(1) Competition with the labelled 10B12 MAb for binding to the purified antigen was demonstrated in sera of tumor-bearing and immune rats.
(2) Previous attempts to purify this enzyme from the liquid endosperm of kernels of Zea mays (sweet corn) were not entirely successful owing to the lability of partially purified preparations during column chromatography.
(3) The nuclear origin of the Ha antigen was confirmed by the speckled nuclear immunofluorescence staining pattern given by purified antibody to Ha obtained from a specific immune precipitate.
(4) The quaternary structure of ribonucleotide reductase of Escherichia coli was investigated, with the use of purified B1 and B2 proteins and bifunctional cross-linking agents.
(5) Western blot analysis of these mitochondria using an antibody against carnitine palmitoyltransferase II purified from beef heart demonstrates a 68-kDa protein, which under ischemic conditions apparently is decreased by 2 kDa.
(6) ASF-II was purified to apparent homogeneity by using concanavalin A-agarose affinity chromatography, DEAE-cellulose chromatography, alumina gel adsorption, and isoelectric focussing techniques.
(7) The enzyme was solubilized by Triton X-100 and purified approximately 480-fold by gel filtration and affinity chromatography on alanine methyl ketone-AH-Sepharose 4B.
(8) The DNA untwisting enzyme has been purified approximately 300-fold from rat liver nuclei.
(9) heterografts of GW-39, a CEA-producing colonic tumor of human origin, was demonstrated in radioimmunoassay using radioiodinated CEA purified from GW-39.
(10) Four new monochain phospholipases were purified from the Oxyuranus scutellatus (taipan) venom.
(11) By growing purified human cytotrophoblasts under serum-free conditions and manipulating the culture surface, we were able to disassociate morphologic from biochemical differentiation.
(12) Lysates of cells were compared to purified DNA as PCR template.
(13) Histone mRNA, labeled with 32P or 3H-methionine during the S phase of partially synchronized HeLa cells, was isolated from the polyribosomes and purified as a "9S" component by sucrose gradient sedimentation.
(14) Proliferation of quiescent hematopoietic stem cells, purified by cell sorting and evaluated by spleen colony assay (CFU-S), was investigated by measuring the total cell number and CFU-S content and the DNA histogram at 20 and 48 hours of liquid culture.
(15) Adult nonpregnant female rhesus monkeys fed purified diets containing 100 or 4 ppm zinc for 1 yr were mated then studied through midgestation.
(16) Monoclonal antibodies (mAbs) against the soluble form (S-COMT) of catechol-O-methyltransferase (COMT, EC 2.1.1.6) were produced using a purified preparation of the enzyme from pig liver as antigen.
(17) Transcription studies in vitro on repression of the tryptophan operon of Escherichia coli show that partially purified trp repressor binds specifically to DNA containing the trp operator with a repressor-operator dissociation constant of about 0.2 nM in 0.12 M salt at 37 degrees , a value consistent with the extent of trp operon regulation in vivo.
(18) The specificity of the assay was established by competitive displacement of 125I-labeled arginine-rich protein from its antiserum by arginine-rich protein and lipoproteins containing this protein, but not by rat albumin or other purified apolipoproteins.
(19) To augment the in vitro expansion of LAK cells, we added highly purified human recombinant interleukin-2, phytohemagglutinin and accessory cells (Uc cells) to the LAK culture system, with which huge number of LAK cells (LAK-L) were generated from originally small number of peripheral blood lymphocytes of cancer patients.
(20) This enzyme was purified to homogeneity and exhibited an apparent molecular weight of 36,000 on sodium dodecyl sulfate gels and 180,000 on a TSK G-3000SW column in the presence of Triton X-100.
Refined
Definition:
(imp. & p. p.) of Refine
(a.) Freed from impurities or alloy; purifed; polished; cultured; delicate; as; refined gold; refined language; refined sentiments.
