What's the difference between quench and quenchable?
Quench
Definition:
(v. t.) To extinguish; to overwhelm; to make an end of; -- said of flame and fire, of things burning, and figuratively of sensations and emotions; as, to quench flame; to quench a candle; to quench thirst, love, hate, etc.
(v. t.) To cool suddenly, as heated steel, in tempering.
(v. i.) To become extinguished; to go out; to become calm or cool.
Example Sentences:
(1) The extreme quenching of the dioxetane chemiluminescence by both microsomes and phosphatidylcholine, as a model phospholipid, implies that despite the low quantum yield (approx.
(2) The drug is extracted from serum or urine with ethyl acetate, separated by TLC, and determined by fluorescence quenching densitometry.
(3) Formation of a complex between alpha-tocopherol or its analogues in the excited state and fatty acids or their hydroperoxides has been suggested basing on the fluorescence quenching experimental data.
(4) Quenching of intrinsic fluorescence of (Ca2+-Mg2+)-ATPase by acrylamide, performed in the presence of Ca2+, gave evidence for a single class of tryptophan residues with Stern-Volmer constant (KSV) of 10 M-1.
(5) The 23Na double-quantum signal was quenched in both the extracellular and the intracellular compartments with increasing concentration of Li in each compartment, along with an increase in the 23Na T1 both intra- and extracellularly.
(6) Binding increases the fluorescence intensity of Tyr-49 by 130% while the fluorescence of the hormone tyrosine is almost completely quenched.
(7) Addition of 2,6-dimethylbenzoquinone caused quenching of these absorbance changes.
(8) These observations lead to the hypothesis that acidosis quenches fluorescence in distal skin flaps.
(9) The degree of quenching was accurately predicted by a simple relation derived in this paper, as well as a more complex equation previously developed by Tweet, et al.
(10) Subtilin may slightly enter the hydrophobic core as suggested by tryptophan fluorescence quenching and liposome fusion experiments.
(11) Greater than 99% of the polymerization reaction products were quenched by the addition of 2.0 mM ascorbate.
(12) Acoustic probe-based assays can enhance assay and laboratory efficiency through testing for multiple analytes in a single sample or increasing available binding surface area (by using probe and well surfaces simultaneously), and by eliminating quenching.
(13) The second-order rate constants appear to be at least 3 orders of magnitude lower than the second-order constants for quenching of the fluorescent probes; this is taken as a clear indication that ubiquinone diffusion is not the rate-determining step in the quinone-enzyme interaction.
(14) Accessibility to iodide was much lower, as was the rate of quenching by iodide, adding support to the conclusions from acrylamide quenching.
(15) An ATP-dependent, N-ethylmaleimide-inhibitable, 3,3',4',5-tetrachlorosalicylanilide-reversible, and chloride-attenuated quench of bis(1,3-dibutylbarbituric acid-(5] pentamethinoxonol fluorescence was seen, consistent with net transfer of positive charge into the vesicles.
(16) Quenching data indicated that five out of 22 tryptophans in CBH are surface-localized and are available for quenching with both KI and acrylamide, and three other tryptophans are buried and are available only to acrylamide.
(17) Acid quenching of a stiochiometric reaction between Ac(= S)CoA and citrate synthase following the transient quantitatively regenerates Ac(= S)CoA, indicating carbon-carbon bond formation had not yet occurred.
(18) The pulsed laser photolysis products of the charge-transfer quenching reaction were examined.
(19) The highest yield of amino acids with the quench reaction was 9 x 10-7 molecules per erg of input energy.
(20) Tris-washed chloroplast enriched in the photosystem II reaction center species Z+Q- and ZQ- are nearly four times more sensitive to nitrobenzene quenching than those enriched in Z+Q.
Quenchable
Definition:
(a.) Capable of being quenched.
Example Sentences:
(1) The most important effect is produced by beta subunit-mutation which decreases the quenchable fraction, one-third to one-half tryptophans being no longer accessible to iodide.
(2) Denaturation with urea or guanidine HCl plus dithiothreitol increases the fraction of quenchable fluorescence even more, but still a small fraction, about 7-13%, is buried.
(3) To more precisely define the position of the quenchable tryptophan, the experiment was repeated with lipids with the bromine atoms at the 4,5-, 6,7- or 9,10-positions.
(4) Stern-Volmer plots of the quenching data are nonlinear and indicate that there is more than one quenchable conformation of the calcium-free protein.
(5) In contrast, upon complete removal of calcium, the fluorescence is completely quenchable as shown by extrapolation of the data to infinite iodide concentration.
(6) Although the enzyme contains three tryptophan residues, Stern-Volmer plots are linear with all the fluorescence quenchable at high acrylamide concentrations.
(7) Phosphorescent probes are described that are quenchable by dioxygen and that partition into membranes.
(8) A model is presented in which the quenchability of a buried tryptophan is inversely related to the distance from the surface when diffusion through the protein is the rate-limiting step.
(9) A small decrease was observed in the quenchability of a fluorescent probe attached to the sulphydryl group and a small increase in the uptake of an hydrophobic probe.
(10) However, as opposed to native S-1, in heat-treated S-1 the addition of ATP does not induce an increase in tryptophan fluorescence, and, in the case of the treated species, the fluorescence of 1,N6-ethenoadenosine 5'-diphosphate added to the mixture is quenchable by acrylamide.
(11) Apparent quenching constants are wavelength-dependent; global analysis reveals a quenchable component corresponding to the 4.5 ns component and an "unquenchable" component superposing the 0.6-ns spectrum.
(12) At pH values close to the isoelectric points of the enzymes the electrostatic parameter suggests that the environment of the quenchable tyrosines in L-asparaginase is somewhat more positive than in ribonuclease.
(13) A really remarkable finding of studies of protein phosphorescence is that there is such variability both in phosphorescence lifetime and quenchability.
(14) The differential effectiveness of external quenchers on unliganded and liganded CPK allows the determination of the ligand binding equilibria by fluorescence-quenchability titration.
(15) Plaques are counted under a fluorescence microscope and the preparations can be made permanent and virtually non-quenchable by drying.