(1) Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes.
(2) Mung bean nuclease protection analysis shows that the puh transcripts have different 5' termini within open reading frame G115 and a unique rho-independent termination signal within open reading frame I2372.
(3) The occurrence of overlapping transcripts for the puf and puh operons was further shown to influence development of the photochemical apparatus during conditions of environmental shifts in oxygen tension.
(4) These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely.
(5) Gene puh itself is transcribed as two mRNAs of 1118 and 1032 nucleotides.
(6) Open reading frame G115, upstream of structural gene puh, is transcribed as a 2.25-kilobase mRNA.
(7) The lifetimes of the puh messages, as determined by an oxygen blockade of transcription, were 10 and 12 min for the large and small puh mRNAs, respectively.
(8) In addition, the location and relative distance observed between the puf and puh operons which encode for light harvesting and reaction-centre structural genes are also conserved between these species.
(9) The mRNA transcripts of Rhodospirillum rubrum gene puh, coding for the H subunit of the photoreaction center, and of genes flanking puh were analyzed by blot hybridization.
(10) The Rhodospirillum rubrum structural gene puh, coding for the photoreaction center H polypeptide, and three other putative genes that surround puh were cloned and sequenced.
(11) In this study we demonstrate that the puh operon, which is located 39 kb away from the puf operon, also contains overlapping transcripts.
(12) Thus, in the mutants, RNA polymerase bound at the strong (mutant) PUH site effectively repress the PC promoter.
(13) Wave forms were selected from BAEPs performed in the last four years at the University of Pittsburgh Presbyterian University Hospital (PUH).
(14) His scattergun fluency is still punctuated by almost comically exaggerated displays of admiration or indignation: " Puh-leeze! "
(15) Although the mutations lie far outside PC, they cause PC to be inactive, apparently because binding of RNA polymerase to the PUH promoter blocks the overlapping PC site.
(16) Length of implantation has varied from 1 to 48 days in the PUH series.
(17) From a genomic cosmid library of R. viridis constructed in the vector pHC79, clones that coded for subunits H (puh operon), L, M and cytochrome c (puf operon) of the photosynthetic reaction center were isolated and characterized.
(18) During this shift in growth conditions, the puf and puh mRNAs, coding for structural polypeptides of the photosynthetic apparatus, underwent a six- to eightfold induction, while the amount of mRNA from the fbc locus remained constant.
Pup
Definition:
(n.) A young dog; a puppy.
(n.) a young seal.
(v. i.) To bring forth whelps or young, as the female of the canine species.
Example Sentences:
(1) Blood was collected from pups and dams to determine its caffeine concentration.
(2) The PUP founder made the comments at a voters’ forum and press conference during an open day held at his Palmer Coolum Resort, where he invited the electorate to see his giant robotic dinosaur park, memorabilia including his car collection and a concert by Dean Vegas, an Elvis impersonator.
(3) These episodes continued for the duration of the suckling test and were enhanced when a second pup was placed on an adjacent nipple.
(4) A considerably greater increase in the peak plasma OT concentration resulted when hungry foster litters of 6 pups were suckled after the mothers' own 6 pups had been suckled.
(5) At 24 days of age, the pups of HP, M and M-F diet groups, only gained 48%, 30% and 18% respectively, in their body weight, whereas the body-length parameters (LNC and LNRC) showed a reduction of 20%, 35%, and 45%, respectively for the same diet groups.
(6) The results also suggest that both alkali metals most probably have been delivered to the suckling pups and some of their toxic effect was retarded.
(7) The PUP leader told the ABC his announcement would have international significance.
(8) The effects of quinpirole and sulpiride on dopamine (DA), dihydroxyphenylacetic acid (DOPAC) and accumulation of dihydroxyphenylalanine (DOPA) after inhibition of DOPA decarboxylase were determined for the striatum and tegmentum of 11- and 17-day-old rat pups.
(9) These observations indicated a novel mechanism that in the absence of light-dark schedule, mothers taught the circadian rhythm to the pups as they raised them.
(10) Sympathetic nervous system function was blocked in developing male SHR by treating pups from days 0 to 14 with: (1) guanethidine, (2) combined alpha- and beta-receptor antagonists (prazosin and timolol), or (3) vehicle (5% sucrose).
(11) Saline-injected controls started gathering the pups immediately and usually showed all elements of maternal behaviour within 10 min.
(12) Rooting latency showed a significant additive maternal strain effect but little systematic effect of pup genotype.
(13) Only six (43 per cent) of the stores routinely informed clients of the need to continue deworming procedures once the pup left the store.
(14) Reinstitution of suckling after removal of pups causes an immediate rise in PRL and GH.
(15) Pups were weaned either to the diet of their dam or to the diet fed to dams in the other treatment group in a crossover design.
(16) Progressive paraparesis developed in four male English Springer Spaniel pups from a litter of five during the first 10 weeks of life.
(17) 5beta-Dihydrotestosterone was adminstered to mothers for 4 days from Day 12 to Day 15 of pregnancy (prenatal treatment) and to pups for 5 days of postnatal life (neonatal treatment) at daily doses of 1 mg and 200 mug, respectively.
(18) The weight and size of the eyeballs were almost the same as those in normal NC pups.
(19) Their pups continued to consume the caffeine diet until 50 days.
(20) The pups were divided into caffeine and noncaffeine groups.