What's the difference between purified and purifier?

Purified


Definition:

  • (imp. & p. p.) of Purify

Example Sentences:

  • (1) Competition with the labelled 10B12 MAb for binding to the purified antigen was demonstrated in sera of tumor-bearing and immune rats.
  • (2) Previous attempts to purify this enzyme from the liquid endosperm of kernels of Zea mays (sweet corn) were not entirely successful owing to the lability of partially purified preparations during column chromatography.
  • (3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
  • (4) The quaternary structure of ribonucleotide reductase of Escherichia coli was investigated, with the use of purified B1 and B2 proteins and bifunctional cross-linking agents.
  • (5) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
  • (6) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
  • (7) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
  • (8) The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei.
  • (9) heterografts of GW-39, a CEA-producing colonic tumor of human origin, was demonstrated in radioimmunoassay using radioiodinated CEA purified from GW-39.
  • (10) Four new monochain phospholipases were purified from the Oxyuranus scutellatus (taipan) venom.
  • (11) By growing purified human cytotrophoblasts under serum-free conditions and manipulating the culture surface, we were able to disassociate morphologic from biochemical differentiation.
  • (12) Lysates of cells were compared to purified DNA as PCR template.
  • (13) Histone mRNA, labeled with 32P or 3H-methionine during the S phase of partially synchronized HeLa cells, was isolated from the polyribosomes and purified as a "9S" component by sucrose gradient sedimentation.
  • (14) Proliferation of quiescent hematopoietic stem cells, purified by cell sorting and evaluated by spleen colony assay (CFU-S), was investigated by measuring the total cell number and CFU-S content and the DNA histogram at 20 and 48 hours of liquid culture.
  • (15) Adult nonpregnant female rhesus monkeys fed purified diets containing 100 or 4 ppm zinc for 1 yr were mated then studied through midgestation.
  • (16) Monoclonal antibodies (mAbs) against the soluble form (S-COMT) of catechol-O-methyltransferase (COMT, EC 2.1.1.6) were produced using a purified preparation of the enzyme from pig liver as antigen.
  • (17) Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo.
  • (18) The specificity of the assay was established by competitive displacement of 125I-labeled arginine-rich protein from its antiserum by arginine-rich protein and lipoproteins containing this protein, but not by rat albumin or other purified apolipoproteins.
  • (19) To augment the in vitro expansion of LAK cells, we added highly purified human recombinant interleukin-2, phytohemagglutinin and accessory cells (Uc cells) to the LAK culture system, with which huge number of LAK cells (LAK-L) were generated from originally small number of peripheral blood lymphocytes of cancer patients.
  • (20) This enzyme was purified to homogeneity and exhibited an apparent molecular weight of 36,000 on sodium dodecyl sulfate gels and 180,000 on a TSK G-3000SW column in the presence of Triton X-100.

Purifier


Definition:

  • (n.) One who, or that which, purifies or cleanses; a cleanser; a refiner.

Example Sentences:

  • (1) Competition with the labelled 10B12 MAb for binding to the purified antigen was demonstrated in sera of tumor-bearing and immune rats.
  • (2) Previous attempts to purify this enzyme from the liquid endosperm of kernels of Zea mays (sweet corn) were not entirely successful owing to the lability of partially purified preparations during column chromatography.
  • (3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
  • (4) The quaternary structure of ribonucleotide reductase of Escherichia coli was investigated, with the use of purified B1 and B2 proteins and bifunctional cross-linking agents.
  • (5) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
  • (6) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
  • (7) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
  • (8) The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei.
  • (9) heterografts of GW-39, a CEA-producing colonic tumor of human origin, was demonstrated in radioimmunoassay using radioiodinated CEA purified from GW-39.
  • (10) Four new monochain phospholipases were purified from the Oxyuranus scutellatus (taipan) venom.
  • (11) By growing purified human cytotrophoblasts under serum-free conditions and manipulating the culture surface, we were able to disassociate morphologic from biochemical differentiation.
  • (12) Lysates of cells were compared to purified DNA as PCR template.
  • (13) Histone mRNA, labeled with 32P or 3H-methionine during the S phase of partially synchronized HeLa cells, was isolated from the polyribosomes and purified as a "9S" component by sucrose gradient sedimentation.
  • (14) Proliferation of quiescent hematopoietic stem cells, purified by cell sorting and evaluated by spleen colony assay (CFU-S), was investigated by measuring the total cell number and CFU-S content and the DNA histogram at 20 and 48 hours of liquid culture.
  • (15) Adult nonpregnant female rhesus monkeys fed purified diets containing 100 or 4 ppm zinc for 1 yr were mated then studied through midgestation.
  • (16) Monoclonal antibodies (mAbs) against the soluble form (S-COMT) of catechol-O-methyltransferase (COMT, EC 2.1.1.6) were produced using a purified preparation of the enzyme from pig liver as antigen.
  • (17) Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo.
  • (18) The specificity of the assay was established by competitive displacement of 125I-labeled arginine-rich protein from its antiserum by arginine-rich protein and lipoproteins containing this protein, but not by rat albumin or other purified apolipoproteins.
  • (19) To augment the in vitro expansion of LAK cells, we added highly purified human recombinant interleukin-2, phytohemagglutinin and accessory cells (Uc cells) to the LAK culture system, with which huge number of LAK cells (LAK-L) were generated from originally small number of peripheral blood lymphocytes of cancer patients.
  • (20) This enzyme was purified to homogeneity and exhibited an apparent molecular weight of 36,000 on sodium dodecyl sulfate gels and 180,000 on a TSK G-3000SW column in the presence of Triton X-100.

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