(1) A Puf- strain of Rhodobacter sphaeroides (PUFB1) was constructed by deleting a portion of the proximal region of the puf operon and inserting a kanamycin resistance gene cartridge.
(2) Chromosome banding patterns are very similar between tissues, but puffing patterns show considerable differences.
(3) The adjacent region, 23B2, is slightly puffed and displays typical RNP particles, some of which may be observed close to band 2-23B1,2.
(4) At the same time the RNA quantity increases by a factor of 2. thermal denaturation profiles of formaldehyde fixed chromosomes show that the Tm of this region in puffed and non puffed state differ by 10 degrees C. Moreover these profiles suggest that a large fraction of histone-bound DNA is destabilized during puffing.
(5) He huffed and puffed, gazed at the heavens at times, and at one point he accused the country’s foremost human rights officer of verballing him.
(6) Habitual smokers of perforation-ventilated cigarettes and of channel-ventilated cigarettes (18 male and 18 female subjects each; nicotine yield 0.1-0.3 mg, 0.2 mg, respectively) were compared with respect to different smoke exposure indicators and puffing behavior.
(7) The boosts for perforation-ventilated cigarettes remained unchanged and were reached with only moderately intensified puffing behavior.
(8) Where there were pictures of powerful women, the images tended to be subversive: the same photograph of a grimacing Theresa May was used to illustrate three different stories about the home secretary, and two of the three pictures of the German chancellor showed Angela Merkel puffing out her cheeks, looking mildly absurd.
(9) People don’t have sex within only one borough – an example of why balkanisation is more expensive than collectivism The immediate anxiety was that elected officials are often not public health experts: you might get a very enlightened council, who understood the needs of the disenfranchised and prioritised them; or you might get a bunch of puffed-up moralists who spent their syphilis budget on a new aqua aerobics provision for the overweight.
(10) Southern blot analysis demonstrated that in PUFB1, the defective copy of the puf operon had replaced, through homologous recombination, the normal chromosomal copy.
(11) Heat a little oil in a pan then cook the dumplings until crisp and puffed, then roll in the cinnamon sugar.
(12) Fluorescence polarization measurements of the lipopholic probe, 1, 6-diphenyl-1,3,5-hexatriene (DPH), in the purified lymphocyte plasmalemma from mice fed a diet high in PUF indicated an increase in mobility.
(13) The pufBA messages, which end in a large intercistronic stem-loop structure, are long-lived processing products of the puf operon transcripts.
(14) The best subset of predictors, especially with respect to puffing parameters, was found to vary considerably across smoking conditions and dependent variables.
(15) The influence of temperature (17 and 31 degrees) on the maternal effect of mutation Puffed (Pu) in Drosophila hybrids has been studied.
(16) Last month I was given unrestricted access to the enormous archive the PCGG has assembled in its years of global detective work: the president’s handwritten diary, frequently puffed with self-regard; the notepaper headed “From the office of the president”, with scribbled sums endlessly totting up his cash; minutes of company meetings with his comments scrawled in the margins; contracts; “side agreements”; records of multiple bank accounts; hundreds of share certificates; private investigators’ reports; and tens of thousands of pages of court judgments.
(17) The polytene chromosome puffing patterns of Drosophila guanche were established and compared with those of Drosophila subobscura.
(18) David Cameron was “pumped up” ; Russell Brand and Ed Miliband exchanged “aint’s” and “innits” and puffed out their chests.
(19) The individual response to smoke might be assessed by an analysis of puffing on a single cigarette.
(20) Neither between-subject consistency nor within-subject reproducibility was improved by this paced puffing procedure, despite apparent topographical control.
Puffer
Definition:
(n.) One who puffs; one who praises with noisy or extravagant commendation.
(n.) One who is employed by the owner or seller of goods sold at suction to bid up the price; a by-bidder.
(n.) Any plectognath fish which inflates its body, as the species of Tetrodon and Diodon; -- called also blower, puff-fish, swellfish, and globefish.
