(n.) The act of purifying; the act or operation of separating and removing from anything that which is impure or noxious, or heterogeneous or foreign to it; as, the purification of liquors, or of metals.
(n.) The act or operation of cleansing ceremonially, by removing any pollution or defilement.
(n.) A cleansing from guilt or the pollution of sin; the extinction of sinful desires, appetites, and inclinations.
Example Sentences:
(1) During enzyme purification two nucleases were identified.
(2) Change of steps in achieved just by varying the reaction conditions without any product purification.
(3) Further purification of ZAB by filtration through Sephadex G-100 gave a preparation (ZAB2) which contained the common antigen as shown by the cross-reactivity of anti-ZAB2 rat serum with seven stains of N. gonorrhoeae.
(4) Stable factor-dependent B-cell hybridomas were used to monitor the purification of the growth factor from the supernatant of a clonotypically stimulated mouse helper T-cell clone.
(5) After immunoadsorbent purification, the final step in a purification procedure similar to that adopted for colon cancer CEA, two main molecular species were identified: 1) Material identical with colon cancer CEA with respect to molecular size, PCA solubility, ability to bind to Con A, and most important the ability to bind to specific monkey anti-CEA serum.
(6) Currently there are no IOC approved definitive tests for these hormones but highly specific immunoassays combined with suitable purification techniques may be sufficient to warrant IOC approval.
(7) A rapid method is described for the purification and analysis of synthetic oligonucleotides, based on reversed-phase high-performance liquid chromatography.
(8) The influence of exogenous mitogens (RFG, TGF beta 1 and insulin) and autocrine factor (at different step of purification) on the growth of Morris hepatoma 7777 (MH) cells was estimated by both methods.
(9) In a previous publication the purification and properties of two protein kinases (KI and KII) from a soluble fraction of bovine corpus luteum and the stimulation of the latter fol.
(10) for 48 h followed by Leydig cell isolation and purification resulted in a decrease in the maxima of hCG-induced cAMP accumulation and testosterone production by approximately 70% and approximately 55%, respectively, when compared to cells of control mice.
(11) These plasmids allow expression of native or truncated forms of the enzyme and easy purification of the products.
(12) The enzyme extracted from strains containing the recombinant plasmid was identical to N. crassa catabolic dehydroquinase by the criteria of heat stability, ammonium sulfate fractionation, immunological crossreactivity, molecular weight, and purification characteristics.
(13) Three triacetinases (A, B and C) were shown to undergo reciprocal conversions under storage and during some purification procedures (effect of pH, ionic strength, ion-exchange chromatography, concentration, lyophilization, etc.).
(14) Sindbis virus nucleocapsids were isolated from mature virions by a two-step purification method.
(15) The major scrapie prion protein, designated PrP 27-30, exhibited both charge and size heterogeneity after purification from infected hamster brains.
(16) We have used this procedure successfully during the purification of epidermal glycoproteins.
(17) The purification and concentration of these viruses in their monomeric forms is hazardous when conventional "tube" rotors are used since they invariably result in dissociation and aggregation of the virus particles.
(18) These methods can be applied to the purification and characterization of the as yet undefined secretory and circulating forms of PTHrP.
(19) The possibility that mammalian DNA topoisomerase II is an intracellular target which mediates drug-induced DNA breaks is supported by the following studies using 4'-(9-acridinylamino)methane-sulfon-m-anisidide (m-AMSA): (a) a single m-AMSA-dependent DNA cleavage activity copurified with calf thymus DNA topoisomerase II activity at all chromatographic steps of the enzyme purification; (b) m-AMSA-induced DNA cleavage by this purified activity resulted in the covalent attachment of protein to the 5'-ends of the DNA via a tyrosyl phosphate bond.
(20) These experiments may provide the basis for the expanded use of immobilized lectins for purification and characterization of hydrolases and other glycoproteins.
Recrystallize
Definition:
(v. i. & t.) To crystallize again.
Example Sentences:
(1) The recrystallized surface protein arrays gave a resolution of approximately 1.3 nm in projection after correlation averaging of negatively stained preparations.
