(1) Closed circular pYe(ARG4)411 DNA was detected in transformant DNA preparations by hybridization to pBR322 DNA and by transformation of E. coli to ampicillin resistance.
(2) The substrates have been used in a spectrophotometric assay of PyEs and in a staining method to locate PyEs on polyacrylamide electrophoresis.
(3) "The bass really vibrates along the floorboards at Pye and one night we were in the middle of a take and it was really moving, going home.
(4) Deputy clerk of the Senate, Richard Pye, has kindly given a translation.
(5) Poets laureate were given sherry until Henry Pye, in 1790, relinquished his butt in exchange for a £27 yearly fee.
(6) Senior G4S personnel, including McCaffrey and centre manager Kevin Pye, who were present on both nights of violence, repeatedly denied that G4S had handed over management of the centre on the evening of 17 February, an allegation reported by ABC TV , based on a guard’s accounts.
(7) A hybrid ColE1 plasmid [pYe(trp5)1], containing a yeast DNA segment that complements auxotrophic point mutations and deletions in the Escherichia coli tryptophan synthetase gene (trpAB), has been isolated.
(8) Saccharomyces cerevisiae, Candida maltosa and Pichia guilliermondii have been transformed by the plasmid pYe(ARG4)411, which contains the S. cerevisiae ARG4 gene inserted into pBR322.
(9) Southern blotting assay of transformant chromosomal DNA indicates that reconstitution of ade2 defective gene is related with its "correction", owing to integration of pYE(ADE2)2 sequence in the vicinity of the mutant locus.
(10) A PYE UNICAM spectrophotometer was used, with KBr pellet technique.
(11) Extracts were evaporated to dryness, whereupon the residues were dissolved in 5 ml of acetone or petroleum ether, and were analysed by gas chromotography (series 104 Pye Unicam gas chromotograph fitted with ECD and TID detectors).
(12) MB • Aberdeen move to within a point of Celtic after comeback at Partick Thistle • Steven Pye: when Aberdeen ruled Scottish football Facebook Twitter Pinterest Mathias Pogba moved from Crawley Town to Partick Thistle last summer but has found goals hard to come by north of the border.
(13) In 1954 he married a teacher, Margaret Pye, from Rhodesia (now Zimbabwe) and moved with her to London.
(14) In particular the experimental work which has been modelled is that of Lanyon, Goodship, Pye and McFie.
(15) The red adenine-dependent mutants ade1 of the yeast Pichia pinus blocked in the VI step of adenine biosynthesis (lack of AIR-carboxylase) and ade2 mutants blocked in the VII step of adenine biosynthesis (lack of SAIKAR-synthase) were transformed with the plasmid pYE(ADE2)2 containing ADE2 gene of Saccharomyces cerevisiae encoding AIR-carboxylase.
(16) Red adenine-dependent mutants of Hansenula polymorpha, Pichia guilliermondii, Williopsis saturnus yeasts have been transformed by the plasmid pYE (ADE2) 2 DNA containing ADE2 gene from Saccharomyces cerevisiae.
(17) But the commission was careful to include a wide range of views, including Pye Jakobsson , who has worked in the Swedish sex industry for several years and has actively campaigned against the criminalisation of punters, and other pro-prostitution activists.
(18) The ionic concentrations were determined using a Pye Unicam SP atomic absorption spectrophotometer.
(19) Since yeast cells transformed by this plasmid poorly secreted RNase into the medium, the plasmid pYE RNAP-Rh was constructed, in which the signal sequence of RNase Rh was replaced by the prepro-sequence of aspartic proteinase-I, one of the extracellular enzymes secreted by R. niveus.
(20) Prepared standards of halothane in oxygen were analysed using a Pye Series 104 gas chromatograph equipped with a heated head flame ionization detector and gas sampling valve.
Wye
Definition:
(n.) The letter Y.
(n.) A kind of crotch. See Y, n. (a).
Example Sentences:
(1) The results are broadly consistent with a more homogeneous environment for the Wye base in the presence of Mg2+.
(2) Then, in October 1998, as the newly appointed foreign minister, he astounded his acolytes by signing the Wye River agreement, facilitated in Maryland by President Bill Clinton, which granted Palestinians control over another 13% of the West Bank.
(3) After attending Rose Hill school, Tunbridge Wells, and Durham school, he dropped out of Wye Agricultural College in Kent in order to study acting at the Old Vic.