(n.) The common, or harbor, porpoise.
(n.) A kier.
Example Sentences:
(1) For real.” A resident in a green puffer jacket emerged from the shelter with her 10-year-old son.
(2) Puffers were collected one week after the occurrence of the food poisoning and their content of toxin was determined.
(3) Also featured are the puffer fish, dung beetle, veiled chameleon and moon jellyfish.
(4) The potent neurotoxin tetrodotoxin, which has previously been found in puffer fish of the order Tetraordontiformes, a goby (Gobius criniger), and the California newt (Taricha torosa), has now been identified in the skins of frogs of the genus Atelopus from Costa Rica.
(5) Liver protein synthesis, assayed by a rapid pulse injection technique, showed a moderate temperature dependency (Q10 = 2-3) in the 15-30 degree C range for all species except puffers (Q10 = 10-20).
(6) "Whole-cell" patch recordings using nystatin permeabilization were made from single human platelets during application of agonists from a "puffer" pipette.
(7) The temperature dependency of protein synthesis was studied in vivo in five species of Pacific fish collected in the Galapagos and Perlas Islands: batfish (Ogcocephalus darwini), groupers (Epinephelus labriformis), catfish (Netuma platypogan), puffers (Arothron hispidus) and triggerfish (Sufflamen verres).
(8) "Pink puffers" with breathlessness, hyperinflation, mild hypoxemia, and a low PCO2 are contrasted with "blue bloaters" with hypoxemia, secondary polycythemia, CO2 retention, and pulmonary hypertension and cor pulmonale.
(9) Suppression of Iout was also observed during puffer applications of either of two protein kinase C activators, phorbol 12-myristate 13-acetate (10 nM-1 microM) and 1-oleoyl-2-acetylglycerol (60 microM).
(10) Responsiveness of 143 preoptic neurons to changes in hypothalamic temperature and to non-thermal emotional stimuli were investigated while rewarding (foods) and aversive objects (hypertonic saline, a toy snake, an air puffer) were given.
(11) Three conditions that may occur after consumption of seafood--puffer fish poisoning, ciguatera, and paralytic shellfish poisoning--are caused by a group of poisons that block voltage-gated sodium channels in myelinated and non-myelinated nerves.
(12) The Na and K concentration in single supramedullary neurons of the puffer fish (Spheroides maculatus) was measured using a dual channel integrating ultramicroflame photometer.
(13) If salbutamol was one breakthrough, the later introduction of steroid inhalers (which are brown, as opposed to the blue reliever puffers), which prevent symptoms rather than relieve them, was even more significant.
(14) He arrived without entourage or announcement, unzipped his puffer jacket, shook Skip’s hand, and – after greeting everyone in the room – took a seat on the side of the room and asked to get to work.
(15) Unique exocrine glands or gland-like structures were found in the skin of several species of puffer fishes of the genus Takifugu.
(16) Bicuculline methiodide reversibly blocked THIP- and muscimol-induced suppressions of tactile- (air puffer)-induced S1 responses but spared those produced by (-)-baclofen.
(17) The aim of this study was to assess the effects of diamorphine on breathlessness and exercise tolerance in patients with severe chronic airflow obstruction and normal arterial carbon dioxide tension (PCO2) levels ("pink puffer" syndrome).
(18) Chubby Puffer syndrome produces symptoms such as sleep apnea, cor pulmonale and upper airway obstruction due to adenotonsillar enlargement.
(19) As the classic "blue bloater" with attenuated respiratory drive is described as being less dyspneic than his "pink puffer" counterpart, we wondered whether the variability in dyspnea and exercise tolerance in a group of patients with COPD with relatively similar degrees of air-flow obstruction might be partly explained by the variability in resting respiratory drives (unstimulated P0.1 and hypoxic and hypercapnic P0.1 responses).
(20) Tetrodotoxin (puffer fish toxin) or saxitoxin (paralytic shellfish poison), both of which block the sodium channel of excitable membranes, antagonize this effect, enabling cell growth to continue.