(2) A synthetic precursor to a potent dopaminergic agonist, (RS)-4-hydroxy-2-(di-n-propylamino)indan, has been resolved by classical recrystallization procedures, and the absolute configurations of the enantiomers have been established by X-ray crystallographic analysis.
(3) Pure alpha-epimer was obtained by recrystallization and low pressure silica gel column chromatography combined with HPLC method.
(4) Microsomal and low speed pellets with added cofactors formed products which recrystallized with either etiocholanolone or androsterone from [3H]T or [3H]DHT, respectively.
(5) The blood-brain barrier in the rhesus monkey was opened to intravascular Evans blue-albumin, without causing brain edema or altering brain electrolytes, by perfusing 2.5 molal recrystallized D,L-lactamide into the internal carotid artery for 20--30 sec.
(6) Electrophoresis, microscopical observation and pH dependency of encapsulability of recrystallized cores revealed that the electrostatic attractive force between gelatin molecules in solution and a polymer attached to the core particle cause gelatin adsorption on the core surface to result in a great improvement in encapsulability.
(7) The porosity in lased enamel was overall very similar to that seen in enamel heated in an electric furnace to a temperature of 600 degrees C. The presence of recrystallized enamel crystals indicated a temperature rise of approximately 1000 degrees C and the grain growth indicated that a temperature > or = 1000 degrees C existed for some time after the laser irradiation.
(8) Products of cholesterol auto-oxidation were concentrated from several lots of USP-grade cholesterol by recrystallizing cholesterol from the methanol extract, retaining the mother liquor, and evaporating the residuum to dryness under vacuum.
(9) Recrystallization of the synthetic product produced material identical in all respects with natural PGE1.
(10) Experiments showed that sulphadimidine crystals recrystallized from ethanol had better dissolution and absorption characteristics than sulphadimidine crystals recrystallized from methanol.
(11) Thermal studies (DSC and HSM) showed that form II melts at 196 degrees C, while form I melts at 193 degrees C, immediately followed by a resolidification and remelt at 196 degrees C. The conversion of form II to form I was accomplished by recrystallization from ethanol or methanol, and the form I-to-form II transition was obtained by controlled heating of form I around 194 degrees C. Quantitative XRD was used to determine the polymorphic composition, with a detection limit of less than 1% of the minor form and a linearity of 0-10% form I in form II (correlation coefficient of 0.999).
(12) Microcapsule batch reproducibility depended mainly on the variation in particle size distribution of the recrystallized core material.
(13) After the administration of 3-H-testosterone to castrated hamsters, extracts from crude nuclei were separated into bound and free fractions by gel filtration of Sephadex G-25, Subsequent analysis by thin-layer chromatography and recrystallization showed that dihydrotestosterone was the predominant radioactive steroid in the bound fraction and that it increased steadily with time until 1 hr after the injection.
(14) 1,2-Dimethyl-3-hydroxy-4-pyridone (1), a crystalline oral iron chelator, forms an acetic acid solvate (2) on recrystallization from acetic acid and carbon tetrachloride.
(15) Recrystallization of the purified enzyme under different conditions readily produces a variety of single crystals.
(16) Ice recrystallization was practically absent at the temperature normally used in cryo-sectioning (-70 degrees C).
(17) A convenient, detailed procedure is given for the recrystallization of commercial dithionite from 0.1 M NaOH-methanol under anaerobic conditions.
(18) Thus, the protein penetrates partway into the bilayer, distorts the packing of the lipid fatty acid chains, and prevents recrystallization, thus decreasing the phase transition temperature.
(19) Winter stem fluid from the bittersweet nightshade, Solanum dulcamara L., also showed the recrystallization inhibition activity characteristic of the animal thermal hysteresis proteins (THPs), suggesting a possible function for the THPs in this freeze tolerant species.
(20) The chimeric proteins varied in their effectiveness of inhibiting ice recrystallization according to the number of 11-amino acid repeats present in the antifreeze moiety.