(1) Closed circular pYe(ARG4)411 DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.
(2) The substrates have been used in a spectrophotometric assay of PyEs and in a staining method to locate PyEs on polyacrylamide electrophoresis.
(3) "The bass really vibrates along the floorboards at Pye and one night we were in the middle of a take and it was really moving, going home.
(4) Deputy clerk of the Senate, Richard Pye, has kindly given a translation.
(5) Poets laureate were given sherry until Henry Pye, in 1790, relinquished his butt in exchange for a £27 yearly fee.
(6) Senior G4S personnel, including McCaffrey and centre manager Kevin Pye, who were present on both nights of violence, repeatedly denied that G4S had handed over management of the centre on the evening of 17 February, an allegation reported by ABC TV , based on a guard’s accounts.
(7) A hybrid ColE1 plasmid [pYe(trp5)1], containing a yeast DNA segment that complements auxotrophic point mutations and deletions in the Escherichia coli tryptophan synthetase gene (trpAB), has been isolated.
(8) Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322.
(9) Southern blotting assay of transformant chromosomal DNA indicates that reconstitution of ade2 defective gene is related with its "correction", owing to integration of pYE(ADE2)2 sequence in the vicinity of the mutant locus.
(10) A PYE UNICAM spectrophotometer was used, with KBr pellet technique.
(11) Extracts were evaporated to dryness, whereupon the residues were dissolved in 5 ml of acetone or petroleum ether, and were analysed by gas chromotography (series 104 Pye Unicam gas chromotograph fitted with ECD and TID detectors).
(12) MB • Aberdeen move to within a point of Celtic after comeback at Partick Thistle • Steven Pye: when Aberdeen ruled Scottish football Facebook Twitter Pinterest Mathias Pogba moved from Crawley Town to Partick Thistle last summer but has found goals hard to come by north of the border.
(13) In 1954 he married a teacher, Margaret Pye, from Rhodesia (now Zimbabwe) and moved with her to London.
(14) In particular the experimental work which has been modelled is that of Lanyon, Goodship, Pye and McFie.
(15) The red adenine-dependent mutants ade1 of the yeast Pichia pinus blocked in the VI step of adenine biosynthesis (lack of AIR-carboxylase) and ade2 mutants blocked in the VII step of adenine biosynthesis (lack of SAIKAR-synthase) were transformed with the plasmid pYE(ADE2)2 containing ADE2 gene of Saccharomyces cerevisiae encoding AIR-carboxylase.
(16) Red adenine-dependent mutants of Hansenula polymorpha, Pichia guilliermondii, Williopsis saturnus yeasts have been transformed by the plasmid pYE (ADE2) 2 DNA containing ADE2 gene from Saccharomyces cerevisiae.
(17) But the commission was careful to include a wide range of views, including Pye Jakobsson , who has worked in the Swedish sex industry for several years and has actively campaigned against the criminalisation of punters, and other pro-prostitution activists.
(18) The ionic concentrations were determined using a Pye Unicam SP atomic absorption spectrophotometer.
(19) Since yeast cells transformed by this plasmid poorly secreted RNase into the medium, the plasmid pYE RNAP-Rh was constructed, in which the signal sequence of RNase Rh was replaced by the prepro-sequence of aspartic proteinase-I, one of the extracellular enzymes secreted by R. niveus.
(20) Prepared standards of halothane in oxygen were analysed using a Pye Series 104 gas chromatograph equipped with a heated head flame ionization detector and gas sampling valve.