(1) Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes.
(2) Mung bean nuclease protection analysis shows that the puh transcripts have different 5' termini within open reading frame G115 and a unique rho-independent termination signal within open reading frame I2372.
(3) The occurrence of overlapping transcripts for the puf and puh operons was further shown to influence development of the photochemical apparatus during conditions of environmental shifts in oxygen tension.
(4) These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely.
(5) Gene puh itself is transcribed as two mRNAs of 1118 and 1032 nucleotides.
(6) Open reading frame G115, upstream of structural gene puh, is transcribed as a 2.25-kilobase mRNA.
(7) The lifetimes of the puh messages, as determined by an oxygen blockade of transcription, were 10 and 12 min for the large and small puh mRNAs, respectively.
(8) In addition, the location and relative distance observed between the puf and puh operons which encode for light harvesting and reaction-centre structural genes are also conserved between these species.
(9) The mRNA transcripts of Rhodospirillum rubrum gene puh, coding for the H subunit of the photoreaction center, and of genes flanking puh were analyzed by blot hybridization.
(10) The Rhodospirillum rubrum structural gene puh, coding for the photoreaction center H polypeptide, and three other putative genes that surround puh were cloned and sequenced.
(11) In this study we demonstrate that the puh operon, which is located 39 kb away from the puf operon, also contains overlapping transcripts.
(12) Thus, in the mutants, RNA polymerase bound at the strong (mutant) PUH site effectively repress the PC promoter.
(13) Wave forms were selected from BAEPs performed in the last four years at the University of Pittsburgh Presbyterian University Hospital (PUH).
(14) His scattergun fluency is still punctuated by almost comically exaggerated displays of admiration or indignation: " Puh-leeze! "
(15) Although the mutations lie far outside PC, they cause PC to be inactive, apparently because binding of RNA polymerase to the PUH promoter blocks the overlapping PC site.
(16) Length of implantation has varied from 1 to 48 days in the PUH series.
(17) From a genomic cosmid library of R. viridis constructed in the vector pHC79, clones that coded for subunits H (puh operon), L, M and cytochrome c (puf operon) of the photosynthetic reaction center were isolated and characterized.
(18) During this shift in growth conditions, the puf and puh mRNAs, coding for structural polypeptides of the photosynthetic apparatus, underwent a six- to eightfold induction, while the amount of mRNA from the fbc locus remained constant.
Push
Definition:
(n.) A pustule; a pimple.
(v. t.) To press against with force; to drive or impel by pressure; to endeavor to drive by steady pressure, without striking; -- opposed to draw.
(v. t.) To thrust the points of the horns against; to gore.
(v. t.) To press or urge forward; to drive; to push an objection too far.
(v. t.) To bear hard upon; to perplex; to embarrass.
(v. t.) To importune; to press with solicitation; to tease.
(v. i.) To make a thrust; to shove; as, to push with the horns or with a sword.
(v. i.) To make an advance, attack, or effort; to be energetic; as, a man must push in order to succeed.
(v. i.) To burst pot, as a bud or shoot.
(n.) A thrust with a pointed instrument, or with the end of a thing.
(n.) Any thrust. pressure, impulse, or force, or force applied; a shove; as, to give the ball the first push.
(n.) An assault or attack; an effort; an attempt; hence, the time or occasion for action.
(n.) The faculty of overcoming obstacles; aggressive energy; as, he has push, or he has no push.
Example Sentences:
(1) The former Stoke City manager Pulis had reportedly been left frustrated by the club failing to push through deals for various players he targeted to strengthen the Palace squad.
(2) "It seems that this is just a few experts who are pushing it through parliament … without anyone thinking through the likely consequences for our country," said Duke Tagoe of the Food Sovereignty campaign group.
(3) John Large, a leading nuclear consultant, said: "The HSE as an independent agency will come under tremendous pressure to push through these designs.
(4) One might expect that a similar news spike and rebounding of support for stricter gun control can happen, given President Obama's new push.
(5) Activists in the country are pushing to get their voices heard ahead of Sunday's race.
(6) But late last month, Amisom pushed them out of Afgoye, a strategic stronghold 30km from Mogadishu, where Amisom officials say the militants used to manufacture explosives used in attacks on the capital.
(7) Gerhard Schröder , Merkel’s immediate predecessor, had pushed through parliament a radical reform agenda to get the country’s spluttering economy back on track.
(8) The view that testes found lateral to the external ring and which could be pushed some way into the scrotum were merely retractile was questioned.
(9) There’s a fine line between pushing them to their limits and avoiding injury, and Alberto is a master at it.
(10) The BBA statistics director, David Dooks, said: "It was no surprise to see the January mortgage figures falling back from December, when transactions were being pushed through to beat the end of stamp duty relief.
(11) Markets reacted calmly on Friday to the downgrade by Moody's of 16 European and US banks, with share prices steady after the reduction in credit ratings, which can push up the cost of borrowing for banks which they could pass on to customers.
(12) They also had speakers, long before boomboxes and mobile phones pushed sounds out in public.
(13) The minister for health, Mamy Lalatiana Andriamanarivo, says he is determined to push ahead with ambitious plans for universal free healthcare.
(14) The effect of 5 beta- and 5 alpha-reduced progestins on luteinizing hormone-releasing hormone (LH-RH) release was examined using either an in vitro superfusion or an in vivo push-pull perfusion (PPP) technique.
(15) That may well be the case, but it is extremely unlikely that Britain would be able to choose the terms of its future cooperation with the EU and not face push-back from member states.
(16) He can appoint Garland to the supreme court, and even push through the other 58 federal judicial nominees that are pending.
(17) The environment secretary, Liz Truss , has stripped farmers of subsidies for solar farms, saying they are a “blight” that was pushing food production overseas.
(18) Threadneedle Street has shaved 0.75 points off borrowing costs in but has not moved since April and with rising energy bills likely to push inflation close to 5% in the coming months is thought more likely to raise bank rate than cut it when the Bank meets this week.
(19) On physical examination the patients complained of pain on both passive flexion and internal rotation of the hip, and when the thigh was pushed backwards at 90 degrees of flexion.
(20) The ACT’s opposition leader, Jeremy Hanson, said during Tuesday’s debate that the uncertainty surrounding the new same-sex marriage regime created significant problems for couples, and he suggested the territory could be liable to compensation if it pushed ahead of the tolerance of the commonwealth, rather than waiting for the legalities to be settled.