Example Sentences:
(1) The patients had a high AP, consumed more alcohol, were more well-fed, older and consumed more refined carbohydrates per 1 kg bw and less cholesterol and vegetable protein.
(2) After restrained least-squares refinement of the enzyme-substrate complex with the riboflavin omitted from the model, additional electron density appeared near the pyrophosphate, which indicated the presence of an ADPR molecule in the FAD binding site of PHBH.
(3) Well-refined x-ray structures of the liganded forms of the wild-type and a mutant protein isolated from a strain defective in chemotaxis but fully competent in transport have provided a molecular view of the sugar-binding site and of a site for interacting with the Trg transmembrane signal transducer.
(4) To meet these prerequisites we have introduced some technical refinements: (1) computer-controlled rectilinear translations of the target in combination with different angular positions of the source and (2) computer-controlled rotations of the target around a vertical axis in combination with different angular positions of the source.
(5) Percutaneous transhepatic biliary drainage (PTBD) was conceptualized more than 35 years ago, but its clinical application only flourished in the past 10 years after a number of technical refinements.
(6) In 1984 the press-fit condylar knee was first introduced and was intended to provide a condylar knee system primarily for posterior cruciate retention that addressed refinements in metallurgy, prosthetic geometry and sizing, cementless fixation, inventory management, and instrumentation.
(7) Obviously, the sheer number of lasers being used both clinically and experimentally indicates a great potential for further advancement and refinement in technique and surgical outcomes.
(8) Phases from x-ray structure factors (R = 0.43) computed from this model were then used in the calculation of another electron density map against which the model was further refined.
(9) Staging classifications are being refined to reflect increasing knowledge of important prognostic indicators, e.g., absence or presence of lymph node involvement, pattern of lymph node involvement, and absence or presence of visceral disease.
(10) The ordered aspect of the genetic code table makes this result a plausible starting point for studies of the origin and evolution of the genetic code: these could include, besides a more refined optimization principle at the logical level, some effects more directly related to the physico-chemical context, and the construction of realistic models incorporating both aspects.
(11) The structure of Mn(III) superoxide dismutase (Mn(III)SOD) from Thermus thermophilus, a tetramer of chains 203 residues in length, has been refined by restrained least-squares methods.
(12) Based on the refined atomic coordinates of the tRNAphe in the orthorhombic crystal, on the recent advances in the distance dependence of the ring-current magnetic field effects and on the adopted values for the isolated hydrogen-bonded NH resonances, a computed spectrum consisting of 23 protons was constructed.
(13) It can be used as a simple screening procedure to help determine which of many possible anthelmintic control strategies should be selected for more detailed examination in the field, and it provides a theoretical framework within which ideas concerning the epidemiology of parasitic gastroenteritis can be assessed and refined.
(14) The advances in lid and orbital surgery are due to the improvements made in diagnostic equipment and to technical refinements.
(15) The group’s refining business performed better than expected, more than doubling profit to $2.2bn from $1bn.
(16) They also suggest that both the migration of cortical neurons on glia and the refinement of the mapping between the peripheral whisker field and its cortical representation may depend upon the distribution of substrate adhesion molecules.
(17) Thus the present study gives support for a protective effect associated with a fiber-rich or vegetable-rich diet, while it indicates that frequent consumption of refined starchy foods, eggs and fat-rich foods such as cheese and red meat is a risk factor for colo-rectal cancer.
(18) Synthesis and discussion is focused on five major areas in which gerontological continuity and change are evidenced: 1) transformation of basic themes over time; 2) gerontology's identity crisis; 3) the social ideology of gerontology; 4) evolution and refinement of gerontological ideas and methods; and 5) temporal frameworks.
(19) The course content and format were refined after 11 pharmacists completed a pilot program.
(20) This has led to important advances in our understanding of the mechanism of axonal guidance, the physiology of neurotrophic factors and the establishment and refinement of neural connections.