(1) The previously uncharacterized third and fourth genes (pulE and pulF) of the pullulanase secretion gene operon of Klebsiella oxytoca strain UNF5023 are, respectively, predicted to encode a 55 kDa polypeptide with a putative nucleotide-binding site, and a highly hydrophobic 44 kDa polypeptide that probably spans the cytoplasmic membrane several times.
(2) Under basal conditions oral penbutolol induced a decrease of pule rate and blood pressure but no change in plasma or urinary catecholamines.
(3) This suppression was due to increased [K+]0 and not to K-induced depolarization because it persisted when membrane potential was held by means of a conditioning hyperpolarizing puled gradually after maximum repolarization.
(4) 25 laryngeal carcinomas were investigated by pules cytophotometry, stained with ethidium bromide after pepsination.
(5) These results are in line with the predicted absence from PulE of a region of sufficient hydrophobicity to function as a signal sequence.
(6) Expression of pulE in minicells or under the control of a strong bacteriophage T7 promoter resulted in the production of a c. 58 kDa cytoplasmic protein.
(7) Gene disruption experiments indicated that both pulE and pulF are required for pullulanase secretion in Escherichia coli K-12.
(8) A representative PulE-beta-galactosidase hybrid protein created by Tnlac mutagenesis was also found mainly in the cytoplasm.
(9) These include the P. aeruginosa PilB protein, the ComG ORF-1 protein from the Bacillus subtilis comG operon (necessary for competence), the PulE protein from the Klebsiella oxytoca (formerly K. pneumoniae) pulC-O operon (involved in pullulanase export), and the VirB-11 protein from the virB operon (involved in virulence) which is located on the Agrobacterium tumefaciens Ti plasmid.
(10) They issue great puling statements about income imbalance in a game that pays them $100m per annum just for the act of being able to cash checks and maybe pay attention long enough to run a franchise into the ground.
(11) In addition, PulE protein has consensus sequences found in a wide variety of nucleotide-binding proteins.
(12) The deduced amino acid sequence of ORF1 is related to the Klebsiella pneumoniae PulE protein, to the Bacillus subtilis ComG ORF1 and to the Agrobacterium tumefaciens VirB ORF11 products.
Pulled
Definition:
(imp. & p. p.) of Pull
(a.) Plucked; pilled; moulting.
Example Sentences:
(1) "I pulled the microphone in front of my seat, not a knife.
(2) Critics say he is unelectable as prime minister and will never be able to implement his plans, but he has nonetheless pulled attention back to an issue that many thought had gone away for good.
(3) It pulled to a halt and a bodyguard got out and knocked me unconscious.
(4) The visitors did have a chance to pull another back with three minutes remaining but Henry blazed a free-kick from within range on the left over the bar, summing up Wolves’ day out in the East Midlands.
(5) Nango's dwellings are built on skis so can be pulled around the beach, and have a glass roof to view the northern lights.
(6) The effect of 5 beta- and 5 alpha-reduced progestins on luteinizing hormone-releasing hormone (LH-RH) release was examined using either an in vitro superfusion or an in vivo push-pull perfusion (PPP) technique.
(7) The person responsible for pulling the trigger was equally likely to be a friend, a family member, or the victim.
(8) The cull in 2013 required a policing effort costing millions of pounds and pulling in officers from many different forces.
(9) Asymmetries occur less often whilst using the low-cervical-pull according to Sander, due to the reduced friction between the two plastic parts of this headgear system.
(10) Harvest the bulbs once they reach 7-8cm across; if you cut them off at ground level rather than pulling the whole plant up, the roots should produce a second crop of feathery shoots.
(11) Eight macerated human child skulls with a dental age of approximately 9.5 years (mixed dentition) were consecutively subjected to an experimental standardized high-pull headgear traction system attached to the maxilla at the first permanent molar area via an immovable acrylic resin splint covering all teeth.
(12) All the others, all that bullshit, they just want to pull me down from the top but I will not go.
(13) Even the landscape is secretive: vast tracts of crown land and hidden valleys with nothing but a dead end road and lonely farmhouse, with a tractor and trailer pulled across the farmyard for protection.
(14) A Zliten hospital spokesman told Associated Press that 60 bodies had been pulled from the wreckage, though Fozi Awnais, from the health ministry in Tripoli, later said 47 people had died and 118 more were injured.
(15) "The rise in those who are self-employed is good news, but the reality is that those who have turned to freelance work in order to pull themselves out of unemployment and those who have decided to work for themselves face a challenging tax maze that could land them in hot water should they get it wrong," says Chas Roy-Chowdhury, head of taxation at the Association of Certified Chartered Accountants.
(16) Last week, Cohen estimated the militants were still earning “several million dollars per week from the sale of stolen and smuggled energy resources” – down on what they pulled in before the coalition air strikes, but still a substantial amount.
(17) The comedian Daniel O’Reilly, who gives laddish advice on how to “pull birds” under the guise of a deliberately provocative character in the ITV2 series, has proved controversial for lines such as “Just show her your penis.
(18) The second national multiplex was handed to 4 Digital, but was handed back after Channel 4 pulled out.
(19) AJ Green was waiting just behind him, and the receiver gratefully pulled in the softly fluttering ball.
(20) By simultaneously pushing the foot bar and pulling the hand bar, the monkey lifts a weight and triggers a microswitch which releases a banana-flavored food pellet into a well close to the animal's mouth.