(4) They knew they had to go west, because the storm-troopers would be coming from the east; they got as far as Ross-on-Wye, where their passports and cameras were taken away.
(5) By way of escape, David headed for a hotel in Ross-on-Wye with an old lover, George Lassalle.
(6) An examination of the frameshift signals or proposed signals within published sequences of retroviruses and other genetic elements from higher animals shows that each site utilizes a tRNA which normally contains Wybutoxine (Wye) base or Queuine (Q) base in the anticodon loop.
(7) Work undertaken within the Wye basin, central Wales, demonstrates a complex distribution of fallout at the catchment scale, with the bulk of the deposition concentrated in a narrow north-south band, situated in the west central areas of the basin, which contained in excess of 1500 Bq m(-2) of (134)Cs.
(8) The complex conformational states of the anticodon loop of yeast tRNA(Phe) which we had previously studied with relaxation experiments by monitoring fluorescence of the naturally occurring Wye base, are analyzed using time and polarization resolved fluorescence measurements at varying counterion concentrations.
(9) Sky Arts has made a number of profile-raising deals, including sponsoring the Hay on Wye festival since 2007, backing English National Opera, and giving coverage last year to people occupying the empty fourth plinth in Trafalgar Square.
(10) Rodney Mace Hay-on-Wye, Brecknockshire • With the new wall being built in Hungary ( Work begins on border fence to block migrants , 14 July), I am reminded of a conversation I had with a woman whose family has owned, since the 1920s, the apartment where I spent the night a few years ago.
(11) Phenylalanine transfer RNA (tRNAPhe) of mammalian tissues contains the hypermodified guanine derivative Y (Wye) adjacent to the 3'-end of the anticodon and two O-methylated bases in the 5' portion of the anticodon loop.
(12) Under pressure from the Clinton administration, he signed the Wye River Memorandum in October 1998, which included a pledge for an Israeli withdrawal from 13% of the West Bank as a step towards a final status agreement with the Palestinians.
(13) When [methyl-3H5methionine is added in the presence of actinomycin D, which blocks new RNA synthesis, label appears in the wye base of tRNA2Phe.
(14) Measurements of the Wye base fluorescence demonstrate that the isolated anticodon loop of tRNA(Phe) (15 nucleotide residues) has a binding constant K = 75 M-1 for Phe-amide, which is only slightly lower than that observed previously for the complete tRNA(Phe) (K = 100 M-1).
(15) Although the reactions of lithiated 7 with electrophiles gave the 2-substituted derivatives 14, 15, 17, 20, 21, and 22, lithiation of 1-benzyl-7-bromo-2-chlorowye (23) followed by treatment with Me2CHCH2CHO (13) successfully introduced a side chain at the 7-position to afford 1-benzyl-2-chloro-7-(1-hydroxy-3-methylbutyl)wye (24).
(16) It could generate up to 8GW - 5% of the UK's energy needs • Shoots barrage - a scheme further upstream which would generate around 1GW, equivalent to a large fossil fuel plant • Beachley barrage - an even smaller scheme, just above the Wye River, which would generate around 625MW • Bridgwater Bay lagoon - a proposal which would impound a section of the estuary on the coast between east of Hinkley Point and Weston-super-Mare, which could generate 1.36GW • Fleming lagoon - a similar scheme which would generate the same amount of power from a section of the Welsh shore between Newport and the Severn road crossings.
(17) A fluorescent base wye (Yt base) was isolated from Torulopsis utilis tRNAPhe.
(18) Marks & Spencer, however, which claims to be the only UK supermarket selling only British asparagus, says it has 100% availability in all its stores, grown in Herefordshire in the Wye Valley under polytunnels .
(19) The NHS's Health and Social Care Information Centre (HSCIC) on Wednesday said that those three, plus Mid Cheshire Hospitals NHS foundation trust, Aintree University Hospital NHS foundation trust in Liverpool and Wye Valley NHS trust in Herefordshire, all had unusually high death rates in 2012-13, as judged by the summary hospital-level mortality indicator ( SHMI ).
(20) A relaxation effect with a time constant of approximately 100 microseconds, indicated by the Wye base fluorescence, is attributed to a conformational change of the anticodon loop and is very similar to a corresponding transition observed previously for the whole tRNAPhe molecule.