(1) Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes.
(2) Mung bean nuclease protection analysis shows that the puh transcripts have different 5' termini within open reading frame G115 and a unique rho-independent termination signal within open reading frame I2372.
(3) The occurrence of overlapping transcripts for the puf and puh operons was further shown to influence development of the photochemical apparatus during conditions of environmental shifts in oxygen tension.
(4) These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely.
(5) Gene puh itself is transcribed as two mRNAs of 1118 and 1032 nucleotides.
(6) Open reading frame G115, upstream of structural gene puh, is transcribed as a 2.25-kilobase mRNA.
(7) The lifetimes of the puh messages, as determined by an oxygen blockade of transcription, were 10 and 12 min for the large and small puh mRNAs, respectively.
(8) In addition, the location and relative distance observed between the puf and puh operons which encode for light harvesting and reaction-centre structural genes are also conserved between these species.
(9) The mRNA transcripts of Rhodospirillum rubrum gene puh, coding for the H subunit of the photoreaction center, and of genes flanking puh were analyzed by blot hybridization.
(10) The Rhodospirillum rubrum structural gene puh, coding for the photoreaction center H polypeptide, and three other putative genes that surround puh were cloned and sequenced.
(11) In this study we demonstrate that the puh operon, which is located 39 kb away from the puf operon, also contains overlapping transcripts.
(12) Thus, in the mutants, RNA polymerase bound at the strong (mutant) PUH site effectively repress the PC promoter.
(13) Wave forms were selected from BAEPs performed in the last four years at the University of Pittsburgh Presbyterian University Hospital (PUH).
(14) His scattergun fluency is still punctuated by almost comically exaggerated displays of admiration or indignation: " Puh-leeze! "
(15) Although the mutations lie far outside PC, they cause PC to be inactive, apparently because binding of RNA polymerase to the PUH promoter blocks the overlapping PC site.
(16) Length of implantation has varied from 1 to 48 days in the PUH series.
(17) From a genomic cosmid library of R. viridis constructed in the vector pHC79, clones that coded for subunits H (puh operon), L, M and cytochrome c (puf operon) of the photosynthetic reaction center were isolated and characterized.
(18) During this shift in growth conditions, the puf and puh mRNAs, coding for structural polypeptides of the photosynthetic apparatus, underwent a six- to eightfold induction, while the amount of mRNA from the fbc locus remained constant.
Pus
Definition:
(a.) The yellowish white opaque creamy matter produced by the process of suppuration. It consists of innumerable white nucleated cells floating in a clear liquid.
Example Sentences:
(1) It is concluded that ultrasonography, 67Ga scanning, and CT each have significant limits in diagnosing intra-abdominal pus.
(2) It is important that the nurse recognize when pus is a major factor in an unhealed wound and initiate local care to assist in cleaning the wound bed.
(3) Confirmation of diagnosis was established by exteriorization of pus with US, CT or during surgery.
(4) We isolated a strain of P. penneri from the pus of a patient with suppurative otitis media and an epidural abscess on June 10 and 15, 1989.
(5) Furthermore, useful antibacterial concentrations of both drugs were found in pus, sputum, and middle-ear fluid.
(6) The surgeons were able to aspirate the accumulated pus quite easily in 8 of the 9 patients with AIDS who underwent only intercostal drainage.
(7) Craniotomy disclosed an abscess containing yellow pus from which Streptococcus viridans was cultured.
(8) In the case of the suppurative reaction, pus drained along a root surface, destroying the periodontal ligament and interradicular bone until it emerged at the gingival sulcus.
(9) The final diagnosis was based on direct microscopy (2) or culture (1) of drained pus in the empyema cases and on histologic examination of resected tissue in the others.
(10) The mastoid cavity was found to be filled with pus and cholesteatoma debris.
(11) No macroscopic infection with pus formation occurred, while Micrococcus varians was cultured from each inoculated implant.
(12) When distribution of these organisms were classified depending on clinical materials from which they were isolated, outpatient sources from which S. aureus were isolated at high frequencies were otorrhea and pus, while inpatient sources with high incidents of S. aureus isolation were sputum and pus.
(13) No viability loss of B. fragilis was noted when pus was stored at 25 degrees C. Only slight loss of viaability of B. fragilis was observed at 15 degrees C. Escherichia coli coexisting in pus with B. fragilis increased several 100fold in 24 h when stored at 25 degrees C, but no significant growth occurred when they were kept at 15 degrees C. Approximately 20 to 40% of E. coli lost their viability when such pus was stored at 4 degrees C. We suggest that 15 degrees C may be an alternative temperature for storage of anaerobic specimens in laboratories where some delay in routine processing is unavoidable.
(14) The drug was not degraded by pus containing beta-lactamase and had equally good or better activity than nafcillin or vancomycin against Staphylococcus aureus or Staphylococcus epidermidis in vitro and in vivo.
(15) Pathogenic gram-negative bacilli and gram-positive pus-producing cocci are responsible for the studied pathology.
(16) aureus (in throat swabs and pus specimens), and enterobacteria were found.
(17) Bilateral tonsils were swollen, and covered with pus.
(18) Microflora isolated from cattle with acute postnatal pus-catarrhal endometritis has been studied.
(19) By combined gas chromatography and mass spectrometry the fatty acids of pus in patients with psoriasis pustulosa palmo-plantaris were analysed.
(20) Culture of aspirated pus revealed colonies of gram-positive cocci which were subsequently identified as E. faecalis.