(1) Four genes essential for bacteriochlorophyll biosynthesis were known to be encoded within a 45 kb region of the Rhodobacter sphaeroides genome, the boundaries of which are defined by puh and puf genes for reaction-centre and light-harvesting LH1 complexes.
(2) Mung bean nuclease protection analysis shows that the puh transcripts have different 5' termini within open reading frame G115 and a unique rho-independent termination signal within open reading frame I2372.
(3) The occurrence of overlapping transcripts for the puf and puh operons was further shown to influence development of the photochemical apparatus during conditions of environmental shifts in oxygen tension.
(4) These mutations improve the hutUH promoter (PUH) by changing the -10 region to match the consensus sequence more closely.
(5) Gene puh itself is transcribed as two mRNAs of 1118 and 1032 nucleotides.
(6) Open reading frame G115, upstream of structural gene puh, is transcribed as a 2.25-kilobase mRNA.
(7) The lifetimes of the puh messages, as determined by an oxygen blockade of transcription, were 10 and 12 min for the large and small puh mRNAs, respectively.
(8) In addition, the location and relative distance observed between the puf and puh operons which encode for light harvesting and reaction-centre structural genes are also conserved between these species.
(9) The mRNA transcripts of Rhodospirillum rubrum gene puh, coding for the H subunit of the photoreaction center, and of genes flanking puh were analyzed by blot hybridization.
(10) The Rhodospirillum rubrum structural gene puh, coding for the photoreaction center H polypeptide, and three other putative genes that surround puh were cloned and sequenced.
(11) In this study we demonstrate that the puh operon, which is located 39 kb away from the puf operon, also contains overlapping transcripts.
(12) Thus, in the mutants, RNA polymerase bound at the strong (mutant) PUH site effectively repress the PC promoter.
(13) Wave forms were selected from BAEPs performed in the last four years at the University of Pittsburgh Presbyterian University Hospital (PUH).
(14) His scattergun fluency is still punctuated by almost comically exaggerated displays of admiration or indignation: " Puh-leeze! "
(15) Although the mutations lie far outside PC, they cause PC to be inactive, apparently because binding of RNA polymerase to the PUH promoter blocks the overlapping PC site.
(16) Length of implantation has varied from 1 to 48 days in the PUH series.
(17) From a genomic cosmid library of R. viridis constructed in the vector pHC79, clones that coded for subunits H (puh operon), L, M and cytochrome c (puf operon) of the photosynthetic reaction center were isolated and characterized.
(18) During this shift in growth conditions, the puf and puh mRNAs, coding for structural polypeptides of the photosynthetic apparatus, underwent a six- to eightfold induction, while the amount of mRNA from the fbc locus remained constant.
Puy
Definition:
(n.) See Poy.
Example Sentences:
(1) A study of the prevalence of adult asthma (subjects over 18 years of age) has been carried out in the Puy-de-Dôme area over the past two years by means of a questionnaire sent out to all the doctors in the area.
(2) There's a world of difference between, say, the massive umami hit of one of my favourite comfort foods, a miso, Marmite and puy lentil vegetarian shepherd's pie, and the delicate crunch of a green salad.
(3) During a 3-year period, 93 prenatal diagnoses of kidney or urinary tract abnormalities were carried out in the French district of Puy-de-Dôme.
(4) By 1987, with 12 members, it had shifted to the central French village of Puy de Dôme and in 2004 to Germany , where it has been ever since.
(5) autodidactress, via GuardianWitness Serves 6 135ml olive oil 30g flour 2½ tsp salt 3kg oxtail 2 large carrots, cut into chunky batons 1 large celery stick, cut into chunky batons, plus a small bunch of celery leaves, roughly chopped 1 large onion, peeled and diced 2 garlic cloves, coarsely chopped 1 tsp black peppercorns 3 bay leaves 500ml beef stock 625ml Guinness or other stout 50g tomato puree 2 tsp sugar Long curl of fresh orange zest (optional) 200g puy lentils 1 Heat 90ml of the oil in a large, heavy-based pan over a medium flame.
(6) All infants born in the Puy-de-Dôme area in 1983 and referred to a neonatal care unit were studied prospectively.
(7) Serves 4 sunflower oil enough for frying John Dory fillets 4 x 180g (with the skin left on) salt and freshly ground pepper cooking chorizo 200g, cut into ½ cm dice curly kale 200g flat leaf parsley ½ bunch, leaves and stalks, finely chopped lemon juice of ½ For the lentils extra virgin olive oil 40ml onion 1, finely diced garlic 2 cloves thyme 2 sprigs rosemary 2 sprigs, leaves removed and chopped puy lentils 250g (if you have time, it’s good to soak them for an hour beforehand) chicken or vegetable stock 1 litre (a good quality cube will do) unsalted butter 30g To cook the lentils, pour olive oil into a heavy-bottomed saucepan and gently cook.
(8) Serves 4 extra-virgin olive oil 4 tbsp brown onion 1, chopped garlic cloves 4, finely chopped small beetroot 8, peeled and quartered carrots 6, sliced into large pieces bay leaves 3 thyme 2 sprigs sea salt and freshly ground pepper tomato purée 2 tbsp red wine (use vegan wine if you like) 250ml vegetable stock 500ml puy lentils 400g portobello mushrooms 2-3, sliced crimini mushrooms 10 pearl onions 10, peeled arrowroot 2 tsp, dissolved in 2 tbsp water thyme a few sprigs, to garnish Heat 2 tablespoons of the olive oil in a thick-bottomed saucepan over a medium heat.
(9) The earthiness of the curly kale and puy lentils creates a good balance for this autumnal dish.
(10) Oxtail braised in black beer with puy lentils You will need a deep, heavy saucepan for this one.
(11) Later, two studies were performed in adults, in two places of Haute-Loire and Puy-de-Dôme, respectively Arsac-en-Velay and Besse-en-Chandesse.
(12) Serves 4 6 tbs olive oil 4 garlic cloves, finely chopped 2 rounded tsp cumin seeds, roughly ground 125g fresh coriander, chopped and stalks set aside 250g small brown or puy lentils 1.75 litres water a squeeze of lemon sea salt and black pepper For the caramelised onions: 300ml sunflower oil 2 large onions, halved and sliced into rounds as thin and even as possible (we recommend using a mandolin) To make the caramelised onions, heat the oil in a large saucepan until very hot.