.
(7) We compared viral DNA sequences in prototype EBV strains with data presented in the Virology paper.
(8) Results of a four year clinical study of the prototype involving 1743 patients are reported.
(9) This assay is a prototype study of human response to therapy in relation to tumor antigen and host response, as measured by a nonspecific tumor associated antigen.
(10) From these 26 responses the authors have structured a prototypic call schedule and presented its application.
(11) Two different prototypes of columella materials made from aluminum oxide ceramics were newly designed by the author for ossicular reconstruction.
(12) The sequence of molecular clones of the genome of MVM(i), a lymphotropic variant of minute virus of mice, was determined and compared with that of MVM(p), the fibrotropic prototype strain.
(13) The prototype system, which can operate from 2 Hz to 10 kHz, covers most of the frequency range of interest in the encephalographic research.
(14) The optical and thermic properties of the catheter prototypes were determined by physical methods.
(15) A prototype system, termed an acoustic plethysmograph, was built and used to measure the volume of newborn miniature pigs.
(16) A Spinal Pedicle Finder (S.P.F) has been designed for transpedicular screws and a prototype has been completed.
(17) Serogrouping of Bacteroides nodosus is based on antigenic differences in fimbriae of the different New Zealand prototype strains.
(18) We suggest that GHB may serve as the prototype for a new class of hypnotic compounds derived from natural sources and capable of activating the neurological mechanisms of normal human sleep.
(19) Facebook Twitter Pinterest Farage ’flattered’ by Trump’s call for him to be US ambassador In another shot at Obama, referring to remarks by the US president before the Brexit vote about the possible trade consequences of Britain leaving Europe, Farage said: “No longer do we have a president who says that we’re at the back of the line.” Everything you need to know about Trump and the Indiana Carrier factory Read more He also said Hillary Clinton, Trump’s opponent, had “wanted the European Union to be a prototype for a bigger model across the whole world”.
(20) Strains of AV10, 24, and 27 were identical or only slightly different from the respective prototypes.