(1) Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes.
(2) Mung bean nuclease protection analysis shows that the puh transcripts have different 5' termini within open reading frame G115 and a unique rho-independent termination signal within open reading frame I2372.
(3) The occurrence of overlapping transcripts for the puf and puh operons was further shown to influence development of the photochemical apparatus during conditions of environmental shifts in oxygen tension.
(4) These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely.
(5) Gene puh itself is transcribed as two mRNAs of 1118 and 1032 nucleotides.
(6) Open reading frame G115, upstream of structural gene puh, is transcribed as a 2.25-kilobase mRNA.
(7) The lifetimes of the puh messages, as determined by an oxygen blockade of transcription, were 10 and 12 min for the large and small puh mRNAs, respectively.
(8) In addition, the location and relative distance observed between the puf and puh operons which encode for light harvesting and reaction-centre structural genes are also conserved between these species.
(9) The mRNA transcripts of Rhodospirillum rubrum gene puh, coding for the H subunit of the photoreaction center, and of genes flanking puh were analyzed by blot hybridization.
(10) The Rhodospirillum rubrum structural gene puh, coding for the photoreaction center H polypeptide, and three other putative genes that surround puh were cloned and sequenced.
(11) In this study we demonstrate that the puh operon, which is located 39 kb away from the puf operon, also contains overlapping transcripts.
(12) Thus, in the mutants, RNA polymerase bound at the strong (mutant) PUH site effectively repress the PC promoter.
(13) Wave forms were selected from BAEPs performed in the last four years at the University of Pittsburgh Presbyterian University Hospital (PUH).
(14) His scattergun fluency is still punctuated by almost comically exaggerated displays of admiration or indignation: " Puh-leeze! "
(15) Although the mutations lie far outside PC, they cause PC to be inactive, apparently because binding of RNA polymerase to the PUH promoter blocks the overlapping PC site.
(16) Length of implantation has varied from 1 to 48 days in the PUH series.
(17) From a genomic cosmid library of R. viridis constructed in the vector pHC79, clones that coded for subunits H (puh operon), L, M and cytochrome c (puf operon) of the photosynthetic reaction center were isolated and characterized.
(18) During this shift in growth conditions, the puf and puh mRNAs, coding for structural polypeptides of the photosynthetic apparatus, underwent a six- to eightfold induction, while the amount of mRNA from the fbc locus remained constant.
Pun
Definition:
(v. t.) To pound.
(n.) A play on words which have the same sound but different meanings; an expression in which two different applications of a word present an odd or ludicrous idea; a kind of quibble or equivocation.
(v. i.) To make puns, or a pun; to use a word in a double sense, especially when the contrast of ideas is ludicrous; to play upon words; to quibble.
(v. t.) To persuade or affect by a pun.
Example Sentences:
(1) Go Kings go!” The pun-filled press release issued by De Blasio also helpfully included the lyrics to Sinatra’s and Newman’s classic tunes, in case anyone had forgotten.
(2) Bad pun aside, investors are concerned that the company's high growth-rates are tapering.
(3) January 12, 2016 Shorten hastily responded to that debate on Twitter with a pun-laden non-answer, saying: “Cos you asked … my favourite lettuce is one that doesn’t have a 15 per cent GST on it.” Bill Shorten (@billshortenmp) Cos you asked @workmanalice - my favourite lettuce is one that doesn't have a 15 per cent GST on it.
(4) The following day, politicians and eurocrats began scrambling to hammer out a larger rescue package for Greece: 28 April 2010 Photograph: Guardian That was the time when puns about Acropolis Now, and ‘making a drachma out of a crisis’ were in vogue: Greek debt crisis, 28 April 2010 Photograph: Guardian But there wasn’t much time for jokes.
(5) Hemingway’s daughter, Corey, is in a marquee at the back of the site, painting a teddy bear onto some MDF, in the pursuit of a Teddy Boy pun that either doesn’t work, or I don’t get, but it looks great.
(6) The outcome of 53 patients operated on either for posttraumatic ulnar neuropathy (PUN) or non-traumatic cubital tunnel syndrome (CTS) was reviewed after 3 years follow-up.
(7) In a report called "Un-Finnished Business" (you can always rely on the rating agencies for a bad pun), the analysts write: We think the risks to growth in 2012 and 2013 are rising.
(8) In all experiments, supplementing TRP-deficient diets with D- and L-TRP significantly increased feed intake, rate and efficiency of gain and decreased plasma urea N (PUN).
(9) Systemic LH, FSH, glucagon, cortisol, PUN, NEFA, estradiol, and testosterone were not affected by insulin or level of feeding.
(10) The first of April is normally a day of frothy fun, where newspapers and brands compete to produce the best jokes and the worst puns to fool their readers.
(11) The unfortunate design hasn’t gone unnoticed and attracted puns galore when it was posted on Reddit over the weekend .
(12) Photograph: David Levene for the Guardian The signs of wealth are everywhere, from the luxury gated village of the Pun Hlaing Golf Estate to the towers around swimming pools of Star City , both projects of the Pun empire.
(13) Emad Hajjaj, a popular Jordanian cartoonist, drew an elderly Palestinian woman by her sagging UN tent saying – in an untranslatable pun on the words “Charlie” and the colloquial Arabic “I have been” – that she had lived as a refugee for the 67 years since the creation of Israel in 1948.
(14) During the war years, his snappy, escapist films brought joy to audiences on the home front, while he was the only Hope (puns on his surname have always been de rigueur ) for thousands of troops overseas whom he entertained on his various tours from 1941.
(15) Second, the yuck factor: isn't it just beyond tasteful (no pun intended) for a woman to put her nipple into another woman's baby's mouth?
(16) The report tackles a number of issues which, excuse the pun, have been ‘bubbling’ up over the last year.
(17) Sorry and all that, but the pun was too good to use: Genre?
(18) Was The Wine Show supposed to be a deliberately awful pun on The One Show?
(19) The comedian Rob Auton, 30, has seen off competition from acclaimed pun-slingers including Tim Vine and Gary Delaney to pick up TV channel Dave's annual award for one-liners at the fringe.
(20) An experiment was conducted with 36 crossbred finishing pigs (18 male castrates and 18 females) to evaluate the effect of bromocriptine implants on growth, feed intake, feed efficiency, plasma urea nitrogen (PUN) and carcass characteristics.